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Pyrene phosphoramidite dU

カタログ番号GC14718

蛍光オリゴDNAプローブ

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Pyrene phosphoramidite dU 化学構造

Cas No.: 199920-17-7

サイズ 価格 在庫数 個数
100mg
$107.00
在庫あり

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Sample solution is provided at 25 µL, 10mM.

Description of Pyrene phosphoramidite dU

Pyrene is polycyclic aromatic hydrocarbon which is well-known for its ability to intercalate into DNA. Pyrene features intense blue fluorescence. Its parameters depend strongly on fluorophore microenvironment. Therefore, fluorescence spectra of pyrene are used for the extraction of structural information about site surrounding pyrene. Two pyrenes in close proximity usually form excimers easily detectable by excimer fluorescence. Pyrene can also be a FRET donor to other fluorophores such as perylene. With this phosphoramidite, pyrene can be introduced into DNA or RNA by means of automated synthesis. This pyrene phosphoramidite contains hydrocarbon moiety rigidly attached to deoxyuridine. This reagent allows to attach pyrene fragment to 5', internal, or 3' position (using universal support). This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent for it is acetonitrile. Pyrene dU amidite

Chemical Properties of Pyrene phosphoramidite dU

Cas No. 199920-17-7 SDF
Chemical Name 3-[[(2R,3R,5R)-5-[5-[2-(8,10b-dihydropyren-1-yl)ethynyl]-2,4-dioxopyrimidin-1-yl]-2-[bis(4-methoxyphenyl)methoxymethyl]oxolan-3-yl]oxy-[di(propan-2-yl)amino]phosphanyl]oxypropanenitrile
Canonical SMILES CC(C)N(C(C)C)P(OCCC#N)OC1CC(OC1COC(C2=CC=C(C=C2)OC)C3=CC=C(C=C3)OC)N4C=C(C(=O)NC4=O)C#CC5=CC=C6C=CC7=C8C6C5=CC=C8CC=C7
Formula C57H55N4O8P M.Wt 955.04
溶解度 good in dichloromethane and acetonitrile Storage 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of Pyrene phosphoramidite dU

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1 mg 5 mg 10 mg
1 mM 1.0471 mL 5.2354 mL 10.4708 mL
5 mM 0.2094 mL 1.0471 mL 2.0942 mL
10 mM 0.1047 mL 0.5235 mL 1.0471 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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