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(S)-Bromoenol lactone-d7 (Synonyms: (S)BELd7)

カタログ番号GC46350

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(S)-Bromoenol lactone-d7 化学構造

Cas No.: 478288-94-7

サイズ 価格 在庫数 個数
100 μg
$49.00
在庫あり
500 μg
$225.00
在庫あり
1 mg
$398.00
在庫あり

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カスタマーレビューに基づきます。

Sample solution is provided at 25 µL, 10mM.

Description of (S)-Bromoenol lactone-d7

(S)-Bromoenol lactone-d7 ((S)-BEL-d7) contains seven deuterium atoms at the 2, 3, 4, 5, 6, 7, and 8 positions. It is intended for use as an internal standard for the quantification of (S)-BEL by GC- or LC-mass spectrometry (MS). The phospholipases are an extensive family of lipid hydrolases that function in cell signaling, digestion, membrane remodeling, and as venom components.1 The calcium-independent phospholipase A2 (iPLA2) are a PLA2 subfamily closely associated with the release of arachidonic acid in response to physiologic stimuli. (S)-Bromoenol lactone ((S)-BEL) is an irreversible, chiral, mechanism-based inhibitor of iPLA2β that inhibits the vasopressin-induced release of arachidonate from cultured rat aortic smooth muscle (A10) cells with an IC50 value of 2 µM.2 (S)-BEL is more than 1,000-fold selective for iPLA2 versus cPLA2, and is 10-fold selective for iPLA2β versus iPLA2γ.

1.Balsinde, J., Balboa, M.A., Insel, P.A., et al.Regulation and inhibition of phospholipase A2Annual Reviews of Pharmacology and Toxicology39175-189(1999) 2.Jenkins, C.M., Han, X., Mancuso, D.J., et al.Identification of calcium-independent phospholipase A2 (iPLA2)β, and not iPLA2γ, as the mediator of arginine vasopressin-induced arachidonic acid release in A-10 smooth muscle cellsThe Journal of Biological Chemisty277(36)32807-32814(2002)

Chemical Properties of (S)-Bromoenol lactone-d7

Cas No. 478288-94-7 SDF
同義語 (S)BELd7
Canonical SMILES [2H]C1=C([2H])C([2H])=C2C(C([2H])=C([2H])C([2H])=C2[C@@]3([H])C(O/C(CC3)=C/Br)=O)=C1[2H]
Formula C16H6BrD7O2 M.Wt 324.2
溶解度 DMF: 50 mg/ml,DMSO: 25 mg/ml,Ethanol: 5 mg/ml,PBS (pH 7.2): .05 mg/ml Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of (S)-Bromoenol lactone-d7

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 3.0845 mL 15.4226 mL 30.8452 mL
5 mM 616.9 μL 3.0845 mL 6.169 mL
10 mM 308.5 μL 1.5423 mL 3.0845 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.

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