Methoxy-X04 |
| カタログ番号GC10894 |
蛍光性アミロイドβ（Aβ）プローブ
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 863918-78-9
Sample solution is provided at 25 µL, 10mM.
Methoxy-X04 is a fluorescent derivative of Congo Red and Thioflavin T, lacking acidic groups, with a smaller molecular weight and increased lipophilicity. Methoxy-X04 can cross the blood-brain barrier and selectively binds to the beta-sheet structures in dense core amyloid-beta (Aβ) plaques. Methoxy-X04 retains its in vitro binding affinity for amyloid-beta (Aβ) protofibrils, Ki=26.8nM. In vitro, Methoxy-X04 can stain plaques, tangles, and cerebral amyloid angiopathy in post-mortem Alzheimer's disease (AD) brain sections, demonstrating good specificity. In vivo, Methoxy-X04 can be used for imaging Aβ amyloid plaques in the brains of living APP/PS1 mice [1,2].
References:
[1] Bisht K, et al. Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques. J Vis Exp. 2016 Jun 1;(112):54060.
[2] Klunk WE, et al. Imaging Abeta plaques in living transgenic mice with multiphoton microscopy and methoxy-X04, a systemically administered Congo red derivative. J Neuropathol Exp Neurol. 2002 Sep;61(9):797-805.
This plan for Methoxy X04 serves as a guide， please modify it to suit your specific needs.
1. Prepare dyeing solution
Solvent: 10% DMSO, 45% propylene glycol and 45% phosphate buffered saline (pH7.5, 0.01M, 5mg/ml)
(1) Use a microbalance to weigh 5 mg of Methoxy-X04, dissolve Methoxy-X04 in DMSO under a fume hood and stir until a clear green solution is obtained;
(2) Add propylene glycol and phosphate buffered saline in sequence, stirring each time;
(3) Place the solution on a rotator and stir at 4°C until it becomes a yellow-green emulsion;
2. Animal injection: The experimental animals are mice as an example. Methoxy-X04 is administered via intraperitoneal injection at a dose of 10 mg/kg.
3. For tissue frozen sections, the recommended sample thickness for Methoxy-X04 is 40-50 µm.
4. Examine the sections under a fluorescence microscope to identify areas containing methoxy-X04-labeled Aβ plaques. NOTE: Methoxy-X04 can be easily observed using a fluorescent ultraviolet (UV) filter (excitation wavelength 340 - 380 nm).
Precautions:
1) Fluorescent dyes all have quenching problems. Please avoid light as much as possible to slow down fluorescence quenching.
2) For your safety and health, please wear a lab coat and disposable gloves.
References:
[1]. Kanchan Bisht,et,al. Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques. 2016 Jun 1:(112):54060. doi: 10.3791/54060.
[2]. Marcin Sadowski,et,al. Targeting prion amyloid deposits in vivo. 2004 Jul;63(7):775-84. doi: 10.1093/jnen/63.7.775.
| Cas No. | 863918-78-9 | SDF | |
| Chemical Name | 4,4'-((1E,1'E)-(2-methoxy-1,4-phenylene)bis(ethene-2,1-diyl))diphenol | ||
| Canonical SMILES | COC1=C(C=CC(/C([H])=C([H])/C2=CC=C(O)C=C2)=C1)/C([H])=C([H])/C3=CC=C(O)C=C3 | ||
| Formula | C23H20O3 | M.Wt | 344.4 |
| 溶解度 | 5 mg/ml in ethanol; 30 mg/ml in DMSO and DMF | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.9036 mL | 14.518 mL | 29.036 mL |
| 5 mM | 0.5807 mL | 2.9036 mL | 5.8072 mL |
| 10 mM | 0.2904 mL | 1.4518 mL | 2.9036 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
-
Related Biological Data
HD alleviates Aβ pathology in transgenic C. elegans. (C) Methoxy-X04 was used for Aβ staining.
Methoxy-X04 was used to stain Aβ plaques (Meilandt et al., 2019) in GMC101.The synchronized L4 stage nematodes were administered drugs at 16 °C for 36 h, then transferred to 25 °C for 24 h to induce Aβ formation, and the surviving nematodes were collected and placed in Methoxy-X04(GLPBIO)(1 mM in 10 mM Tris, pH=8.0) for staining 4 h.
Phytomedicine (2023): 154711. PMID: 36809694 IF: 6.6563 -
Related Biological Data
The effect of MG against Aβ-induced pathological and behavioral injury was blocked by GW9662 (20 μM). (A) Aβ deposits were stained by methoxy-X04 in GMC101.
Live worms were collected and incubated in methoxy-X04 solution (GLPBIO)(1 mM in 10 mM Tris, pH8.0) staining for 4 h at 25 °C, and then transferred to seeded NGM plates for 12 h to allow destaining via normal metabolism.
Biomed Pharmacother 124 (2020): 109886. PMID: 32000045 IF: 6.524
Average Rating: 5 (Based on Reviews and 26 reference(s) in Google Scholar.)
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