>>Signaling Pathways>> Immunology/Inflammation>> Immunosuppressants>>7-hydroxy Methotrexate

7-hydroxy Methotrexate (Synonyms: NSC 380962, 7-hydroxy MTX)

Catalog No.GC49051

7-하이드록시 메토트렉세이트는 메토트렉세이트의 주요 대사 산물입니다.

Products are for research use only. Not for human use. We do not sell to patients.

7-hydroxy Methotrexate Chemical Structure

Cas No.: 5939-37-7

Size 가격 재고 수량
500 µg
US$153.00
재고 있음
1 mg
US$290.00
재고 있음
5 mg
US$1,144.00
재고 있음
10 mg
US$1,677.00
재고 있음

Tel:(909) 407-4943 Email: sales@glpbio.com

고객 리뷰

Based on customer reviews.

  • GlpBio Citations

    GlpBio Citations
  • Bioactive Compounds Premium Provider

    Bioactive Compounds Premium Provider

Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

View current batch:

Background

7-hydroxy Methotrexate (7-hydroxy MTX) is the major metabolite of the folic acid derivative MTX .1 It is formed by the 7-oxidation of MTX by hepatic aldehyde oxidases. Unlike MTX, 7-hydroxy MTX does not inhibit thymidylate synthase in isolated human acute myeloid and acute lymphoblastic leukemia cells.2 7-hydroxy MTX has a maximum tolerated dose of 0.1 g/kg, which is 30- to 50-fold lower than MTX, in rats.3 Serum levels of 7-hydroxy MTX have been used as a marker of MTX toxicity during chemotherapy.4,5

1.Johns, D.G., Iannotti, A.T., Sartorelli, A.C., et al.The relative toxicites of methotrexate and aminopterinBiochem. Pharmacol.15(5)555-561(1966) 2.Lingg, R.M., Hempel, G., Rots, M.G., et al.Effects and interaction of 7-hydroxy methotrexate and methotrexate in leukaemic cells ex vivo measured by the thymidylate synthase inhibition assayCancer Chemother. Pharmacol.56(3)322-327(2005) 3.FuskevÅg, O.M., Kristiansen, C., Lindal, S., et al.Maximum tolerated doses of methotrexate and 7-hydroxy-methotrexate in a model of acute toxicity in ratsCancer Chemother. Pharmacol.46(1)69-73(2000) 4.Schofield, R.C., Ramanathan, L.V., Murata, K., et al.Development of an assay for methotrexate and its metabolites 7-hydroxy methotrexate and DAMPA in serum by LC-MS/MSMethods in Molecular Biology213-222(2016) 5.Klapkova, E., Kukacka, J., Kotaska, K., et al.The influence of 7-OH methotrexate metabolite on clinical relevance of methotrexate determinationClin. Lab.57(7-8)599-606(2011)

Chemical Properties

Cas No. 5939-37-7 SDF
Synonyms NSC 380962, 7-hydroxy MTX
Canonical SMILES NC1=C2C(NC(C(CN(C3=CC=C(C=C3)C(N[C@H](C(O)=O)CCC(O)=O)=O)C)=N2)=O)=NC(N)=N1
Formula C20H22N8O6 M.Wt 470.4
Solubility DMSO: slightly soluble,Ethanol: slightly soluble Storage -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 2.1259 mL 10.6293 mL 21.2585 mL
5 mM 0.4252 mL 2.1259 mL 4.2517 mL
10 mM 0.2126 mL 1.0629 mL 2.1259 mL
  • Molarity Calculator

  • Dilution Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).

Calculate

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

리뷰

Review for 7-hydroxy Methotrexate

Average Rating: 5 ★★★★★ (Based on Reviews and 15 reference(s) in Google Scholar.)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%
Review for 7-hydroxy Methotrexate

GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.

Required fields are marked with *

You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.