Ferrostatin-1 (Fer-1)
|
| Catalog No.GC10380 |
Ferrostatin-1은 강력한 철사병억제제로 EC50가 60nM이다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 347174-05-4
Sample solution is provided at 25 µL, 10mM.
- Free Radical Bio Med (2025).
- Phytomedicine (2025)：157269.
- Food Chem Toxicol (2025)：115590.PMID:40472920
- Phytomedicine (2025)：156843.
- Clin. Transl. Med. 15.3 (2025)：e70270.PMID:40088428
- Mol Metab (2024)：101944.PMID:38642891
- Free Radical Bio Med (2023).PMID:37805047
- Free Radical Bio Med (2024).PMID:39117049
- Ecotox Environ Safe 271 (2024)：115994.PMID:38262094
- Free Radical Bio Med (2023).PMID:38092273
- J Transl Med 22.1 (2024)：1-18.PMID:38594779
- Front Immunol 13 (2022)：967151.PMID:36341347
- Biomed Pharmacother 163 (2023)：114795.PMID:37146415
- Biomed Pharmacother (2023)：115140.PMID:37429233
- Molecules 26.4 (2021)：1092.PMID:36309080
- Biochem Pharmacol (2024)：116345.PMID:38852643
- Biochem Pharmacol 206 (2022)：115329.PMID:36309080
- Front Pharmacol 13 (2022)：1002774.PMID:36339535
- J Agr Food Chem (2025).PMID:40493028
- Front Immunol 16 (2025)：1626295.
- Int Immunopharmacol 163 (2025)：115188.PMID:40652583
- Int. Immunopharmacol. 153 (2025)：114430.PMID:40101415
- Commun Biol 8.1 (2025)：90.PMID:39833490
- Eur J Pharmacol (2024)：176528.PMID:38556118
- Front Oncol 12 (2022)：930654.PMID:36033479
- Mol Nutr Food Res (2024)：2300123.PMID:38196088
- Cell Signal 114 (2024)：111006.PMID:38086436
- Chem-Biol Interact (2023)：110828.PMID:38081571
- Eur J Pharmacol (2025)：178123.PMID:40914268
- Fish Shellfish Immun (2025)：110416.PMID:40373888
- Bba-Mol Basis Dis (2024)：167551.PMID:39437857
- J Sci Food Agric (2023).PMID:37850313
- Neurochem Res 50.3 (2025)：149.PMID:40257585
- Rsc Adv (2023)：22148-22157.PMID:37492506
- Biol 14.6 (2025)：594.
- Fish Shellfish Immun (2025)：110416.PMID:40373888
- J Sci Food Agr (2024).PMID:37850313
- Rsc Adv 14.18 (2024)：12864-12872.PMID:38650686
- Toxicol Appl Pharm (2023)：116794.PMID:38142782
- The FASEB Journal 38.14 (2024)：e23805.PMID:39003630
- Biochem Bioph Res Co 640 (2023)：183-191.PMID:36516527
- Biochem Bioph Res Co (2025)：151972.
- Renal Failure 47.1 (2025)：2548613.PMID:40887103
- Med Oncol 40.8 (2023)：230.PMID:37421513
- Exp Eye Res(2024)：110021.PMID:39117136
- Biochem Cell Biol (2023).PMID:36927169
- Free Radical Res (2022)：398-410.PMID:36194238
- Faseb J 38.15 (2024)：e23876.
- Bioengineered 12.2 (2021)：9976-9990.
- Oncol Lett 27.1 (2024)：1-11.
- Nutrients 15.22 (2023)：4845.
- Breast Cancer Res Tr 188.2 (2021)：329-342.
- bioRxiv (2023)：2023-12.
- Nat Commun 13.1 (2022)：2672.PMID:35562334jpg
- Oxid Med Cell Longev 2021 (2021)
- Nano Res (2024)：1-17.
- Free Radical Bio Med 165 (2021)：229-242.
- Oncol Lett 28.1 (2024)：1-13.
- J Reprod Immunol (2024)：104273.
- Available at SSRN 4854749.
- Burns (2024).
- Drug Resist Update (2024)：101126.
- Nano Today 35 (2020)：100981.
Ferrostatin-1은 강력한 철사병억제제로 EC50가 60nM이다.
