Oxytocin |
| カタログ番号GC16222 |
哺乳動物の神経下垂体ホルモン
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 50-56-6
Sample solution is provided at 25 µL, 10mM.
;)
_1-7.$$$37316672@70.jpg');)
;)
;)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
;)
;)
;)
_1124-1138.$$$35908550@30.jpg');)
_766-780.$$$37100057@30.jpg');)
_418-432.$$$36893736@30.jpg');)
_240-255.$$$35108512@29.jpg');)
_533-545.$$$36543525@28.jpg');)
_264-276.$$$36600053@22.jpg');)
_2214998.$$$@0.jpg');)
Oxytocin is a pleiotropic hypothalamic peptide that helps in childbirth, lactation and prosocial behavior[1]. Oxytocin can act as a stress response molecule with anti-inflammatory, antioxidant and neuroprotective effects[2]. Oxytocin can regulate the growth of cancer cells by activating oxytocin receptors[3].
In vitro, Oxytocin (1μM) treatment of SH-SY5Y cells for 12h significantly increased the amount of total protein in cell lysates and significantly reduced the concentration of neurotrophic growth factor (NGF)[4]. Treatment of human umbilical vein endothelial cells (HUVEC) with Oxytocin (1nM) stimulated cell migration and invasion[5]. Treatment of hTERTmyo and SK-UT-1 cells with Oxytocin (1μM) for 24-96h inhibited the proliferation of both cells in a time-dependent manner[6].
In vivo, Oxytocin (0.2mg/kg) was administered intranasally to treat valproic acid (VPA)-induced autistic mice, which improved the behavior of autistic mice, reduced anxiety, depression, and repetitive behaviors, and improved social interactions[7]. Oxytocin (0.1-0.3mg/kg) was administered subcutaneously to treat phencyclidine-induced hyperactive rats, which significantly reduced the hyperactive behavior of the rats and increased prosocial behavior[8].
References:
[1] Tarsha M S, Narvaez D. The evolved nest, oxytocin functioning, and prosocial development[J]. Frontiers in Psychology, 2023, 14: 1113944.
[2] Kamrani-Sharif R, Hayes A W, Gholami M, et al. Oxytocin as neuro-hormone and neuro-regulator exert neuroprotective properties: a mechanistic graphical review[J]. Neuropeptides, 2023, 101: 102352.
[3] Cassoni P, Sapino A, Marrocco T, et al. Oxytocin and oxytocin receptors in cancer cells and proliferation[J]. Journal of neuroendocrinology, 2004, 16(4): 362-364.
[4] Bakos J, Strbak V, Ratulovska N, et al. Effect of oxytocin on neuroblastoma cell viability and growth[J]. Cellular and molecular neurobiology, 2012, 32: 891-896.
[5] Cattaneo M G, Chini B, Vicentini L M. Oxytocin stimulates migration and invasion in human endothelial cells[J]. British journal of pharmacology, 2008, 153(4): 728-736.
[6] Busnelli M, Rimoldi V, Viganò P, et al. Oxytocin-induced cell growth proliferation in human myometrial cells and leiomyomas[J]. Fertility and sterility, 2010, 94(5): 1869-1874.
[7] Wang Y, Zhao S, Liu X, et al. Oxytocin improves animal behaviors and ameliorates oxidative stress and inflammation in autistic mice[J]. Biomedicine & Pharmacotherapy, 2018, 107: 262-269.
[8] Kohli S, King M V, Williams S, et al. Oxytocin attenuates phencyclidine hyperactivity and increases social interaction and nucleus accumben dopamine release in rats[J]. Neuropsychopharmacology, 2019, 44(2): 295-305.
| Cell experiment [1]: | |
Cell lines | SH-SY5Y cells |
Preparation Method | Cells were incubated in presence of Oxytocin (1μM) for 12 or 96h. Brain-derived neurotrophic factor (BDNF) or Neurotrophic growth factor (NGF) was measured by ELISA. |
Reaction Conditions | 1μM; 12, 96h |
Applications | Neurotrophic Factors Oxytocin (1μM, 12h) significantly increased amount of total proteins measured in SH-SY5Y cell lysates. Concentration of BDNF related to proteins did not change significantly, while that of NGF significantly decreased in response to Oxytocin. No effect of Oxytocin on neurotrophic factors was observed after 24 or 48h. |
| Animal experiment [2]: | |
Animal models | C57BL/6J mice |
Preparation Method | The pregnant mice were randomly divided into 2 groups: the control group and valproate (VPA) group. At E12.5, pregnant mice in the VPA group received VPA (600mg/kg; intraperitoneal injection) to induce autism of offsprings. Pregnant mice in the control group received equal amounts of normal saline. Male offspring were used for subsequent experiments. After identification of autism, the offspring were divided into 3 groups: the control group, autism group and autism+Oxytocin group. At postnatal day 30 (P30), offspring in autism+Oxytocin group received Oxytocin (0.2mg/kg; intranasal administration). 30min later, animal behavior tests were performed. |
Dosage form | 0.2mg/kg; intranasal administration |
Applications | Oxytocin improved the behaviors of autistic mice, with less anxiety, depression and repetitive behavior, and ameliorated social interaction. |
References: | |
| Cas No. | 50-56-6 | SDF | |
| Formula | C43H66N12O12S2 | M.Wt | 1007.19 |
| 溶解度 | ≥ 182.8mg/mL in DMSO | Storage | Store at 4°C, stored under nitrogen |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 0.9929 mL | 4.9643 mL | 9.9286 mL |
| 5 mM | 0.1986 mL | 0.9929 mL | 1.9857 mL |
| 10 mM | 0.0993 mL | 0.4964 mL | 0.9929 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 20 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *