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C11 BODIPY 581/591 Sale

Catalog No.GC40165

C11-BODIPY581/591는 지질 산화의 형광 비율 프로브입니다.

Products are for research use only. Not for human use. We do not sell to patients.

C11 BODIPY 581/591 Chemical Structure

Cas No.: 217075-36-0

Size 가격 재고 수량
1mg
US$145.00
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Sample solution is provided at 25 µL, 10mM.

Product has been cited by 70 publications

Product Documents

Quality Control & SDS

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Protocol

This plan only provides a guide, please modify it to meet your specific needs.
1. Preparation of staining solution
(1) Prepare storage solution: Dissolve C11 BODIPY 581/591 in DMSO and prepare a storage solution with a concentration of 1-10mM.
Note: Unused storage solution should be aliquoted and stored in the dark at -20°C or -80°C to avoid repeated freezing and thawing.
(2) Prepare working solution: Dilute the storage solution with a suitable buffer (such as serum-free medium or PBS) and prepare a working solution with a concentration of 1-10μM.
Note: Please adjust the concentration of the working fluid according to the actual situation and prepare it now.

2. Cell suspension staining
(1) Suspension cells: Centrifuge at 1000g for 3-5 minutes at 4°C, discard the supernatant, and wash twice with PBS for 5 minutes each time.
(2) Adherent cells: Wash twice with PBS, add trypsin to digest the cells, and centrifuge at 1000g for 3-5 minutes after digestion is completed.
(3) Add 1 mL of C11 BODIPY 581/591 working solution to resuspend the cells, and incubate at 37 °C in the dark for 5-30 minutes. The optimal incubation time for different cells is different, please explore by yourself according to the specific experimental needs.
(4) After the incubation, centrifuge at 1000g for 5 minutes, remove the supernatant, add PBS and wash 2-3 times, 5 minutes each time.
(5) Resuspend cells in pre-warmed serum-free cell culture medium or PBS and observe by fluorescence microscope or flow cytometry.

3. Cell adhesion staining
(1) Culture adherent cells on sterile coverslips.
(2) Remove the coverslip from the culture medium, suck out the excess culture medium, and place the coverslip in a humid environment.
(3) Add 100 μL of dye working solution from one corner of the coverslip and shake gently to evenly cover all cells with the dye.
(4) Incubate at 37 °C in the dark for 5-30 minutes. The optimal incubation time for different cells is different, please explore by yourself according to the specific experimental needs.
(5) After the incubation, discard the dye working solution and use pre-warmed culture solution to wash the coverslip 2 to 3 times.
4. Microscope detection: The excitation light and emission light of the oxidized form of C11 BODIPY 581/591 are 460-495nm and 510-550nm respectively; the excitation light and emission light of the reduced form are 565-581nm and 585-591nm respectively.
Precautions:
1) Fluorescent dyes all have quenching problems. Please avoid light as much as possible to slow down fluorescence quenching.
2) For your safety and health, please wear a lab coat and disposable gloves.

Background

C11-BODIPY581/591는 산화에 민감한 형광 지방산 유사체로, 가시 스펙트럼의 빨간색 범위에서 형광 특성을 가지고 있으며 (발광 최대값 595nm), 형광 현미경에서의 응용이 가능합니다. C11-BODIPY581/591는 막 내부에 쉽게 포함되며, 완전한 상태에서는 빨간색으로 발광하지만 자유 기호 인자로 인해 초록색으로 변합니다. 이러한 특징은 산화 활동의 비율 이미징을 (세포) 수준에서 실현할 수 있어 매우 유리합니다. 게다가 C11-BODIPY581/591의 형광 특성은 다중 웰 / 형광 판독기 접근법을 사용하여 생체 내 및 모델링된 막 내 항산화제를 고속 및 중간 처리 검사하는 것이 가능하도록 합니다.[1][2].

C11-BODIPY581/591의 최대 자극 및 방출 파장은 각각 581nm과 591nm에 해당합니다. 지질 산화의 초기체로서 CumOOH/hemin을 첨가하면, 자극 및 방출 스펙트럼이 녹색 형광 (최고 자극 파장 500 nm, 방출 파장 510 nm)에 해당하는 짧은 파장으로 이동됩니다. C11-BODIPY581/591는 수소 과산화물/Fe2+ 및 2,2'-아조비스와 같은 다른 하이드록시-, 페루옥시- 및 옥시-라디칼 생성 시스템에서도 쉽게 산화됩니다. 그러나 이 프로브는 일산화질소와 초산화물을 생성하는 SIN-1에 상대적으로 민감하지 않습니다.[3]

References:
[1]. Drummen GP, et al. C11-BODIPY581/591, an oxidation-sensitive fluorescent lipid peroxidation probe: (micro)spectroscopic characterization and validation of methodology. Free Radic Biol Med. 2002 Aug 15;33(4):473-90.
[2]. Partyka A, et al. Detection of lipid peroxidation in frozen-thawed avian spermatozoa using C11-BODIPY581/591. Theriogenology. 2011 Jun;75(9):1623-9.
[3]. Pap EH, et al. Ratio-fluorescence microscopy of lipid oxidation in living cells using C11-BODIPY581/591. FEBS Lett. 1999 Jun 25;453(3):278-82.

Chemical Properties

Cas No. 217075-36-0 SDF
Chemical Name (T-4)-difluoro[5-[[5-[(1E,3E)-4-phenyl-1,3-butadien-1-yl]-2H-pyrrol-2-ylidene-κN]methyl]-1H-pyrrole-2-undecanoato(2-)-κN1]-borate(1-), monohydrogen
Canonical SMILES [F-][B+3]1([N]2=C(/C=C/C=C/C3=CC=CC=C3)C=CC2=CC4=CC=C(CCCCCCCCCCC([O-])=O)[N-]14)[F-].[H+]
Formula C30H34BF2N2O2 • H M.Wt 504.4
Solubility 30mg/ml in DMSO,Slightly soluble in Methanol Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 1.9826 mL 9.9128 mL 19.8255 mL
5 mM 0.3965 mL 1.9826 mL 3.9651 mL
10 mM 0.1983 mL 0.9913 mL 1.9826 mL
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**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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Review for C11 BODIPY 581/591

Average Rating: 5 ★★★★★ (Based on Reviews and 33 reference(s) in Google Scholar.)

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