>>Peptides>>Calcitonin Gene Related Peptide (CGRP) (83-119), rat TFA

Calcitonin Gene Related Peptide (CGRP) (83-119), rat TFA

Catalog No.GC34395

Products are for research use only. Not for human use. We do not sell to patients.

Calcitonin Gene Related Peptide (CGRP) (83-119), rat TFA Chemical Structure

Size 가격 재고 수량
500μg
US$175.00
재고 있음
1mg
US$295.00
재고 있음
5mg
US$1,103.00
재고 있음

Tel:(909) 407-4943 Email: sales@glpbio.com

고객 리뷰

Based on customer reviews.

  • GlpBio Citations

    GlpBio Citations
  • Bioactive Compounds Premium Provider

    Bioactive Compounds Premium Provider

Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

View current batch:

Protocol

Animal experiment:

Rats[3]Male Wistar rats, weighing 150-250 g, are used throughout this study. As soon as the righting reflex is lost, the initial (t=0) measurement of cheek thickness (in mm) is made with digital calipers, the intra-oral injection given and then cheek thickness measured again at 5, 15 and 30 min, 1, 2, 3, 4, 6 and 24 h, following agonists ( Calcitonin Gene Related Peptide (CGRP) (83-119), rat (TFA), etc) or saline injections. Cheek thickness in λ-carrageenan (CG)-injected animals is measured at 0, 15, 30 min, 1, 2, 3, 4, 5, 6 and 24 h. Paw thickness (in mm) is obtained similarly, using the calipers at the time points indicated for cheek oedema, with the only difference being that the animals are not anesthetized for local (intraplantar) injections[3].

References:

[1]. S. Ghatta, et al. Calcitonin gene-related peptide: Understanding its role. Educational Forum. 20.6.2004.
[2]. Eftekhari S, et al. Calcitonin gene-related peptide (CGRP) and its receptor components in human and rat spinal trigeminal nucleus and spinal cord at C1-level. BMC Neurosci. 2011 Nov 10;12:112.
[3]. Queiroz BFG, et al. Calcitonin-gene related peptide is a potent inducer of oedema in rat orofacial tissue. Neuropeptides. 2018 Apr;68:43-48.

Background

Calcitonin Gene Related Peptide (CGRP) (83-119), rat (TFA) is a 37 amino acid calcitonin family of neuropeptide, acts through calcitonin receptor-like receptor (CRLR).

Calcitonin Gene Related Peptide (CGRP) (83-119), rat (TFA) belongs to the calcitonin family of neuropeptides which also includes adrenomedullin, amylin, calcitonin, intermedin and calcitonin receptor-stimulating peptide. Calcitonin Gene Related Peptide (CGRP) has two isoforms (αCGRP and βCGRP), and acts through calcitonin receptor-like receptor (CRLR)[1]. Calcitonin Gene Related Peptide (CGRP) plays a key role in migraine pathophysiology and is associated with activation of the trigeminovascular system. CGRP prossibly acts postjunctionally in these areas putatively involved in primary headaches[2].

Calcitonin Gene Related Peptide (CGRP) (83-119), rat (TFA) is a potent inducer of oedema in rat orofacial tissue. CGRP (100 μL; 8-33 pM) induces a rapidly developing (5-15?min) and long-lasting (6?h), dose-dependent oedema in the rat cheek. CGRP induces a smaller oedematogenic effect in the rat hind paw also blocked by the CGRP antagonist. CGRP (16 pM) potentiates the oedema induced by co-injected substance P (3.7 nM)[3].

[1]. S. Ghatta, et al. Calcitonin gene-related peptide: Understanding its role. Educational Forum. 20.6.2004. [2]. Eftekhari S, et al. Calcitonin gene-related peptide (CGRP) and its receptor components in human and rat spinal trigeminal nucleus and spinal cord at C1-level. BMC Neurosci. 2011 Nov 10;12:112. [3]. Queiroz BFG, et al. Calcitonin-gene related peptide is a potent inducer of oedema in rat orofacial tissue. Neuropeptides. 2018 Apr;68:43-48.

Chemical Properties

Cas No. SDF
Canonical SMILES Ser-Cys-Asn-Thr-Ala-Thr-Cys-Val-Thr-His-Arg-Leu-Ala-Gly-Leu-Leu-Ser-Arg-Ser-Gly-Gly-Val-Val-Lys-Asp-Asn-Phe-Val-Pro-Thr-Asn-Val-Gly-Ser-Glu-Ala-Phe-NH2 (Disulfide bridge: Cys2-Cys7)
Formula C162H262N50O52S2.C2HF3O2 M.Wt 3920.32
Solubility Water: 10 mg/mL (2.55 mM) Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 0.2551 mL 1.2754 mL 2.5508 mL
5 mM 0.051 mL 0.2551 mL 0.5102 mL
10 mM 0.0255 mL 0.1275 mL 0.2551 mL
  • Molarity Calculator

  • Dilution Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).

Calculate

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

리뷰

Review for Calcitonin Gene Related Peptide (CGRP) (83-119), rat TFA

Average Rating: 5 ★★★★★ (Based on Reviews and 33 reference(s) in Google Scholar.)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%
Review for Calcitonin Gene Related Peptide (CGRP) (83-119), rat TFA

GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.

Required fields are marked with *

You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.