>>Signaling Pathways>> Proteases>> Lipoxygenase>>2,5-Dihydroxycinnamic Acid phenethyl ester

2,5-Dihydroxycinnamic Acid phenethyl ester

Catalog No.GC46057

An inhibitor of 5-LO

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2,5-Dihydroxycinnamic Acid phenethyl ester Chemical Structure

Cas No.: 179691-97-5

Size 가격 재고 수량
5mg
US$153.00
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10mg
US$252.00
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25mg
US$531.00
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Sample solution is provided at 25 µL, 10mM.

Description of 2,5-Dihydroxycinnamic Acid phenethyl ester

2,5-Dihydroxycinnamic acid phenethyl ester is an inhibitor of 5-lipoxygenase (5-LO) with an IC50 value of 0.33 μM for inhibition of 5-LO product synthesis in polymorphonuclear leukocytes (PMNLs) stimulated with the sarcoendoplasmic reticulum calcium ATPase (SERCA) inhibitor thapsigargin .1 It scavenges 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals (IC50 = 16.41 μM) in a cell-free assay. 2,5-Dihydroxycinnamic acid phenethyl ester selectively decreases viability of RCC4 and 786-O cells lacking the Von Hippel-Lindau (VHL) tumor suppressor gene (IC50s = 8 and 34.8 μM, respectively) over cells homozygously expressing VHL (IC50s = 99.6 and >100 μM, respectively). However, it also decreases viability of RCC10 VHL-/- and RCC10 VHL+/+ cells (IC50s = 5.04 and 0.96 μM, respectively). It increases the levels of pro-caspase-3a, LC3B-I, and LC3B-II in RCC4 VHL-/- cells in a concentration-dependent manner.

|1. Selka, A., Doiron, J.A., Lyons, P.A., et al. Discovery of a novel 2,5-dihydroxycinnamic acid-based 5-lipoxygenase inhibitor that induces apoptosis and may impair autophagic flux in RCC4 renal cancer cells. Eur. J. Med. Chem. 179, 347-357 (2019).

Chemical Properties of 2,5-Dihydroxycinnamic Acid phenethyl ester

Cas No. 179691-97-5 SDF
Canonical SMILES O=C(OCCC1=CC=CC=C1)/C=C/C2=CC(O)=CC=C2O
Formula C17H16O4 M.Wt 284.3
Solubility DMF: 30 mg/ml,DMSO: 30 mg/ml,Ethanol: 30 mg/ml Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of 2,5-Dihydroxycinnamic Acid phenethyl ester

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 3.5174 mL 17.5871 mL 35.1741 mL
5 mM 0.7035 mL 3.5174 mL 7.0348 mL
10 mM 0.3517 mL 1.7587 mL 3.5174 mL
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In vivo Formulation Calculator (Clear solution) of 2,5-Dihydroxycinnamic Acid phenethyl ester

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 29 reference(s) in Google Scholar.)

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