>>Peptides>>β-amyloid (12-28) (TFA)

β-amyloid (12-28) (TFA) (Synonyms: Amyloid β-Protein (12-28) (TFA); Amyloid Beta-Peptide (12-28) (human) TFA; β-Amyloid protein fragment(12-28) TFA)

Catalog No.GC61988

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β-amyloid (12-28) (TFA) Chemical Structure

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1 mg
US$108.00
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5 mg
US$270.00
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

β-amyloid (12-28) TFA is a peptide fragment of β-amyloid protein (β1-42). β1-42 a 42 amino acid protein , is the major component of senile plaque cores. β-amyloid (12-28) shows aggregation properties. β-amyloid (12-28) has the potential for Alzheimer’s disease research[1].

β-amyloid (12-28) may exert dysregulation cognitive effects by means of defective coordination of potassium channel function in nerve, glia and endothelial cells[1].β-Amyloid Aggregation Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1. Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure.2. The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 ℃.3. The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing.4. Next, the solution was age 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments.Methods vary depends on the downstream applications.

[1]. Rabanal F, et al. Structural, kinetic and cytotoxicity aspects of 12-28 beta-amyloid protein fragment: a reappraisal. J Pept Sci. 2002 Oct;8(10):578-88.
[2]. Nikunj S Patel, et al. Potent anti-angiogenic motifs within the Alzheimer beta-amyloid peptide. Amyloid. 2008 Mar;15(1):5-19.

Chemical Properties

Cas No. SDF
Synonyms Amyloid β-Protein (12-28) (TFA); Amyloid Beta-Peptide (12-28) (human) TFA; β-Amyloid protein fragment(12-28) TFA
Formula C91H136N25F3O27 M.Wt 2069.2
Solubility DMSO : 120 mg/mL (57.99 mM) Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 0.4833 mL 2.4164 mL 4.8328 mL
5 mM 0.0967 mL 0.4833 mL 0.9666 mL
10 mM 0.0483 mL 0.2416 mL 0.4833 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

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Review for β-amyloid (12-28) (TFA)

Average Rating: 5 ★★★★★ (Based on Reviews and 29 reference(s) in Google Scholar.)

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