(-)-Epigallocatechin gallate (EGCG) (Synonyms: EGCG) |
| Catalog No.GC14049 |
(-)-Epigallocatechin gallate (EGCG)은 녹차의 주요 폴리페놀로 세포 증식을 억제하고 조사소를 유도한다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 989-51-5
Sample solution is provided at 25 µL, 10mM.
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(-)-Epigallocatechin gallate (EGCG)은 녹차의 주요 폴리페놀로 세포 증식을 억제하고 조사소를 유도한다. EGCG는 자유 기질 제거 특성을 가지고 있다[3]. EGCG는 신경 세포의 산화스트레스에 대한 항염색 및 항조사 효과를 가지고 있다[4]. 게다가 글루탐산 탈수소효소 1/2 (GDH1/2, GLUD1/2)의 활동을 억제한다[5].
(-)-Epigallocatechin Gallate (EGCG) (200 µM; 1시간)은 CoCl2 처리된 BV2 세포에서 인플레임마토리 싸이토킴 및 매개체의 표현 수준을 낮춘다[6]. EGCG는 miR-143/MAPK7 경로를 통해 3T3-L1 세포 성장을 억제한다[7].
(-)-Epigallocatechin gallate (EGCG) (5-30 mg/kg; 구강투여; 14일)는 종양(HT-29 대장암) 성장을 줄인다[8]. EGCG 치료(매일 50mg/kg)는 옥수수 기름에 녹여 복강 주사로 제7일부터 제11일까지 집 먼지 진드기(HDM) 도전 1시간 전에 투여하여 HIF-1α/VEGFA를 억제함으로써 마크로파지 M2 기울기를 조절하여 마우스의 천식 증상을 완화시킨다[9].
References:
[1]. Jin CF, Shen SR Sr, et,al. Different effects of five catechins on 6-hydroxydopamine-induced apoptosis in PC12 cells. J Agric Food Chem. 2001 Dec;49(12):6033-8. doi: 10.1021/jf010903r. PMID: 11743804.
[2]. De Amicis F, Perri A, et,al. Epigallocatechin gallate inhibits growth and epithelial-to-mesenchymal transition in human thyroid carcinoma cell lines. J Cell Physiol. 2013 Oct;228(10):2054-62. doi: 10.1002/jcp.24372. PMID: 23553645.
[3]. Kondo K, Kurihara M, et,al. Scavenging mechanisms of (-)-epigallocatechin gallate and (-)-epicatechin gallate on peroxyl radicals and formation of superoxide during the inhibitory action. Free Radic Biol Med. 1999 Oct;27(7-8):855-63. doi: 10.1016/s0891-5849(99)00133-1. PMID: 10515590.
[4]. Schroeder EK, Kelsey NA, et,al. Green tea epigallocatechin 3-gallate accumulates in mitochondria and displays a selective antiapoptotic effect against inducers of mitochondrial oxidative stress in neurons. Antioxid Redox Signal. 2009 Mar;11(3):469-80. doi: 10.1089/ars.2008.2215. PMID: 18754708.
[5]. Peeters TH, Lenting K, et,al. Isocitrate dehydrogenase 1-mutated cancers are sensitive to the green tea polyphenol epigallocatechin-3-gallate. Cancer Metab. 2019 May 20;7:4. doi: 10.1186/s40170-019-0198-7. PMID: 31139406; PMCID: PMC6526618.
[6]. Kim SR, Seong KJ, et,al. Epigallocatechin Gallate Protects against Hypoxia-Induced Inflammation in Microglia via NF-κB Suppression and Nrf-2/HO-1 Activation. Int J Mol Sci. 2022 Apr 4;23(7):4004. doi: 10.3390/ijms23074004. PMID: 35409364; PMCID: PMC8999549.
[7]. Chen CP, Su TC, et,al. Green tea epigallocatechin gallate suppresses 3T3-L1 cell growth via microRNA-143/MAPK7 pathways. Exp Biol Med (Maywood). 2022 Sep;247(18):1670-1679. doi: 10.1177/15353702221108925. Epub 2022 Jul 27. PMID: 35894140; PMCID: PMC9597208.
[8]. Jin H, Gong W, et,al. Epigallocatechin gallate inhibits the proliferation of colorectal cancer cells by regulating Notch signaling. Onco Targets Ther. 2013;6:145-53. doi: 10.2147/OTT.S40914. Epub 2013 Mar 8. PMID: 23525843; PMCID: PMC3596123.
[9]. Yang N, Li X. Epigallocatechin gallate relieves asthmatic symptoms in mice by suppressing HIF-1α/VEGFA-mediated M2 skewing of macrophages. Biochem Pharmacol. 2022 Aug;202:115112. doi: 10.1016/j.bcp.2022.115112. Epub 2022 May 29. PMID: 35640712.
| 세포 실험 [1]: | |
세포 라인 | BV2세포 |
제조 방법 | 세포는 CoCl2(350µM)에 8시간 노출하기 전에 EGCG(200µM)으로 1시간 동안 처리되었습니다. |
반응 조건 | 200 µM; 1 시간 동안 |
응용 분야 | (-)-Epigallocatechin Gallate (EGCG)는 CoCl2 처리된 BV2 세포에서 인플레임마토리 싸이토킨 및 매개체의 표현 수준을 낮춥니다. |
| 동물 실험 [2]: | |
동물 모형 | BALB/c 누드 쥐(운반형HT-29 대장암) |
제조 방법 | 치료 그룹에서는 매일 1회, 14일간 구강 내 투여로 5-20mg/kg의 EGCG를 투여했고 대조 그룹에서는 매일 1회, 14일간 구강 내 투여로 100uL의 식염수를 투여했습니다. |
제형 | 5 -30 mg/kg; i.g; 14일 |
응용 분야 | (-)-Epigallocatechin gallate (EGCG)는 종양 성장을 억제합니다. |
참고문헌: [1]. Kim SR, Seong KJ, et,al. Epigallocatechin Gallate Protects against Hypoxia-Induced Inflammation in Microglia via NF-κB Suppression and Nrf-2/HO-1 Activation. Int J Mol Sci. 2022 Apr 4;23(7):4004. doi: 10.3390/ijms23074004. PMID: 35409364; PMCID: PMC8999549.[2]. Jin H, Gong W, et,al. Epigallocatechin gallate inhibits the proliferation of colorectal cancer cells by regulating Notch signaling. Onco Targets Ther. 2013;6:145-53. doi: 10.2147/OTT.S40914. Epub 2013 Mar 8. PMID: 23525843; PMCID: PMC3596123. | |
| Cas No. | 989-51-5 | SDF | |
| Synonyms | EGCG | ||
| Chemical Name | [(2R,3R)-5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)-3,4-dihydro-2H-chromen-3-yl] 3,4,5-trihydroxybenzoate | ||
| Canonical SMILES | C1C(C(OC2=CC(=CC(=C21)O)O)C3=CC(=C(C(=C3)O)O)O)OC(=O)C4=CC(=C(C(=C4)O)O)O | ||
| Formula | C22H18O11 | M.Wt | 458.37 |
| Solubility | ≥ 22.9mg/mL in DMSO; ≥ 20mg/mL in Water | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.1816 mL | 10.9082 mL | 21.8164 mL |
| 5 mM | 0.4363 mL | 2.1816 mL | 4.3633 mL |
| 10 mM | 0.2182 mL | 1.0908 mL | 2.1816 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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