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Tetramethylrhodamine methyl ester (perchlorate) (Synonyms: TMRM, TMR methyl ester)

カタログ番号GC19476

テトラメチルローダミン メチル エステル (過塩素酸塩) は、細胞透過性のカチオン性親油性赤色蛍光色素です (Λex=530 nm、Λem=592 nm)。

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Tetramethylrhodamine methyl ester (perchlorate) 化学構造

Cas No.: 115532-50-8

サイズ 価格 在庫数 個数
5mg
$48.00
在庫あり
10mg
$72.00
在庫あり
25mg
$133.00
在庫あり
50mg
$266.00
在庫あり

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Cell experiment:

Cultures are exposed to Millipore-filtered solutions (0.22 µm) containing TMRM Perchlorate for 1 hr at 37°C (except the experiment involving different durations of exposure to TMRM Perchlorate). After treatment, solutions are removed and growth media reapplied under sterile conditions, and cultures are post-incubated for 18 hours at 37°C (except for the experiment involving analysis at different time points after exposure). Cells are then stained with 2 mg/mL bisbenzimide for 20 min at room temperature. Coverslips are subsequently washed in saline and imaged using 2P microscopy. Apoptotic cells are identified as brightly fluorescent nuclei under UV excitation indicating DNA fragmentation. Cell survivability is calculated as the percentage of live, unstained cells (±SD) in five microscopic fields per treatment[1].

References:

[1]. Chowdhury SR, et al. Simultaneous evaluation of substrate-dependent oxygen consumption rates and mitochondrial membrane potential by TMRM and safranin in cortical mitochondria. Biosci Rep. 2015 Dec 8;36(1):e00286.
[2]. Monteith A, et al. Imaging of mitochondrial and non-mitochondrial responses in cultured rat hippocampal neurons exposed to micromolar concentrations of TMRM. PLoS One. 2013;8(3):e58059.

Background

Tetramethylrhodamine methyl ester (TMRM) (perchlorate) is a non-cytotoxic cell-permeant fluorogenic dye most commonly used to assess mitochondrial function using live cell fluorescence microscopy and flow cytometry.1,2,3 It has two excitation peaks at 515 and 555 nm and an emission peak in the red-orange range (575 nm). Due to the polarization of the mitochondrial membrane, TMRM is taken up into healthy mitochondria. However, when the membrane is depolarized, as in apoptosis, it is not taken up or is released from the mitochondria. Thus, the strength of the fluorescence signal in mitochondria is used to assess cell viability.

Reference: 

1. Farkas, D.L., Wei, M.-d., Febbroriello, P., et al. Simultaneous imaging of cell and mitochondrial membrane potentials Biophys J. 56(6), 1053-1069 (1989).
2. Gandra, P.G., Nogueira, L., and Hogan, M.C. Mitochondrial activation at the onset of contractions in isolated myofibres during successive contractile periods J. Physiol. 590(15), 3597-3609 (2012).
3. Michea, L., Combs, C., Andrews, P., et al. Mitochondrial dysfunction is an early event in high-NaCl-induced apoptosis of mIMCD3 cells Am. J. Physiol. Renal Physiol. 282(6), F981-F990 (2002).

Chemical Properties

Cas No. 115532-50-8 SDF
同義語 TMRM, TMR methyl ester
Chemical Name 3,6-bis(dimethylamino)-9-[2-(methoxycarbonyl)phenyl]-xanthylium, perchlorate
Canonical SMILES O=C(OC)C(C=CC=C1)=C1C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4.O=Cl(=O)([O-])=O
Formula C25H25N2O3 • ClO4 M.Wt 500.9
溶解度 DMF: 30 mg/ml,DMSO: 30 mg/ml,Ethanol: slightly soluble,PBS (pH 7.2): slightly soluble Storage Store at -20°C, protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 1.9964 mL 9.982 mL 19.9641 mL
5 mM 0.3993 mL 1.9964 mL 3.9928 mL
10 mM 0.1996 mL 0.9982 mL 1.9964 mL
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計算

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 14 reference(s) in Google Scholar.)

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