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Amyloid Beta-peptide (25-35) (human)

Catalog No.GP10082

알츠하이머 아밀로이드 베타 펩타이드의 단편인 인간 Amyloid beta-peptide (25-35)은 신경독성 효과가 있습니다.

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Amyloid Beta-peptide (25-35) (human) Chemical Structure

Cas No.: 131602-53-4

Size 가격 재고 수량
1mg
US$43.00
재고 있음
5mg
US$125.00
재고 있음
10mg
US$218.00
재고 있음
25mg
US$301.00
재고 있음

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Cell experiment [1]:

Cell lines

PC12 cells

Preparation method

Preparation of Aggregated Amyloid beta(Aβ)-peptide (25-35) (human): Amyloid beta(Aβ)-peptide (25-35) (human) was dissolved in deionized water with a concentration of 1mM, filtered and sterilized,37℃ Incubated for 4 days to induce aggregation, and stored at -20℃ for use.

Cells were seeded onto 96-well culture plate at a density of 2 ×104 cells/well. The cells were at first stabilized at 37℃ for 48 h and subsequently cultured in free serum medium and Aβ-peptide (25-35) (human) at a final concentration of 20 μM was then added to the culture for an additional 24 h.

Reaction Conditions

20 μM; 24 h

Applications

Treatment of PC12 cells with 20 μM of Amyloid beta(Aβ)-peptide (25-35) (human) for 24 h induced cytotoxicity as the cell viability was reduced to 67% of the control value (100%).

Animal experiment [2]:

Animal models

Male rats of the Sprague–Dawley

Preparation method

The flinch, vocalization and jump thresholds with electric footshock were measured, 8 days after the injection of Amyloid beta(Aβ)-peptide (25-35) (human) at the dose of 15 nmol/5 μl.

Dosage form

15 nmol/5 μl; i.c.v.;8days

Applications

Amyloid beta(Aβ)-peptide (25-35) (human) induced a marked decrease in latency in step-through passive avoidance task.

References:

[1]. Xian YF, Lin ZX, et,al. Protective effect of isorhynchophylline against β-amyloid-induced neurotoxicity in PC12 cells. Cell Mol Neurobiol. 2012 Apr;32(3):353-60. doi: 10.1007/s10571-011-9763-5. Epub 2011 Nov 1. PMID: 22042506.

[2]. Yamaguchi Y, Kawashima S. Effects of amyloid-beta-(25-35) on passive avoidance, radial-arm maze learning and choline acetyltransferase activity in the rat. Eur J Pharmacol. 2001 Feb 2;412(3):265-72. doi: 10.1016/s0014-2999(01)00730-0. PMID: 11166290.

Background

Amyloid beta(Aβ)-peptide (25-35) (human)은 알츠하이머 병 환자의 뇌에서 흔히 발견되는 알츠하이머 아밀로이드 베타 펩타이드의 단편으로, 알츠하이머 아밀로이드 플라크의 주요 구성 성분입니다[1-3]. Amyloid beta-peptide (25-35) (human)은 신경 독성 작용 때문에 Aβ의 기능적 도메인으로 간주되며, 베타 아밀로이드의 생체 활성 영역입니다[4].

완전체 아밀로이드 베타-펩타이드 (25-35) (인간) 처리(20 μM, 6시간)는 해마 신경세포의 원색 배양에서 타우 단백질 키나제 I/글리코겐 합성 키나제-3베타(TPKI/GSK-3beta)를 활성화시켰습니다 [5]. 인간 완전체 아밀로이드 베타-펩타이드 (25-35)(100nM, 1μM, 10μM 또는 30μM)는 배양된 대뇌 피질 쥐 신경세포의 생존율을 감소시켰습니다 [6]. 인간 완전체 아밀로이드 베타-펩타이드 (25-35)(10μM)는 급여 이온 전기적 응용으로 급여한 급강도 반응의 진폭을 증가시켰습니다[7]. PC12 세포에게 인간 완전체 아밀로이드 베타 - 펩타이드(25 - 35)(20 μ M,24 시간 ) 처리하면 세포 생존율은 대조군 값(100%)의67% 로 감소되어 세포독성을 유발합니다[8].

