β-Glycerophosphate (sodium salt hydrate) (Synonyms: Glycerol 2-Phosphate) |
Catalog No.GC11806 |
β-글리세로인산 (나트륨 염 수화물)은 인산효소 억제제입니다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 13408-09-8
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
β-글리세로인산 (나트륨 염 수화물)은 강력한 단백질 인산화효소 억제제입니다 [1].
단백질 인산화효소는 대상 단백질의 인산화된 아미노산 잔기로부터 인산기를 제거하는 효소입니다. 단백질 인산화는 가장 일반적인 단백질 후번역 수정 중 하나입니다.
β-글리세로인산은 뼈 세포 광물화 연구에서 인 그룹 기부체 역할을 하며 강력한 단백질 인산화 효소 억제제입니다 [1][3]. MC3T3-E1, ROS 17/2.8 및 병아리 골모양 세포에서 β-글리세로인산은 무산소 글라이콜리시스 속도에 영향을 주지 않았지만 매질 Pi 수준을 증가시켜 뼈 세포에 의해 가수분해되었다는 것을 시사합니다. 매질 Pi 농도의 지역적 증가는 신속한 광물 침전을 유발합니다 [1]. BMP-2는 인간 골수 줄기 세포 (HBMSC) 및 인간 골모양 세포 (HOB)에서 베타-글리세로인산과 아스코르비크 애씨드의 존재하에서 매트릭스의 광물화를 유도합니다 [3]. 10mM의 β-글리세로인산은 염기성 포스파타아제 관련 메카니즘을 통해 배양된 혈관 평활근 세포의 칙화를 가속화시킵니다 [4].
References:
[1]. Chung CH, Golub EE, Forbes E, et al. Mechanism of action of beta-glycerophosphate on bone cell mineralization. Calcif Tissue Int. 1992 Oct;51(4):305-11.
[2]. Fujimoto H, Mabuchi I. Isolation of cleavage furrows from eggs of regular sea urchins and identification of furrow-specific proteins. J Biochem. 1997 Sep;122(3):518-24.
[3]. Lecanda F, Avioli LV, Cheng SL. Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2. J Cell Biochem. 1997 Dec 1;67(3):386-96.
[4]. Shioi A, Nishizawa Y, Jono S, et al. Beta-glycerophosphate accelerates calcification in cultured bovine vascular smooth muscle cells. Arterioscler Thromb Vasc Biol. 1995 Nov;15(11):2003-9.
Cas No. | 13408-09-8 | SDF | |
Synonyms | Glycerol 2-Phosphate | ||
Chemical Name | 2-(dihydrogen phosphate)-1,2,3-propanetriol, disodium salt, pentahydrate | ||
Canonical SMILES | [O-]P(OC(CO)CO)([O-])=O.[Na+].[Na+].O.O.O.O.O | ||
Formula | C3H7O6P • 2Na [5H2O] | M.Wt | 306.1 |
Solubility | ≥ 30.7mg/mL in Water, ≥ 14.3mg/mL in insoluble in DMSO | Storage | 4°C, protect from light |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 3.2669 mL | 16.3345 mL | 32.6691 mL |
5 mM | 0.6534 mL | 3.2669 mL | 6.5338 mL |
10 mM | 0.3267 mL | 1.6335 mL | 3.2669 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
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