(-)-p-Bromotetramisole Oxalate (Synonyms: (-)-p-Bromotetramisole, L-para-Bromotetramisole) |
| Catalog No.GC14520 |
(-)-p-Bromotetramisole Oxalate (L-p-bromotetramisole)은 강력하고 비특이적인 알칼리성 인산아세트아제 억제제이다.
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Cas No.: 62284-79-1
Sample solution is provided at 25 µL, 10mM.
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(-)-p-Bromotetramisole Oxalate (L-p-bromotetramisole)은 강력하고 비특이적인 알칼리성 인산아세트아제 억제제이다.
(-)-p-Bromotetramisole Oxalate (1 mM)은 cAMP 자극된 이오딘 유출을 증강시키고 그 자체로 cAMP 자극제보다 더 큰 유출을 자극함으로써 CHO 세포에서 인간 섬유증 유전체 조절기 (CFTR)의 개방을 촉진할 수 있을 수 있다[3]. PC12 세포에서 (-)-p-Bromotetramisole Oxalate (0.3 mM)은 이오노미신 자극된 노르에피네프린 (NA) 유출을 증강시킨다[4].
(-)-p-Bromotetramisole Oxalate (30 mg/kg; 정맥 주입)은 노르에피네프린으로 인한 평균 동맥혈압 (MABP) 상승과 신장혈관 저항을 현저하게 억제했다. 노르에피네프린에 대한 신장혈관 및 혈압 반응을 줄였다[5]. 체계적 주입 (-)-p-Bromotetramisole Oxalate (0.8 ml/min의 10 mM I-p-Bromotetramisole Oxalate; 정맥 주입)은 Sprague-Dawley 쥐에서 인산염의 분율 배설 (FEPi)을 증가시켰다[6].
References:[1]. Borgers M. The cytochemical application of new potent inhibitors of alkaline phosphatases. J Histochem Cytochem. 1973 Sep;21(9):812-24. doi: 10.1177/21.9.812. PMID: 4741290.[2]. Borgers M, Thoné F. The inhibition of alkaline phosphatase by L-p-bromotetramisole. Histochemistry, 1975, 44(3): 277-280.[3]. Lansdell KA, Kidd JF, et,al. Regulation of murine cystic fibrosis transmembrane conductance regulator Cl- channels expressed in Chinese hamster ovary cells. J Physiol. 1998 Nov 1;512 ( Pt 3)(Pt 3):751-64. doi: 10.1111/j.1469-7793.1998.751bd.x. PMID: 9769419; PMCID: PMC2231228.[4]. Kitamura T, Murayama T, et,al. Enhancement of Ca2+-induced noradrenaline release by vanadate in PC12 cells: possible involvement of tyrosine phosphorylation. Brain Res. 2000 Jan 31;854(1-2):165-71. doi: 10.1016/s0006-8993(99)02299-4. PMID: 10784118.[5]. Jackson EK, Zhang Y,et,al. Alkaline Phosphatase Inhibitors Attenuate Renovascular Responses to Norepinephrine. Hypertension. 2017 Mar;69(3):484-493. doi: 10.1161/HYPERTENSIONAHA.116.08623. Epub 2017 Jan 30. PMID: 28137984; PMCID: PMC5310812.[6]. Onsgard-Meyer M, McCoy AL, et,al. Effect of bromotetramisole on renal phosphate excretion. Proc Soc Exp Biol Med. 1996 Nov;213(2):193-5. doi: 10.3181/00379727-213-44050. PMID: 8931664.
| 세포 실험 [1]: | |
세포 라인 | PC12세포 |
제조 방법 | 미리 표시된 PC12 세포는 2 mM CaCl2를 포함한 조건에서 운반제나 5 µM 이오노미신과 함께 배양되었습니다. (-)-p-Bromotetramisole Oxalate (BTO)은 추가로 시험 혼합물에 추가되었습니다. |
반응 조건 | 0.3 mM |
응용 분야 | (-)-p-Bromotetramisole Oxalate (0.3 mM)은 이오노미신 자극된 노르에피네프린 (NA) 유출을 증강시킵니다. |
| 동물 실험 [2]: | |
동물 모형 | Sprague-Dawley 쥐 |
제조 방법 | 갑상선 절제된 쥐에서 0.8 ml/min의 10 mM (-)-p-Bromotetramisole Oxalate 체계적 주입 전후에 인산염의 분율 배설 (FEPi)을 측정했습니다. |
제형 | 0.8 ml/분의 10 mM (-)-p-Bromotetramisole Oxalate; ivgtt |
응용 분야 | 체계적 주입 (-)-p-Bromotetramisole Oxalate은 쥐에서 인산염의 분율 배설 (FEPi)을 증가시킵니다. |
참고문헌: [1]. Kitamura T, Murayama T, et,al. Enhancement of Ca2+-induced noradrenaline release by vanadate in PC12 cells: possible involvement of tyrosine phosphorylation. Brain Res. 2000 Jan 31;854(1-2):165-71. doi: 10.1016/s0006-8993(99)02299-4. PMID: 10784118.[2]. Onsgard-Meyer M, McCoy AL, et,al. Effect of bromotetramisole on renal phosphate excretion. Proc Soc Exp Biol Med. 1996 Nov;213(2):193-5. doi: 10.3181/00379727-213-44050. PMID: 8931664. | |
| Cas No. | 62284-79-1 | SDF | |
| Synonyms | (-)-p-Bromotetramisole, L-para-Bromotetramisole | ||
| Canonical SMILES | BrC(C=C1)=CC=C1[C@H]2N=C3SCCN3C2.OC(C(O)=O)=O | ||
| Formula | C13H13BrN2O4S | M.Wt | 373.22 |
| Solubility | ≥ 18.65mg/mL in DMSO | Storage | Desiccate at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.6794 mL | 13.3969 mL | 26.7938 mL |
| 5 mM | 0.5359 mL | 2.6794 mL | 5.3588 mL |
| 10 mM | 0.2679 mL | 1.3397 mL | 2.6794 mL |
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Quality Control & SDS
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- Purity: >99.00%
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Average Rating: 5 (Based on Reviews and 1 reference(s) in Google Scholar.)
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