15 nmol/5 μl의 인간 Amyloid beta-peptide (25-35)을 한 번 뇌실 내 주사(i.c.v.)로 하는 것은, 쥐에게 8일 동안 투여할 경우, 쥐들이 걸음마 기반의 수동적 회피 과제에서 반응 지연 시간이 현저하게 감소되도록 유도한다[9].

References:

[1]. Nalbantoglu J. Beta-amyloid protein in Alzheimer's disease. Can J Neurol Sci. 1991 Aug;18(3 Suppl):424-7. doi: 10.1017/s0317167100032595. PMID: 1933692.
[2]. Rush DK, Aschmies S, et,al. Intracerebral beta-amyloid(25-35) produces tissue damage: is it neurotoxic? Neurobiol Aging. 1992 Sep-Oct;13(5):591-4. doi: 10.1016/0197-4580(92)90061-2. PMID: 1281289.
[3]. Mattson MP, Cheng B, et,al. beta-Amyloid peptides destabilize calcium homeostasis and render human cortical neurons vulnerable to excitotoxicity. J Neurosci. 1992 Feb;12(2):376-89. doi: 10.1523/JNEUROSCI.12-02-00376.1992. PMID: 1346802; PMCID: PMC6575616.
[4]. D'Errico, G; Vitiello, G; et,al. Interaction between Alzheimer's A(25-35) peptide and phospholipid bilayers: The role of cholesterol. Biochimica. Biophys. Acta (BBA) – Biomembr., 2008, 1778, 2710-2716.
[5]. Takashima A, Honda T, et,al. Activation of tau protein kinase I/glycogen synthase kinase-3beta by amyloid beta peptide (25-35) enhances phosphorylation of tau in hippocampal neurons. Neurosci Res. 1998 Aug;31(4):317-23. doi: 10.1016/s0168-0102(98)00061-3. PMID: 9809590.
[6]. Wang Y, Liu L, et,al. Mechanism of soluble beta-amyloid 25-35 neurotoxicity in primary cultured rat cortical neurons. Neurosci Lett. 2016 Apr 8;618:72-76. doi: 10.1016/j.neulet.2016.02.050. Epub 2016 Mar 3. PMID: 26940239.
[7]. Carette B, Poulain P, et,al. Electrophysiological effects of 25-35 amyloid-beta-protein on guinea-pig lateral septal neurons. Neurosci Lett. 1993 Mar 5;151(1):111-4. doi: 10.1016/0304-3940(93)90059-t. PMID: 8385758.
[8]. Xian YF, Lin ZX, Mao QQ, Ip SP, Su ZR, Lai XP. Protective effect of isorhynchophylline against β-amyloid-induced neurotoxicity in PC12 cells. Cell Mol Neurobiol. 2012 Apr;32(3):353-60. doi: 10.1007/s10571-011-9763-5. Epub 2011 Nov 1. PMID: 22042506.
[9]. Yamaguchi Y, Kawashima S. Effects of amyloid-beta-(25-35) on passive avoidance, radial-arm maze learning and choline acetyltransferase activity in the rat. Eur J Pharmacol. 2001 Feb 2;412(3):265-72. doi: 10.1016/s0014-2999(01)00730-0. PMID: 11166290.

Chemical Properties

Cas No. 131602-53-4 SDF
Chemical Name Amyloid?Beta-peptide (25-35) (human)
Canonical SMILES CCC(C)C(C(=O)NC(C(C)CC)C(=O)NCC(=O)NC(CC(C)C)C(=O)NC(CCSC)C(=O)O)NC(=O)C(C)NC(=O)CNC(=O)C(CCCCN)NC(=O)C(CC(=O)N)NC(=O)C(CO)NC(=O)CN
Formula C45H81N13O14S M.Wt 1060.27
Solubility ≥106mg/mL in DMSO Storage Desiccate at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 0.9432 mL 4.7158 mL 9.4316 mL
5 mM 0.1886 mL 0.9432 mL 1.8863 mL
10 mM 0.0943 mL 0.4716 mL 0.9432 mL
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