>>2',3'-cGAMP

2',3'-cGAMP (Synonyms: 2'-3'-cyclic GMP-AMP)

Catalog No.GC19989

2',3'-cGAMP(2'-3'-사이클릭 GMP-AMP)는 포유동물 세포의 내인성 cGAMP입니다.

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2',3'-cGAMP Chemical Structure

Cas No.: 1441190-66-4

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1mg
US$270.00
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5mg
US$1,035.00
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10mg
US$1,800.00
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Cell experiment [1]:

Cell lines

PC3 cell

Preparation method

Cell toxicity was measured using the method of MTT. After 24 hours of incubation, cells were exposed to docetaxel for 48 hours at concentrations of 2.2, 4.4, 8.75, 17.5 and 35 μM 2',3'-cGAMP. After 48h incubation, the cell suspension was washed with PBS.

Reaction Conditions

2.2, 4.4, 8.75, 17.5 and 35 μM; 48h

Applications

The 2',3'-cGAMP has cytotoxic effects, IC50 was 11.46 μM.

Animal experiment [2]:

Animal models

C57 BL/6 mice

Preparation method

After melanoma cell inoculation, C57 BL/6 mice were treated with 3 μg, 10 μg of 2′3′-cGAMP or 200 μg of PD-L1 antibody on days 4, 8 and 12 respectively (n = 4-5 in each group). A total of 50 μL of 2′3′-cGAMP in PBS at the indicated concentrations was injected into the muscles of the hind legs, and the tumor volume was measured.

Dosage form

3 μg/10 μg;4, 8 and 12days;i.m.

Applications

10 μg of 2',3'-cGAMP was as effective as 200 μg of the PD-L1 antibody, and the combination treatment was even more effective. Titration experiment showed that 2',3'-cGAMP  enhanced the antitumor effects of PD-L1 antibody in a dose-dependent manner.

References:

[1] Rezaei M .09-P53. Docetaxel Enhances the Expression of STING Protein in PC3 Prostate Cancer Cells, and cGAMP Attenuates This Effect[C]. Benchmarking an Artificial Intelligence Method for Fast Diagnosis of Rare Genetic Disease.2020.

[2] Wang, H. et al. cGAS is essential for the antitumor effect of immune checkpoint blockade. Proc. Natl Acad. Sci. USA 114, 1637–1642 (2017).

Background

2',3'-cGAMP is a specific agonist of the STING receptor, and its mechanism of action primarily involves activating the STING signaling pathway, promoting the production of interferons and inflammatory cytokines. 2',3'-cGAMP plays an important role in the innate immune pathway of mammals, responsible for the surveillance of cytoplasmic DNA. The Kd value of 2',3'-cGAMP binding to STING is 3.79 nM[1]. 2',3'-cGAMP is commonly used in research on immune regulation, antiviral immunity, and cancer treatment.

In vitro, 2',3'-cGAMP (2.2, 4.4, 8.75, 17, 35 μM) treatment of PC 3 cells for 48 hours has an IC50 value of 11.46 μM[2]. Treating the PC 3 cell line with 2',3'-cGAMP (4 µg/ml) for 10 minutes activates STING[3].

In vivo, 10 μg of 2',3'-cGAMP is as effective as 200 μg of PD-L1 antibody in combating melanoma in C57 BL/6 mice. Titration experiments show that 2',3'-cGAMP enhances the antitumor effect of the PD-L1 antibody in a dose-dependent manner[4].

References:

[1] Zhang X, et al. Cyclic GMP-AMP containing mixed phosphodiester linkages is an endogenous high-affinity ligand for STING. Mol Cell. 2013 Jul 25;51(2):226-35. 

[2] Rezaei M .09-P53. Docetaxel Enhances the Expression of STING Protein in PC3 Prostate Cancer Cells, and cGAMP Attenuates This Effect[C]. Benchmarking an Artificial Intelligence Method for Fast Diagnosis of Rare Genetic Disease.2020.

[3] Hannes S , Karlowitz R , Wijk S J L V .The Smac mimetic BV6 cooperates with STING to induce necroptosis in apoptosis-resistant pancreatic carcinoma cells[J].Cell Death & Disease.2024.

[4] Wang, H. et al. cGAS is essential for the antitumor effect of immune checkpoint blockade. Proc. Natl Acad. Sci. USA 114, 1637–1642 (2017).

Chemical Properties

Cas No. 1441190-66-4 SDF
Synonyms 2'-3'-cyclic GMP-AMP
Formula C20H24N10O13P2 M.Wt 674.41
Solubility Water : 5 mg/mL (7.41 mM; ultrasonic and warming and heat to 60°C) Storage -20°C, sealed storage, away from moisture
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 1.4828 mL 7.4139 mL 14.8278 mL
5 mM 0.2966 mL 1.4828 mL 2.9656 mL
10 mM 0.1483 mL 0.7414 mL 1.4828 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 30 reference(s) in Google Scholar.)

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