8-OH-DPAT (8-Hydroxy-DPAT) (Synonyms: 8-Hydroxy-DPAT) |
| Catalog No.GC30888 |
8-OH-DPAT (8-Hydroxy-DPAT)은 pIC50 8.19에서 5-HT1A 수용체에 대한 강력하고 특이적인 자극활동을 나타내고 높은 선택성을 나타낸다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 78950-78-4
Sample solution is provided at 25 µL, 10mM.
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8-OH-DPAT (8-Hydroxy-DPAT)은 pIC50 8.19에서 5-HT1A 수용체에 대한 강력하고 특이적인 자극활동을 나타내고 높은 선택성을 나타낸다. 그러나 8-OH-DPAT은 5-HT1B 수용체에 대한 결합친화력이 약함(pIC50, 5.42)을 보이고 5-HT 수용체에 대한 결합은 최소적(pIC50)이다.
1-100µM의 농도 범위에서 8-OH-DPAT을 사전 처리하면 H2O2가 유도한 뉴런 세포 사망을 현저하게 억제한다[3]. 8-OH-DPAT(10 µM; 24 시간)은 배양된 RPE 세포에서 줄 lipofuscin 쌓임을 줄이다[4].
8-OH-DPAT(0-0.1 mg/kg; s.c.; 2시간)을 투여하고 젖은 묻을 제공한 쥐에서는 식사 및 활동 빈도가 증가했고 비의무 조건하에 휴식 및 총 정리 행동이 억제됐다[5]. 8-OH-DPAT(0.5 mg/kg; s.c)는 QUIN(agonist quinpirole)과 HAL(강력 한 D ₂ 할로 페리 돌 악역)의 유충 붕괴 및 유충 선호의 방해를 강화하고 HAL에 의해 유도된 쥐에서 유충 위를 돌아다니는 행동 증가를 반전시켰다[6]. 8-OH-DPAT(0.2 및 0.4mg/kg i.p.)는 상황적 조건의 경쟁만 줄여주었고 분리된 조건의 신호는 줄이지 않았다[7]. 전신 투여된 8-OH-DPAT(0.4 mg/kg)는 체내 미세투석에 의해 측정된 MPOA (미디알 전시구역)의 세포외 5-HT 수준을 낮추었다[8].
References:
[1]. Middlemiss DN, Fozard JR. 8-Hydroxy-2-(di-n-propylamino)-tetralin discriminates between subtypes of the 5-HT1 recognition site. Eur J Pharmacol. 1983 May 20;90(1):151-3. doi: 10.1016/0014-2999(83)90230-3. PMID: 6223827.
[2]. Bard JA, Zgombick J, et,al. Cloning of a novel human serotonin receptor (5-HT7) positively linked to adenylate cyclase. J Biol Chem. 1993 Nov 5;268(31):23422-6. PMID: 8226867.
[3]. Lee HJ, Ban JY, et,al. Stimulation of 5-HT1A receptor with 8-OH-DPAT inhibits hydrogen peroxide-induced neurotoxicity in cultured rat cortical cells. Pharmacol Res. 2005 Mar;51(3):261-8. doi: 10.1016/j.phrs.2004.09.003. PMID: 15661577.
[4]. Thampi P, Rao HV, et,al. The 5HT1a receptor agonist 8-Oh DPAT induces protection from lipofuscin accumulation and oxidative stress in the retinal pigment epithelium. PLoS One. 2012;7(4):e34468. doi: 10.1371/journal.pone.0034468. Epub 2012 Apr 3. PMID: 22509307; PMCID: PMC3317995.
[5]. Hartley JE, Montgomery AM. 8-OH-DPAT inhibits both prandial and waterspray-induced grooming. J Psychopharmacol. 2008 Sep;22(7):746-52. doi: 10.1177/0269881107082903. Epub 2008 Feb 28. PMID: 18308782.
[6]. Cai Y, Zhang X, et,al. 8-OH-DPAT enhances dopamine D2-induced maternal disruption in rats. J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2022 Jul;208(4):467-477. doi: 10.1007/s00359-022-01551-4. Epub 2022 Apr 17. PMID: 35434766.
[7]. Cassaday HJ, Thur KE. Intraperitoneal 8-OH-DPAT reduces competition from contextual but not discrete conditioning cues. Pharmacol Biochem Behav. 2019 Dec;187:172797. doi: 10.1016/j.pbb.2019.172797. Epub 2019 Oct 24. PMID: 31669833; PMCID: PMC6899499.
[8]. Lorrain DS, Matuszewich L, et,al. 8-OH-DPAT influences extracellular levels of serotonin and dopamine in the medial preoptic area of male rats. Brain Res. 1998 Apr 20;790(1-2):217-23. doi: 10.1016/s0006-8993(98)00065-1. PMID: 9593901.
| 세포 실험 [1]: | |
세포 라인 | 망막색소상피세포 (RPE) |
반응 조건 | 10 µM;24 시간 동안 |
응용 분야 | 8-OH-DPAT은 배양된 RPE 세포에서 lipofuscin 쌓임을 줄입니다. |
| 동물 실험 [2]: | |
동물 모형 | 수컷 sprague-dawley 쥐 |
제조 방법 | 8-OH-DPAT(0-0.1 mg/kg)의 전후거동에에 대한 투여-반응관계가 연구되었으므로 8-OH-DPAT이 수정 행동의 다양한 면에 미치는 영향을 결정합니다. |
제형 | 0-0.1 mg/kg; s.c.;2시간 |
응용 분야 | 8-OH-DPAT을 투여받고 젖은 묻을 제공받은 쥐에서는 식사 및 활동 빈도가 증가했지만 비의무 조건하에 휴식 및 총 정리 행동이 억제됐습니다. |
참고문헌: [1]. Thampi P, Rao HV, et,al. The 5HT1a receptor agonist 8-OH-DPAT induces protection from lipofuscin accumulation and oxidative stress in the retinal pigment epithelium. PLoS One. 2012;7(4):e34468. doi: 10.1371/journal.pone.0034468. Epub 2012 Apr 3. PMID: 22509307; PMCID: PMC3317995.[2]. Hartley JE, Montgomery AM. 8-OH-DPAT inhibits both prandial and waterspray-induced grooming. J Psychopharmacol. 2008 Sep;22(7):746-52. doi: 10.1177/0269881107082903. Epub 2008 Feb 28. PMID: 18308782. | |
| Cas No. | 78950-78-4 | SDF | |
| Synonyms | 8-Hydroxy-DPAT | ||
| Canonical SMILES | OC1=C2CC(N(CCC)CCC)CCC2=CC=C1 | ||
| Formula | C16H25NO | M.Wt | 247.38 |
| Solubility | 150mg/ml in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 4.0424 mL | 20.2118 mL | 40.4236 mL |
| 5 mM | 0.8085 mL | 4.0424 mL | 8.0847 mL |
| 10 mM | 0.4042 mL | 2.0212 mL | 4.0424 mL |
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- Purity: >95.00%
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