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Beta-Sitosterol (Synonyms: β-Sitosterol (purity>98%); 22,23-Dihydrostigmasterol (purity>98%)))

Catalog No.GC20142

Beta-Sitosterol is a plant sterol with multiple biological activities, including anti-inflammatory, anti-tumor, antipyretic and immunomodulatory activities.

Products are for research use only. Not for human use. We do not sell to patients.

Beta-Sitosterol Chemical Structure

Cas No.: 83-46-5

Size 가격 재고 수량
10mg
US$41.00
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25mg
US$81.00
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50mg
US$119.00
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100mg
US$182.00
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Sample solution is provided at 25 µL, 10mM.

Description of Beta-Sitosterol

Beta-Sitosterol is a plant sterol with multiple biological activities, including anti-inflammatory, anti-tumor, antipyretic and immunomodulatory activities[1, 2]. Beta-Sitosterol can inhibit the migration of vascular smooth muscle cells through the PPARG/AMPK/mTOR pathway[3].

In vitro, Beta-Sitosterol (10-160μM) treatment of MH7A cells for 12-48h inhibited cell proliferation in a concentration- and time-dependent manner, significantly reduced the expression of MMP9, HSP90AA1, SRC, EGFR and ALB genes, and significantly increased the expression of CASP3 protein[4]. Beta-Sitosterol (0.2-1mM) treatment of human liver cancer HepG2 cells for 24h induced cytotoxicity in a dose-dependent manner and induced intracellular ROS accumulation[5].

In vivo, Beta-Sitosterol (50, 100, 200mg/kg) was intraperitoneally injected into rats with paw edema and inhibited edema in a dose-dependent manner, with the inhibitory effect reaching 50-70%[6].

References:
[1] Nandi S, Nag A, Khatua S, et al. Anticancer activity and other biomedical properties of β‐sitosterol: Bridging phytochemistry and current pharmacological evidence for future translational approaches[J]. Phytotherapy Research, 2024, 38(2): 592-619.
[2] Fraile L, Crisci E, Córdoba L, et al. Immunomodulatory properties of beta-sitosterol in pig immune responses[J]. International Immunopharmacology, 2012, 13(3): 316-321.
[3] He S, He S, Chen Y, et al. Beta-sitosterol modulates the migration of vascular smooth muscle cells via the PPARG/AMPK/mTOR pathway[J]. Pharmacology, 2022, 107(9-10): 495-509.
[4] Wang X, Mao J. Systematic pharmacology-based strategy to investigate the mechanism of beta-sitosterol for the treatment of rheumarthritis[J]. Frontiers in Genetics, 2024, 15: 1507606.
[5] Ditty M J, Ezhilarasan D. β-sitosterol induces reactive oxygen species-mediated apoptosis in human hepatocellular carcinoma cell line[J]. Avicenna Journal of Phytomedicine, 2021, 11(6): 541.
[6] Paniagua-Pérez R, Flores-Mondragón G, Reyes-Legorreta C, et al. Evaluation of the anti-inflammatory capacity of beta-sitosterol in rodent assays[J]. African Journal of Traditional, Complementary and Alternative Medicines, 2017, 14(1): 123-130.

Protocol of Beta-Sitosterol

Cell experiment [1]:

Cell lines

MH7A cells

Preparation Method

The test was divided into the control group (DMSO), positive drug group (methotrexate, 80μM), and drug intervention group (Beta-Sitosterol, 10, 20, 40, 80, and 160μM, respectively). After cells were treated with different concentrations of Beta-Sitosterol, the MTT method was used to detect the proliferation of cells at 12, 24, and 48h. 

Reaction Conditions

10, 20, 40, 80, 160μM; 12, 24, 48h

Applications

Beta-Sitosterol inhibits the proliferation of MH7A cells in a concentration- and time-dependent manner.

Animal experiment [2]:

Animal models

Wistar rats

Preparation Method

Used 30 rats organized in 6 groups with 5 individuals each: a negative control group that was intra-gastrically (IG) administered with mineral oil; three other groups that were IG administered with Beta-Sitosterol (50, 100 and 200mg/kg), previously diluted in mineral oil; and finally, two more groups intraperitoneally (i.p.) administered with known anti-inflammatory drugs, in which one group was injected 200mg/kg of ibuprofen (a non-steroidal anti-inflammatory agent), and the other group was injected with 10mg/kg of prednisone (a steroidal anti-inflammatory agent). All animals were immediately injected with 0.1mL of anti-ovalbumin antibody (diluted 1:3) in the hindquarter of the plantar area of the right leg, and in the contralateral leg; rats were injected with 0.1mL of 0.9% NaCl. In a subsequent step, all animals were intravenously (i.v.) injected with a suspension of egg albumin (25mg/kg). The volume of the swollen leg was determined with a digital pletysmometer 3h after the ovoalbumin injection. The paw edema value was established by determining the difference between the volume obtained in the treated leg and in the control (left leg). For data calculation, the volume of the swollen leg was considered to represent 100% inflammation.

Dosage form

50, 100, 200mg/kg; i.p.

Applications

The three tested doses of Beta-Sitosterol were found to induce a significant, dose dependent inhibition of the edema in comparison with the result found in the control group. The inhibition was 51, 63, and 70% with 50, 100, and 200mg/kg of Beta-Sitosterol, respectively.

References:
[1]Wang X, Mao J. Systematic pharmacology-based strategy to investigate the mechanism of beta-sitosterol for the treatment of rheumarthritis[J]. Frontiers in Genetics, 2024, 15: 1507606.
[2]Paniagua-Pérez R, Flores-Mondragón G, Reyes-Legorreta C, et al. Evaluation of the anti-inflammatory capacity of beta-sitosterol in rodent assays[J]. African Journal of Traditional, Complementary and Alternative Medicines, 2017, 14(1): 123-130.

Chemical Properties of Beta-Sitosterol

Cas No. 83-46-5 SDF
Synonyms β-Sitosterol (purity>98%); 22,23-Dihydrostigmasterol (purity>98%))
Formula C29H50O M.Wt 414.71
Solubility Ethanol : 5 mg/mL (12.06 mM; ultrasonic and warming and heat to 60°C) Storage -20°C, protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of Beta-Sitosterol

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 2.4113 mL 12.0566 mL 24.1132 mL
5 mM 0.4823 mL 2.4113 mL 4.8226 mL
10 mM 0.2411 mL 1.2057 mL 2.4113 mL
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