4-Methylumbelliferyl-β-D-Glucopyranoside (Synonyms: 4-MU-GLU;4-MU-β-Gluc;4-MUG;MU-GLU) |
| Catalog No.GC11870 |
4-Methylumbelliferyl-β-D-Glucopyranoside, a β-D-glucoside, β-glucosidase에 대한 형광 기질입니다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 18997-57-4
Sample solution is provided at 25 µL, 10mM.
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4-Methylumbelliferyl-β-D-Glucopyranoside is a β-D-glucoside that serves as a fluorescent substrate for β-glucosidase, thereby allowing for the detection and analysis of this enzyme's activity[1]. The enzymatic cleavage of 4-Methylumbelliferyl β-D-glucopyranoside results in the release of the highly fluorescent compound 4-Methylumbelliferone. The maximum emission wavelength of 4-MU ranges from 445 to 454nm, while its excitation wavelength varies with pH: at pH 4.6, 7.4, and 10.4, the corresponding excitation wavelengths are approximately 330nm, 370nm, and 385nm, respectively[2].
References:
[1] Van Roey P, Salerno JM. Structure of 4-methylumbelliferyl-beta-D-glucopyranoside. Acta Crystallogr C. 1988 May 15;44 ( Pt 5):865-7.
[2] Mikhaylova M, Wiederschain G, Mikhaylov V, et al. The enzymatic hydrolysis of 6-acylamino-4-methylumbelliferyl-beta-D-glucosides: identification of a novel human acid beta-glucosidase. Biochim Biophys Acta. 1996 Oct 7;1317(1):71-9.
Assay of Proteasome Activity in Cell
This protocol is adapted from research data and provided for reference only. Adjustments may be necessary based on specific experimental requirements.
Enzyme Sample Preparation: Lyse cells and collect the supernatant. Store the clarified lysate at 4°C for subsequent use.
β-Glucosidase Activity Detection: Prepare a reaction mixture containing 5μM 4-Methylumbelliferyl-β-D-Glucopyranoside, 100mM citrate buffer (pH=5.0), total volume: 200μL; Incubate at 45°C for 1 hour. Terminate the reaction by adding 2.5mL of 0.5M Na₂CO₃ buffer (pH=10.4). Detect the product using a fluorometer with an excitation wavelength of 350nm and an emission wavelength of 440nm.
Precautions:
1) Free fluorescent substrates are subject to quenching. Please try to avoid light exposure to slow down fluorescence quenching.
2) For your safety and health, please wear a lab coat and disposable gloves when operating.
References:
[1] Hwangbo J, Lee MJ, Kim SJ, et al. Comparative analysis of methods for measuring glucocerebrosidase enzyme activity in patients with Parkinson's disease with the GBA1 variant. Front Neurol. 2025 Apr 2;16:1523655.
| Cas No. | 18997-57-4 | SDF | |
| Synonyms | 4-MU-GLU;4-MU-β-Gluc;4-MUG;MU-GLU | ||
| Chemical Name | 4-methyl-7-(((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)-2H-chromen-2-one | ||
| Canonical SMILES | OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O1 | ||
| Formula | C16H18O8 | M.Wt | 338.3 |
| Solubility | ≥ 84.5mg/mL in DMSO with gentle warming | Storage | Store at 2-8°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.956 mL | 14.7798 mL | 29.5596 mL |
| 5 mM | 591.2 μL | 2.956 mL | 5.9119 mL |
| 10 mM | 295.6 μL | 1.478 mL | 2.956 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 15 reference(s) in Google Scholar.)
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