2',3'-cGAMP (Synonyms: 2'-3'-cyclic GMP-AMP) |
| Catalog No.GC19989 |
2' ,3' -cGAMP는 STING 수용체의 특정 작용제이고 그 작용 메커니즘은 주로 STING 신호 경로를 활성화하여 인터페론 및 염증 사이토카인의 생성을 촉진하는 것이다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1441190-66-4
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
2' ,3' -cGAMP는 STING 수용체의 특정 작용제이고 그 작용 메커니즘은 주로 STING 신호 경로를 활성화하여 인터페론 및 염증 사이토카인의 생성을 촉진하는 것이다. 2',3'-cGAMP는 포유류의 선천 면역 경로에서 중요한 역할을 하고 세포질 DNA를 감시하는 역할을 한다. 2',3'-cGAMP가 STING에 결합하는Kd 값은 3.79 nM이다[1]. 2',3'-cGAMP는 면역 조절, 항바이러스 면역 및 암 치료 연구에 일반적으로 사용된다.
체외 실험에서 2',3'-cGAMP（2.2, 4.4, 8.75, 17, 35 μM）가 PC-3 세포에 48시간 처리된 후의 IC50 값은 11.46 μM이다[2]. 2',3'-cGAMP（4 µg/ml）로 PC-3 세포 라인을 10분간 처리하면 STING을 활성화한다[3].
체내 실험에서 2',3'-cGAMP의 10 μg가 C57 BL/6 쥐의 멜라놈마를 싸우는데 200 μg의 PD-L1 항체만큼 효과적이다. 적정 실험은 2',3'-cGAMP가 PD-L1 항체의 항종 효과를 용량 의존적인 방식으로 증강시킨다고 보여준다[4].
References:
[1] Zhang X, et al. Cyclic GMP-AMP containing mixed phosphodiester linkages is an endogenous high-affinity ligand for STING. Mol Cell. 2013 Jul 25;51(2):226-35.
[2] Rezaei M .09-P53. Docetaxel Enhances the Expression of STING Protein in PC3 Prostate Cancer Cells, and cGAMP Attenuates This Effect[C]. Benchmarking an Artificial Intelligence Method for Fast Diagnosis of Rare Genetic Disease.2020.
[3] Hannes S , Karlowitz R , Wijk S J L V .The Smac mimetic BV6 cooperates with STING to induce necroptosis in apoptosis-resistant pancreatic carcinoma cells[J].Cell Death & Disease.2024.
[4] Wang, H. et al. cGAS is essential for the antitumor effect of immune checkpoint blockade. Proc. Natl Acad. Sci. USA 114, 1637–1642 (2017).
| 세포 실험 [1]: | |
세포 라인 | PC3세포 |
제조 방법 | 세포 독성을 MTT 방법으로 측정했습니다. 24시간 배양 후 세포는 다양한 농도의 도세타졸(2.2, 4.4, 8.75, 17.5, 35 μM 2',3'-cGAMP)에 48시간 동안 노출되었습니다. 48시간 배양 후 세포 서지를 PBS로 씻겼습니다. |
반응 조건 | 2.2, 4.4, 8.75, 17.5및35 μM; 48시간 동안 |
응용 분야 | 2', 3'-cGAMP은 세포 독성 효과, IC50 은 11.46 μM입니다. |
| 동물 실험 [2]: | |
동물 모형 | C57 BL/6 쥐 |
제조 방법 | 멜라놈세포 접종 후 C57 BL/6 쥐는 각각 4일, 8일, 12일에 3 μg, 10 μg의 2′3′-cGAMP 또는 200 μg의 PD-L1 항체를 치료했습니다 (각 그룹에서 n = 4-5). 지정된 농도의 PBS에 50 μL의 2′3′-cGAMP를 후다리 근육에 주입했으며, 종양 부피를 측정했습니다. |
제형 | 3 μg/10 μg；4, 8 및 12일 동안；i.m. |
응용 분야 | 10 μg의 2',3'-cGAMP은 200 μg의 PD-L1 항체만큼 효과적이었고 복합 치료는 더욱 효과적이었습니다. 적정 실험은 2',3'-cGAMP이 PD-L1 항체의 항종 효과를 용량 의존적인 방식으로 증강시킨다고 보여주었습니다. |
| Cas No. | 1441190-66-4 | SDF | |
| Synonyms | 2'-3'-cyclic GMP-AMP | ||
| Formula | C20H24N10O13P2 | M.Wt | 674.41 |
| Solubility | Water : 5 mg/mL (7.41 mM; ultrasonic and warming and heat to 60°C) | Storage | -20°C, sealed storage, away from moisture |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.4828 mL | 7.4139 mL | 14.8278 mL |
| 5 mM | 0.2966 mL | 1.4828 mL | 2.9656 mL |
| 10 mM | 0.1483 mL | 0.7414 mL | 1.4828 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *