>>Signaling Pathways>> DNA Damage/DNA Repair>> Nucleoside Antimetabolite/Analogue>>5-BrdU

5-BrdU (Synonyms: BrdU, Bromodeoxyuridine, Broxuridine, NSC 38297)

Catalog No.GC11940

합성 티미딘 아날로그

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5-BrdU Chemical Structure

Cas No.: 59-14-3

Size 가격 재고 수량
10mM (in 1mL DMSO)
US$42.00
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500mg
US$35.00
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1g
US$58.00
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

View current batch:

Protocol

Vitro experiment [1]:

Samples

Nicotiana pith tissues

Preparation method

The solubility of this compound in DMSO is >15.4mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

5 μg/ml; 24 h

Applications

In nicotiana pith tissues, BrdU inhibited growth of pith explants, while thymidine completely reversed the inhibitory effect. The inhibition observed could be attributed to BrdU incorporation into DNA.

Animal experiment [2]:

Animal models

Pregnant NMRI mice

Dosage form

25 mg/kg (5 mg/ml dissolved in 0.1 M Tris-buffered saline, pH 7.6), i.p.; between embryonic days E11 and E17.

Application

In pregnant NMRI mice, a single pulse of 5-bromodeoxyuridine (BrdU) was injected between embryonic days E11 and E17 to label postmitotic neuroblasts. BrdU is an analog of Thy that is incorporated into the DNA of cells undergoing the S-phase and can be detected by polyclona or monoclonal antibodies. Immunoreactive nuclei were detected in the adult CNS after pulses of BrdU. BrdU could be used to map neuronal birthdates.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1] M. Durante, C. Geri, V. Nuti-Ronchi, G. Martini, E. Guillé, J. Grisvard, L. Giorgi, R. Parenti, M. Bulatti. Inhibition of Nicotiana glauca pich tissue proliferation through incorporation of 5-BrdU into SNA. Cell Differentiation, Volume 6, Issue 1, June 1977, Pages 53–63

[2] del Rio JA, Soriano E. Immunocytochemical detection of 5'-bromodeoxyuridine incorporation in the central nervous system of the mouse. Brain Res Dev Brain Res. 1989 Oct 1;49(2):311-7.

Background

5-Bromodeoxyuridine (5-BrdU)는 S-단계를 겪고 있는 세포의 DNA에 흡수되는 Thy의 아날로그이며, monoclonal 항체 또는 polyclona111을 통해 감지될 수 있습니다.

In vitro: 니코티아나 글라우카 식물 줄기 조직의 분화 초기 단계에서 5-BrdU의 증식 효과가 연구되었습니다. 이것은 문화 첫 72시간 동안만 완전히 억제되며, 동시에 데옥시에티딘 또는 티미딘을 첨가하면 이 억제 작용이 반대로 전환됩니다 [1].

생체 내: 어린 성인 쥐의 자손들은 단일 항체와 페루옥사제-항페루옥사제 방법을 사용하여 5-BrdU로 염색되었습니다. 면역 반응성 핵의 분포는 이전 [3H] 티미딘 데이터와 매우 유사합니다. 다양한 태아 시기에서 라벨이 지정된 핵의 패턴은 대뇌 피질 및 해마 신경 생성에 대해 기술된 공간적 및 시간적 그래디언트를 따랐습니다. 이러한 데이터는 BrdU가 신경 세포 출생 날짜를 매핑하는 데 사용될 수 있음을 나타냅니다. [2].

임상 시험: 현재 임상 데이터는 없습니다.

References:
[1] M.  Durante, C. Geri, V. Nuti-Ronchi, G. Martini, E. Guillé, J. Grisvard, L. Giorgi, R. Parenti, M. Bulatti. Inhibition of Nicotiana glauca pich tissue proliferation through incorporation of 5-BrdU into SNA. Cell Differentiation, Volume 6, Issue 1, June 1977, Pages 53–63
[2] del Rio JA, Soriano E.   Immunocytochemical detection of 5'-bromodeoxyuridine incorporation in the central nervous system of the mouse. Brain Res Dev Brain Res. 1989 Oct 1;49(2):311-7.

Chemical Properties

Cas No. 59-14-3 SDF
Synonyms BrdU, Bromodeoxyuridine, Broxuridine, NSC 38297
Chemical Name 5-bromo-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione
Canonical SMILES BrC(C(N1)=O)=CN(C1=O)[C@@]2([H])C[C@@](O)([H])[C@@](O2)([H])CO
Formula C9H11BrN2O5 M.Wt 307.1
Solubility ≥ 15.35mg/mL in DMSO, ≥ 16.23 mg/mL in Water with ultrasonic, ≥ 11.56 mg/mL in 0.9% NS with ultrasonic Storage 4°C, protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 3.2563 mL 16.2813 mL 32.5627 mL
5 mM 0.6513 mL 3.2563 mL 6.5125 mL
10 mM 0.3256 mL 1.6281 mL 3.2563 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

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Related Video

    5-BrdU-GlpBio

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