(-)-FINO2
|
| Catalog No.GC45248 |
(-)-FINO2는 선택적으로 철사망을 유도할 수 있는 유기 과산화물이고 철사망과 항암 치료의 기전을 연구하기 위해 사용된다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 869298-31-7
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
(-)-FINO2는 선택적으로 철사망을 유도할 수 있는 유기 과산화물이고 철사망과 항암 치료의 기전을 연구하기 위해 사용된다. (-)-FINO2는 글루타치온을 소진하고 GPX4 활성을 억제해서 철분에 의존하는 지질 과산화를 특이적으로 유도해서 철사망을 유발할 수 있다[2]. (-)-FINO2는 종양 억제, 신경퇴행성 질환, 허혈-재관류 손상과 같은 병리학적 과정에서 강균증의 역할을 조사하기 위해 사용할 수 있다[3-4].
체외 실험에서 (-)-FINO2 (20μM)로 인간 관절 연골세포 (C-28/I2, T/C-28/A2 세포 라인과 원발성 연골 세포)를 24시간 동안 처리하면 불안정한 Fe²⁺을 산화시키고 GPX4 활성을 간접적으로 억제해서 철사망을 유발해서 세포 생존율을 현저하게 감소시킨다[5]. HT-1080 섬유육종 세포를 (-)-FINO2 (10μM)로 24시간 동안 처리하면 철분에 의존하는 지질 과산화 경로를 통해 세포 사멸을 현저하게 유도한다[6].
References:
[1] Abrams RP, Carroll WL, Woerpel KA. Five-Membered Ring Peroxide Selectively Initiates Ferroptosis in Cancer Cells. ACS Chem Biol. 2016 May 20;11(5):1305-12.
[2] Lei Y, Peng X, Hu Y, et al. The Calcilytic Drug Calhex-231 Ameliorates Vascular Hyporesponsiveness in Traumatic Hemorrhagic Shock by Inhibiting Oxidative Stress and miR-208a-Mediated Mitochondrial Fission. Oxid Med Cell Longev. 2020 Dec 3;2020:4132785.
[3] JHuang Y, Ru Q, Ruan H, et al. Changyanning tablet alleviates Crohn's disease by inhibiting GPX4-mediated ferroptosis. J Ethnopharmacol. 2025 Feb 27;342:119415.
[4] Van Kessel ATM, Cosa G. Lipid-derived electrophiles inhibit the function of membrane channels during ferroptosis. Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2317616121.
[5] Wipplinger A, Bekric D, Ablinger C, et al. Cannabidiol Is a Potential Inhibitor of Ferroptosis in Human Articular Chondrocytes. J Cell Mol Med. 2025 Jul;29(13):e70592.
[6] Gaschler MM, Andia AA, Liu H, et al. FINO2 initiates ferroptosis through GPX4 inactivation and iron oxidation. Nat Chem Biol. 2018 May;14(5):507-515.
| 세포 실험 [1]: | |
세포 라인 | HT-1080섬유육종 세포 (철사망에 민감한 세포 라인) |
제조 방법 | HT-1080 세포는 37°C, 5% CO₂에서 10% 태아 소 혈청 (FBS)을 첨가한 DMEM에서 배양하였습니다. 세포는 10μM (-)-FINO2로 6시간 또는 24시간 동안 처리하였습니다. |
반응 조건 | 10μM; 6-24 시간 동안 |
응용 분야 | (-)-FINO2 (10 μM)은 철분에 의존하는 지질 과산화 경로를 통해 세포 사멸을 현저하게 유도합니다. |
References: | |
| Cas No. | 869298-31-7 | SDF | |
| Canonical SMILES | CC(C)(C)[C@@H](CC1)CC[C@]21CC(CCO)(C)OO2 | ||
| Formula | C15H28O3 | M.Wt | 256.4 |
| Solubility | 30 mg/ml in DMSO, 30 mg/ml DMF, 30 mg/ml in Ethanol | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.9002 mL | 19.5008 mL | 39.0016 mL |
| 5 mM | 780 μL | 3.9002 mL | 7.8003 mL |
| 10 mM | 390 μL | 1.9501 mL | 3.9002 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 38 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *