Beta-Sitosterol (Synonyms: β-Sitosterol (purity>98%); 22,23-Dihydrostigmasterol (purity>98%))) |
Catalog No.GC20142 |
Beta-Sitosterol is a plant sterol with multiple biological activities, including anti-inflammatory, anti-tumor, antipyretic and immunomodulatory activities.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 83-46-5
Sample solution is provided at 25 µL, 10mM.
Beta-Sitosterol is a plant sterol with multiple biological activities, including anti-inflammatory, anti-tumor, antipyretic and immunomodulatory activities[1, 2]. Beta-Sitosterol can inhibit the migration of vascular smooth muscle cells through the PPARG/AMPK/mTOR pathway[3].
In vitro, Beta-Sitosterol (10-160μM) treatment of MH7A cells for 12-48h inhibited cell proliferation in a concentration- and time-dependent manner, significantly reduced the expression of MMP9, HSP90AA1, SRC, EGFR and ALB genes, and significantly increased the expression of CASP3 protein[4]. Beta-Sitosterol (0.2-1mM) treatment of human liver cancer HepG2 cells for 24h induced cytotoxicity in a dose-dependent manner and induced intracellular ROS accumulation[5].
In vivo, Beta-Sitosterol (50, 100, 200mg/kg) was intraperitoneally injected into rats with paw edema and inhibited edema in a dose-dependent manner, with the inhibitory effect reaching 50-70%[6].
References:
[1] Nandi S, Nag A, Khatua S, et al. Anticancer activity and other biomedical properties of β‐sitosterol: Bridging phytochemistry and current pharmacological evidence for future translational approaches[J]. Phytotherapy Research, 2024, 38(2): 592-619.
[2] Fraile L, Crisci E, Córdoba L, et al. Immunomodulatory properties of beta-sitosterol in pig immune responses[J]. International Immunopharmacology, 2012, 13(3): 316-321.
[3] He S, He S, Chen Y, et al. Beta-sitosterol modulates the migration of vascular smooth muscle cells via the PPARG/AMPK/mTOR pathway[J]. Pharmacology, 2022, 107(9-10): 495-509.
[4] Wang X, Mao J. Systematic pharmacology-based strategy to investigate the mechanism of beta-sitosterol for the treatment of rheumarthritis[J]. Frontiers in Genetics, 2024, 15: 1507606.
[5] Ditty M J, Ezhilarasan D. β-sitosterol induces reactive oxygen species-mediated apoptosis in human hepatocellular carcinoma cell line[J]. Avicenna Journal of Phytomedicine, 2021, 11(6): 541.
[6] Paniagua-Pérez R, Flores-Mondragón G, Reyes-Legorreta C, et al. Evaluation of the anti-inflammatory capacity of beta-sitosterol in rodent assays[J]. African Journal of Traditional, Complementary and Alternative Medicines, 2017, 14(1): 123-130.
Cell experiment [1]: | |
Cell lines | MH7A cells |
Preparation Method | The test was divided into the control group (DMSO), positive drug group (methotrexate, 80μM), and drug intervention group (Beta-Sitosterol, 10, 20, 40, 80, and 160μM, respectively). After cells were treated with different concentrations of Beta-Sitosterol, the MTT method was used to detect the proliferation of cells at 12, 24, and 48h. |
Reaction Conditions | 10, 20, 40, 80, 160μM; 12, 24, 48h |
Applications | Beta-Sitosterol inhibits the proliferation of MH7A cells in a concentration- and time-dependent manner. |
Animal experiment [2]: | |
Animal models | Wistar rats |
Preparation Method | Used 30 rats organized in 6 groups with 5 individuals each: a negative control group that was intra-gastrically (IG) administered with mineral oil; three other groups that were IG administered with Beta-Sitosterol (50, 100 and 200mg/kg), previously diluted in mineral oil; and finally, two more groups intraperitoneally (i.p.) administered with known anti-inflammatory drugs, in which one group was injected 200mg/kg of ibuprofen (a non-steroidal anti-inflammatory agent), and the other group was injected with 10mg/kg of prednisone (a steroidal anti-inflammatory agent). All animals were immediately injected with 0.1mL of anti-ovalbumin antibody (diluted 1:3) in the hindquarter of the plantar area of the right leg, and in the contralateral leg; rats were injected with 0.1mL of 0.9% NaCl. In a subsequent step, all animals were intravenously (i.v.) injected with a suspension of egg albumin (25mg/kg). The volume of the swollen leg was determined with a digital pletysmometer 3h after the ovoalbumin injection. The paw edema value was established by determining the difference between the volume obtained in the treated leg and in the control (left leg). For data calculation, the volume of the swollen leg was considered to represent 100% inflammation. |
Dosage form | 50, 100, 200mg/kg; i.p. |
Applications | The three tested doses of Beta-Sitosterol were found to induce a significant, dose dependent inhibition of the edema in comparison with the result found in the control group. The inhibition was 51, 63, and 70% with 50, 100, and 200mg/kg of Beta-Sitosterol, respectively. |
References: |
Cas No. | 83-46-5 | SDF | |
Synonyms | β-Sitosterol (purity>98%); 22,23-Dihydrostigmasterol (purity>98%)) | ||
Formula | C29H50O | M.Wt | 414.71 |
Solubility | Ethanol : 5 mg/mL (12.06 mM; ultrasonic and warming and heat to 60°C) | Storage | -20°C, protect from light |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
1 mM | 2.4113 mL | 12.0566 mL | 24.1132 mL |
5 mM | 0.4823 mL | 2.4113 mL | 4.8226 mL |
10 mM | 0.2411 mL | 1.2057 mL | 2.4113 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5
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