Ceruletide (Caerulein) |
Catalog No.GC30008 |
세룰레티드 (카에루레인)은 10개의 아미노산으로 이루어진 강력한 담낭축삭 호르몬 수용체 작용제입니다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 17650-98-5
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment [1]: | |
Cell lines |
Isolated rat pancreatic glands |
Preparation Method |
An isolated external perfusion of a rat pancreas included a normal perfusion (KRB, 60 minutes), a long term perfusion (KRB, 240 minutes) and a perfusion (60 minutes) including an additive of the detergents triton x-100 or the cholecystokinin analogue ceruletide (1x10-8 M). |
Reaction Conditions |
1x10-8 M; 60 or 240 min |
Applications |
During a perfusion with the cholecystokinin analogue ceruletide (1x108 M), there is an increase of lipase after 30 minutes and an increase of amylase after 50 minutes perfusion. |
Animal experiment [2]: | |
Animal models |
Rats |
Preparation Method |
Rats were i.v. infused for 6 h with either ceruletide (5 μg/kg/h) or ceruletide + Gabexate mesilate (50 mg/kg/h). |
Dosage form |
5 μg/kg/h; i.v. |
Applications |
In Gabexate mesilate-treated rats the serum amylase and trypsinogen concentrations were reduced by 60 and 80%, respectively, compared to rats infused with ceruletide alone. |
References: [1]. Mantke R, et al. Die isolierte extrakorporale Perfusion des Rattenpankreas - Ein Modell zur Untersuchung der Pathophysiologie der akuten Pankreatitis [The isolated perfused rat pancreas - an experimental model for investigation the early events in the pathogenesis of acute pancreatitis]. Zentralbl Chir. 2001 Nov;126(11):929-33. German. [2]. Wisner JR Jr, et al. Gabexate mesilate (FOY) protects against ceruletide-induced acute pancreatitis in the rat. Pancreas. 1987;2(2):181-6. |
세룰레티드는 10개의 아미노산으로 이루어진 강력한 담낭축삭제이며, 인간과 동물에서 담낭 근육 및 쓸개에 직접적인 경련작용을 가지고 있습니다. [1]
체외 실험에서는 1, 10 및 100 nmol/L 농도에서 cerulein이 NF-kB 결합 활성을 용량 의존적으로 유발하는 것으로 나타났습니다. 그러나 0.3 nmol/L의 cerulein은 NF-kappaB/Rel 활성화에 영향을 주지 않았습니다.[7]
체룰레티드 100 μg/kg 용량으로 생체 내에서, 셰임 수술 및 뇌간 절제된 마우스에서 자발적인 활동과 일어서는 속도를 감소시키고, 메틸페니데이트 및 메탐페타민 유도 과잉 활동을 억제하는 것이 동일하게 나타났다.[2] 토끼에 대한 생체 내 유효성 실험 결과, 체룰레티드 8 및 50 μg/kg 처리 시 혈장 호모바닐릭산 수준이 감소되었지만 크게 차이가 없었다. 그러나 체룰레티드 140 및 200 μg/kg 처리 시 혈장 호모바닐릭산은 현저하게 감소하였다.[3] 체룰레티드 (100 μg/kg, s.c.)는 중추 도파민계에 영향을 주며 신경약리제의 중추 효과를 증대시켜 임상 연구에서 강력한 치료 효과를 보였다.[4] 마우스 저산소 모델에서 피하로 1-100 μg/kg 체룰레티드 처리는 성능 저하를 예방하였으며, 개선은 저산소의 심각도와 관련이 있었다.[5] 또한 마우스에서 이산화탄소 회피 과제에서 복막 내 10-300 μg/kg 체룰레티드 처리는 반응률(셔틀 빈도)을 약간 감소시켰다.[6]
References:
[1].Vincent ME, et al. Pharmacology, clinical uses, and adverse effects of ceruletide, a cholecystokinetic agent. Pharmacotherapy. 1982 Jul-Aug;2(4):223-34.
[2].Moroji T, Hagino Y. Bilateral subdiaphragmatic vagotomy does not prevent the behavioral effects of systematically administered ceruletide in mice. Neuropeptides. 1987 Apr;9(3):217-24.
[3].Wakata N, et al. Effect of ceruletide on plasma monoamine metabolites in the rabbit. J Neurol Sci. 1991 May;103(1):97-100
[4].Hagino Y, Moroji T. Effect of ceruletide on discriminated avoidance behavior in rats. Neuropeptides. 1987 Nov-Dec;10(4):335-42.
[5].Maurice T, et al. Cholecystokinin-related peptides, after systemic or central administration, prevent carbon monoxide-induced amnesia in mice. J Pharmacol Exp Ther. 1994 May;269(2):665-73.
[6].Kuribara H, et al. Effects of ceruletide, administered singly and in combination with central-acting drugs, on discrete shuttle avoidance response in mice. Jpn J Pharmacol. 1990 Nov;54(3):325-9.
[7].Steinle AU, et al. NF-kappaB/Rel activation in cerulein pancreatitis. Gastroenterology. 1999 Feb;116(2):420-30.
Cas No. | 17650-98-5 | SDF | |
Canonical SMILES | {pGlu}-Gln-Asp-Tyr(SO3H)-Thr-Gly-Trp-Met-Asp-Phe-NH2 | ||
Formula | C58H73N13O21S2 | M.Wt | 1352.41 |
Solubility | Water : ≥ 100 mg/mL (73.94 mM);DMSO : ≥ 100 mg/mL (73.94 mM) | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 0.7394 mL | 3.6971 mL | 7.3942 mL |
5 mM | 0.1479 mL | 0.7394 mL | 1.4788 mL |
10 mM | 0.0739 mL | 0.3697 mL | 0.7394 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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