>>Natural Products>>Diphenylterazine (DTZ)

Diphenylterazine (DTZ) (Synonyms: DTZ)

Catalog No.GC33428

디페닐테라진(DTZ)은 생물 발광제입니다.

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Diphenylterazine (DTZ) Chemical Structure

Cas No.: 344940-63-2

Size 가격 재고 수량
1mg
US$105.00
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5mg
US$210.00
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10mg
US$329.00
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50mg
US$686.00
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Cell experiment [1]:

Cell lines

HEK 293T

Preparation Method

Cell viability was determined using RealTime-Glo MT Cell Viability Assay (Promega) after incubation of HEK 293T cells with individual luciferin substrates for 24 h at 37 °C. Cell morphology was further evaluated using microscopy.

Reaction Conditions

24h 30 μM Diphenylterazine

Applications

Compared to other substrates,As a novel luciferase substrate based on CTZ analogue, Diphenylterazine has bright red-shift bioluminescence. Moreover, Diphenylterazine alone yielded very little background and thus exhibited excellent signal-to-background ratios , Furthermore, Diphenylterazine elicited minimal cell toxicity at millimolar concentrations.

Animal experiment [2]:

Animal models

BALB/c mice

Preparation Method

Bioluminescence imaging of intravenously injected HEK 293T cells:BALB/c mice are used and transfected with cells expressing biotinase gene by injecting cells into the tail vein of BALB/c mice. After the diminishing of the bioluminescence, 0.3 µmol Diphenylterazine is intraperitoneally injected. Mice are imaged with a 1-min exposure per frame over a course of 20 min.

Dosage form

0.3 µmol Diphenylterazine

Applications

Diphenylterazine was used as an emerging luciferase reporter substrate in mice. The imaging of deep tissue targets in mice was evaluated by injecting luciferase gene expressing HEK 293T cells through the tail vein. First, Diphenylterazine as a substrate has no background in control mice, and when injected into mice transfected with the luciferase gene, the fluorescence emitted by Diphenylterazine can be detected, and the intensity of Diphenylterazine has increased significantly compared with other substrates.

References:

[1]. Yeh HW, Karmach O, Ji A, Carter D, Martins-Green MM, Ai HW. Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging. Nat Methods. 2017 Oct;14(10):971-974. doi: 10.1038/nmeth.4400. Epub 2017 Sep 4. PMID: 28869756; PMCID: PMC5678970.

Background

디페닐테라진은 CTZ의 아날로그로, 루시페라아제 기질로 사용하기 좋습니다. 디페닐테라진은 생물 발광제입니다. 디페닐테라진만으로는 매우 적은 배경을 나타내어 우수한 신호 대 배경 비율을 보여줍니다. 게다가 밀리몰 농도에서 최소한의 세포 독성을 유발합니다. 디페닐 테라진은 저렴한 상업용 시약으로부터 2 단계 합성이 가능하며 수율이 우수합니다[1].

세포 실험에서, Diphenylterazine는 다른 기질에 비해 신호 대 잡음 비율이 낮으며, 밀리몰 농도에서 최소한의 세포 독성을 유발합니다.

반면에, 다른 시험된 기질은 시험된 기질 농도 범위 내에서 세포 사멸을 유발시켰습니다.

쥐 실험에서, 표면 부위의 생물 발광은 0.1mM 하부 기질 농도에서 Diphenylterazine 밝기가 대조군보다 높았습니다.

깊은 조직 대상의 영상화에도 마찬가지였으며, BALB/c 쥐에게 Diphenylterazine 주사를 하여도 어떠한 배경 방출물도 생기지 않았고, 마우스 정맥 내주사시에도 여전히 형광이 감지됩니다[2].

References:
[1].Tian X, Zhang Y, Li X, Xiong Y, Wu T, Ai HW. A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice. Nat Commun. 2022 Jul 8;13(1):3967. doi: 10.1038/s41467-022-31673-x. PMID: 35803917; PMCID: PMC9270435.
[2]. Yeh HW, et al. Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging. Nat Methods. 2017 Oct;14(10):971-974.

Chemical Properties

Cas No. 344940-63-2 SDF
Synonyms DTZ
Canonical SMILES O=C1C(CC2=CC=CC=C2)=NC3=C(C4=CC=CC=C4)NC(C5=CC=CC=C5)=CN31
Formula C25H19N3O M.Wt 377.44
Solubility DMF : 11 mg/mL Storage Store at -20°C, protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 2.6494 mL 13.2471 mL 26.4943 mL
5 mM 0.5299 mL 2.6494 mL 5.2989 mL
10 mM 0.2649 mL 1.3247 mL 2.6494 mL
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Review for Diphenylterazine (DTZ)

Average Rating: 5 ★★★★★ (Based on Reviews and 4 reference(s) in Google Scholar.)

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