>>Analytical Standards>>(E/Z)-4-hydroxy Tamoxifen-d5

(E/Z)-4-hydroxy Tamoxifen-d5 (Synonyms: Afimoxifene-d5, 4-OHT-d5)

Catalog No.GC49189

An internal standard for the quantification of (E/Z)-4-hydroxy tamoxifen

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(E/Z)-4-hydroxy Tamoxifen-d5 Chemical Structure

Cas No.: N/A

Size 가격 재고 수량
500 µg
US$248.00
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1 mg
US$473.00
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

(E/Z)-4-hydroxy Tamoxifen-d5 is intended for use as an internal standard for the quantification of (E/Z)-4-hydroxy tamoxifen by GC- or LC-MS. (E/Z)-4-hydroxy Tamoxifen is an active metabolite of tamoxifen .1 It is formed from tamoxifen by the cytochrome P450 (CYP) isoform CYP2D6. (E/Z)-4-hydroxy Tamoxifen is cytotoxic to MCF-7 and MDA-MB-231 breast cancer cells (IC50s = 27 and 18 µM, respectively).2 It also stimulates LC3 lipidation and the formation of autophagic vesicles in MCF-7 cells in a superoxide-dependent manner.3

1.Desta, Z., Ward, B.A., Soukhova, N.V., et al.Comprehensive evaluation of tamoxifen sequential biotransformation by the human cytochrome P450 system in vitro: Prominent roles for CYP3A and CYP2D6J. Pharmacol. Exp. Ther.310(3)1062-1075(2004) 2.Seeger, H., Huober, J., Wallwiener, D., et al.Inhibition of human breast cancer cell proliferation with estradiol metabolites is as effective as with tamoxifenHorm. Metab. Res.36(5)277-280(2004) 3.Duan, L., Danzer, B., Levenson, V.V., et al.Critical roles for nitric oxide and ERK in the completion of prosurvival autophagy in 4OHTAM-treated estrogen receptor-positive breast cancer cellsCancer Lett.353(2)290-300(2014)

Chemical Properties

Cas No. N/A SDF
Synonyms Afimoxifene-d5, 4-OHT-d5
Canonical SMILES OC1=CC=C(C(C2=CC=C(OCCN(C)C)C=C2)=C(C3=CC=CC=C3)C([2H])([2H])C([2H])([2H])[2H])C=C1
Formula C26H24D5NO2 M.Wt 392.6
Solubility DMSO: soluble,Methanol: soluble Storage -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 2.5471 mL 12.7356 mL 25.4712 mL
5 mM 0.5094 mL 2.5471 mL 5.0942 mL
10 mM 0.2547 mL 1.2736 mL 2.5471 mL
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