Scrambled 10Panx (Synonyms: Scrambled 10Panx Phe-Ser-Val-Tyr-Trp-Ala-Gln-Ala-Asp-Arg) |
カタログ番号GC11058 |
Panx-1ミメティック阻害ペプチドは、パンネキシン-1ギャップジャンクションをブロックします。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1315378-72-3
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment [1]: | |
Cell lines |
Cultured human subcutaneous fibroblasts. |
Preparation method |
Soluble to 0.50 mg/ml in sterile water. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
Reaction Conditions |
100 μM. |
Applications |
In cultured human subcutaneous fibroblasts, 10panx decreases the bradykinin-induced [Ca2+]i response and attenuates bradykinin (30 μM)-induced ATP release [1]. In oocytes, 10panx inhibits pannexin1 currents. In oocytes expressing pannexin1, 10panx attenuates dye uptake by pannexin1 channels [2]. |
References: [1]. Pinheiro AR, Paramos-de-Carvalho D, Certal M, et al. Bradykinin-induced Ca2+ signaling in human subcutaneous fibroblasts involves ATP release via hemichannels leading to P2Y12 receptors activation. Cell Commun Signal, 2013, 11: 70. [2]. Wang J, Ma M, Locovei S, et al. Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters. Am J Physiol Cell Physiol. 2007, 293(3): C1112-1119. |
スクランブルされた10Panxは、パネキシン1の模倣ペプチドである10Panx(WRQAAFVDSY)のスクランブル形です。このペプチドは、細胞膜電流に影響を与えずにマクロファージの染料取り込みを抑制します。アンテルペプチドPanxE1Bと構造的に類似しているが、3つのアミノ酸が短くなっていますが、卵母細胞中でパネキシン1電流を同等程度に抑制する効果を発揮します。 10Panxはまた、I2ndの活性化を阻止し、ブラジキニン誘導性[Ca2+] i sub>応答を減少させ、キナクリンでロードされた人間線維芽細胞からのブラジキニン誘発性ATP放出も低下させます。ただし、いくつかの研究では、スクランブルした10Panxは本来の形式よりも効果が低いことが証明されています。
参考文献
[1]. Roger J. Thompson、Michael F. Jackson、Michelle E. Olah、Ravi L. Rungta、Dustin J. Hines、Michael A. Beazely、John F. MacDonald、Brian A.MacVicar。パネキシン-1ヘミチャネルの活性化による海馬の異常なバースト増強。Science 2008;322:1555-1559。
[2]. Ana Rita Pinheiro, Diogo Paramos-de-Carvalho, Marianna Certal, Cristina Costa, Maria Teresa Magalhaes-Cardoso, Fatima Ferreirinha, Maria Adelina Costa and Paulo Correia-de-Sa。ブラジキニンによる人間皮下線維芽細胞のCa2+シグナリングは、P2Y12受容体の活性化を引き起こすためにヘミチャンネルを介したATP放出を含む。Communication and Signaling 2013年11月70号
[3]. Junjie Wang、Meiyun Ma、Silviu Locovei、Robert W.KeanおよびGerhard Dahl。ギャップジャンクションタンパク質模倣ペプチドによる膜チャネル電流の調節:サイズが重要です。Am J Physiol Cell Physiol 293:C1112-C1119,2007
Cas No. | 1315378-72-3 | SDF | |
同義語 | Scrambled 10Panx Phe-Ser-Val-Tyr-Trp-Ala-Gln-Ala-Asp-Arg | ||
Canonical SMILES | CC(C)C(C(=O)NC(CC1=CC=C(C=C1)O)C(=O)NC(CC2=CNC3=CC=CC=C32)C(=O)NC(C)C(=O)NC(CCC(=O)N)C(=O)NC(C)C(=O)NC(CC(=O)O)C(=O)NC(CCCN=C(N)N)C(=O)O)NC(=O)C(CO)NC(=O)C(CC4=CC=CC=C4)N | ||
Formula | C58H79N15O16 | M.Wt | 1242.37 |
溶解度 | ≥ 31.05mg/mL in DMSO | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 0.8049 mL | 4.0246 mL | 8.0491 mL |
5 mM | 0.161 mL | 0.8049 mL | 1.6098 mL |
10 mM | 0.0805 mL | 0.4025 mL | 0.8049 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
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