Saccharin 1-methylimidazole (SMI) |
| カタログ番号GC14808 |
サッカリン 1-メチルイミダゾール (SMI) は、DNA/RNA 合成の活性化剤です。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 482333-74-4
Sample solution is provided at 25 µL, 10mM.
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Saccharin 1-methylimidazole (SMI) is a DNA and RNA synthesis activator.
Saccharin 1-methylimidazole (SMI) is the saccharin salt of 1-methylimidazole. 1-Methylimidazole or N-methylimidazole is identified as an aromatic heterocyclic organic agent with the formula CH3C3H3N2. 1-Methylimidazole is a colourless liquid that is used as a base, a specialty solvent, and as a precursor to some ionic liquids. 1-Methylimidazole is a fundamental nitrogen heterocycle and 1-methylimidazole mimics for a variety of nucleoside bases as well as histidine and histamine.
In vitro: Saccharin 1-methylimidazole was found to be able to perform very well for DNA and RNA synthesis. In addition, by using 3 minute, 6 minute, and 12 minute coupling times, saccharin 1-methylimidazole was evaluated in RNA synthesis using TBDMS phosphoramidites, and the results found that a 6 minute coupling time for saccharin 1-methylimidazole showed ETT at a 6 minute coupling time. In additio, it was found that longer coupling times for Saccharin 1-methylimidazole could result in even further improvements in RNA coupling efficiencies [1].
In vivo: So far, there is no animal in vivo data reported for saccharin 1-methylimidazole.
Clinical trial: Up to now, saccharin 1-methylimidazole is not used clinically.
Reference:
[1] Russell MA, Laws AP, Atherton JH, Page MI. The mechanism of the phosphoramidite synthesis of polynucleotides. Org Biomol Chem. 2008 Sep 21;6(18):3270-5.
| Cas No. | 482333-74-4 | SDF | |
| Chemical Name | 1-methyl-1H-imidazol-1-ium benzo[d]isothiazol-3-olate 1,1-dioxide | ||
| Canonical SMILES | [O-]C1=NS(C2=CC=CC=C21)(=O)=O.C[NH+]3C=CN=C3 | ||
| Formula | C11H11N3O3S | M.Wt | 265.29 |
| 溶解度 | DMF: 5mg/mL,DMSO: 5mg/mL,Ethanol: 10mg/mL,PBS (pH 7.2): 10mg/mL | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.7695 mL | 18.8473 mL | 37.6946 mL |
| 5 mM | 0.7539 mL | 3.7695 mL | 7.5389 mL |
| 10 mM | 0.3769 mL | 1.8847 mL | 3.7695 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 13 reference(s) in Google Scholar.)
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