분자 억제제로서 Ferrostatin-1은 철사병을 차단할 수 있다. Ferrostatins는 막 지질의 산화 손상을 방지함으로써 작용하는 것으로 믿어지고 있다. Ferrostatin-1은 항산화 성질을 가진 아릴알킬아민이고 철사병 억제제 중 처음으로 발견된 것들 중 하나이다. Ferrostatin-1은 erastin과 같은 작은 분자로 처리된 암세포에서 산화, 철에 의존하는 세포 사망을 완화시킨다. [3]
Ferrostatin-1은 철사병 억제제로 EC50가 60nM(HT-1080)이다. 게다가 Ferrostatin-1은 apooptosis라고 불리는 비세포사멸을 억제할 수 있다. 다파닌 선신경세포암세포(SH-SY5Y)에서 rotenone 유도된 산화적 스트레스 하에서 Ferrostatin-1의 신경 보호 역할이 보고되었습니다. Ferrostatin-1은 rotenone 자극 하에서 SH-SY5Y 세포에서 생성된 활성산소/생성가스(ROS/RNS)를 억제했다. Ferrostatin-1은 ER스트레스를 통해 세포 사멸 경로를 활성화시키는 효력이 확인되었다. 게다가 Ferrostatin-1은 rotenone 유도된 α-synuclein 집단을 완화시키는 역할도 보고되었다.[1]
Ferrostatin-1은 철사병의 특이 억제제로 헤모글로빈이 유발하는 신경세포 사망과 철 퇴적을 예방하기 위해 기관형 해마 슬라이스 문화(OHSCs)에 사용되었다. 뇌출혈(ICH) 후 Ferrostatin-1을 치료한 쥐는 두뇌 보호와 신경 기능 개선이 현저했다. 게다가 Ferrostatin-1은 지질 활성산소종의 생성을 줄이고 PTGS2 및 그 유전자제품 cyclooxygenase-2의 표현 수준을 완화시켰다. 체내 및 체외 실험에서 Ferrostatin-1은 콜라겐아제 주입 직후나 콜라겐아제 주입 후 2시간에 뇌실로 주입되었다. 뇌실 주입의 좌표는: 브레그마 기준으로 1.0mm 외측, 0.5mm 후측, 2.5mm 깊이다. [2]
References:
[1] Kabiraj P, et al. The neuroprotective role of ferrostatin-1 under rotenone-induced oxidative stress in dopaminergic neuroblastoma cells. Protein J. 2015 Oct;34(5):349-58.
[2] Li Q, et al. Inhibition of neuronal ferroptosis protects hemorrhagic brain. JCI Insight. 2017 Apr 6;2(7):e90777. doi: 10.1172/jci.insight.90777.
[3] Hofmans S, et al. Novel Ferroptosis Inhibitors with Improved Potency and ADME Properties. J Med Chem. 2016 Mar 10;59(5):2041-53.
| 세포 실험 [1]: | |
세포 라인 | SH-S5Y |
제조 방법 | DMSO (최대 100 mg/ml) 또는 에탄올 (최대 100 mg/ml)에 용해됩니다. |
반응 조건 | 1 μM, 24 시간 동안 |
응용 분야 | Ferrostatin-1은 ferroptosis라고 불리는 비세포사멸인 철사병을 억제할 수 있었습니다. Ferrostatin-1은 rotenone 유도된 산화 스트레스 하에서 다파닌 선신경세포암 세포(SH-SY5Y)에서 신경 보호 작용을 하는 것이 보고되었습니다. 게다가 Ferrostatin-1은 rotenone 자극 하에서 SH-SY5Y 세포에서 생성된 ROS/RNS를 억제하는 것이 확인되었습니다. |
| 동물 실험 [2]: | |
동물 모형 | C57BL/6 (ICH, 뇌내출혈) |
제조 방법 | 1에서 10 μ M Ferrostatin-1 μl 0.01% DMSO에 식염수 |
제형 | 1 pmol 의 Ferrostatin-1,콜라겐아제 주사 |
응용 분야 | Ferrostatin-1은 철사병의 특이 억제제로 혈구소가 유발하는 신경세포 사망과 철 퇴적을 예방하기 위해 기관형 해마 슬라이스 문화(OHSCs)에 사용되었습니다. 뇌출혈(ICH) 후 Ferrostatin-1을 치료한 쥐는 두뇌 보호와 신경 기능 개선이 현저했습니다. 게다가Ferrostatin-1은 지질 활성산소종의 생성을 줄이고 PTGS2 및 그 유전자제품 cyclooxygenase-2의 표현 수준을 완화시켰고 이러한 효과는 실험실 외와 실험실 내에서 모두 관찰되었습니다. |
References: | |
| Cas No. | 347174-05-4 | SDF | |
| Synonyms | Fer-1 | ||
| Canonical SMILES | NC1=C(NC2CCCCC2)C=CC(C(OCC)=O)=C1 | ||
| Formula | C15H22N2O2 | M.Wt | 262.35 |
| Solubility | 125mg/ml in DMSO | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.8117 mL | 19.0585 mL | 38.117 mL |
| 5 mM | 762.3 μL | 3.8117 mL | 7.6234 mL |
| 10 mM | 381.2 μL | 1.9059 mL | 3.8117 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
-
Related Biological Data
Interestingly, ferrostatin-1, an inhibitor of ferropto-sis in mammalian cells, significantly inhibited the antibacterialactivity of Fe(II)Snaq.
Ferrostatin-1 andLiproxstatin-1 were purchased from Glpbio (USA).
Nano Today 35 (2020): 100981. IF: 18.9615 -
Related Biological Data
Ferroptosis is triggered following FGFR4 inhibition in anti-HER2 resistant breast cancer cells. h The ratio of oxidized to nonoxidized lipids was assessed by flow cytometry following C11-BODIPY probe staining in rSKBR3 cells.
Cell death caused by roblitinib was partly rescued by cotreatment with the specific ferroptosis inhibitors ferrostatin-1 (1 μM)(GLPbio), liproxstatin-1 (500 nM) or the iron chelator deferoxamine (DFO, 100 μM).
Nature Communications 13.1 (2022): 2672. PMID: 36642338 IF: 16.6009 -
Related Biological Data
Cytotoxicity of HP NPs + RT after incubation with different ferroptosis inhibitors Fer1, VE, and GSH.
Cells were co-administered by HP NPs (hemin dose: 15 μg ·mL −1 ) with 50 nM Ferrostatin-1(GLPbio), 100 μM vitamin E, and 1 mM glutathione, and treated with radiotherapy (8 Gy).
Acta Biomaterialia (2023). PMID: 36642338 IF: 10.6335 -
Related Biological Data
Cell death pathway and cell morphology (n = 3). (a) The cell viability treated with LDG plus inhibitors of different cell death pathways and action mechanism.
To study the specific cell death pathway induced by liposomes,LDG was administrated in combination with Ferrostatin-1 (Fer-1, ferroptosis inhibitor, 16 μM)（GLPbio）, Desferrioxamine (100 μM), Z-VADFMK (50 μM), 3-MA (25μM), Necrostatin-1 (20 μM), and Nacetylcysteine (20 μM) to measure the cell viability rescuing profile using the CCK8 assay.
Nano Research, 2024: 1-17. IF: 9.8996 -
Related Biological Data
Suggesting that inhibition of LPO ameliorated GLY-triggered ferroptosis in TM3 cells. Consequently, the downregulation of GLY-induced cell viability.
To elucidate the involvement of ferroptosis in GLY-induced cytotoxicity, cells were pre-treated with 10 μM Fer-1（GLPbio） for 6 h and subsequently treated with 1mM GLY for 24 h to assess relevant indicators.
Science of The Total Environment (2024): 169927. PMID: 38199345 IF: 9.8003 -
Related Biological Data
Deoxycholic acid enhanced lipopolysaccharide-induced ferroptosis in intestinal epithelial cells (J) The content of GSH in each group of IEC-6 cells (n ¼ 5 in each group). (K) The content of MDA in each group of IEC-6 cells (n 1/4 5 in each group).
Glucose uptake ability of VSMCs was evaluated by using the fluores-cent glucose Ferrostatin-1 (Fer-1) (GlpBio, USA) according to the manufactur-er’s instruction. cells were plated onto coverslips and incubated with DMEM containing 10 μM Ferrostatin-1 (Fer-1) at room temperature for 1 h.Cell Death Dis.
Molecular Metabolism (2024): 101944. PMID: 38642891 IF: 8.1 -
Related Biological Data
Cells were incubated with different concentrations (0.25 and 0.5 mmol/L) of PA in the presence or absence of either 4 μmol/L Fer-1.
Cells were incubated with different concentrations (0, 0.125, 0.25, 0.5, and 1.0 mmol/L) of PA for 72 h. Intracellular iron levels were determined with an iron colorimetric assay kit, and lipid peroxidation was determined by the fluorescent probe C11 BODIPY 581/591 using flow cytometry.
Free Radical Bio Med(2021). -
Related Biological Data
A7r5 cells were treated with 100% cigarette smoke extract (CSE) for 4 h in the presence or absence of Fer-1 (5 μM), cell viability was assessed using the CCK8 assay (n = 3). (D-J) Changes in mRNA expression of hub genes after 4 h of 100%CSE treatment in the presence or absence of Fer-1 (5 μM) by qRT-PCR (n = 3).
Ferrostatin-1 (Fer-1, GC10380) was purchased from Glpbio Technology Inc. (Montclair, CA, USA) and dissolved in dimethyl sulfoxide (DMSO). In cell culture experiments, Fer-1 was treated 1 h before CSE treatment at a concentration of 5 μM.
Bioengineered Just-Accepted (2021).
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *