Protease Inhibitor Cocktail (EDTA-Free,100× in DMSO) |
| Catalog No.GK10014 |
세포 라이제이트나 조직 추출물에서 단백질 안정성을 증가시키기 위해 프로테아제 억제제 칵테일이 사용됩니다.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability.
Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein production is greatly halted and degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of inhibitors of those enzymes known to be present.
Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability. The cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates.
Protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. This cocktail is supplied as a ready-to-use solution in DMSO.
| Ingredient | Summary | CAS Number | Targets |
| Aprotinin | Inhibitor of bovine pancreatic trypsin | 9087-70-1 | Proteases|Serine Protease |
| Bestatin | Aminopeptidase inhibitor | 58970-76-6 | Proteases|Aminopeptidase |
| E-64 | Cysteine protease inhibitor,irriversible | 66701-25-5 | Proteases|Cathepsin |
| Leupeptin, Microbial | Inhibitor of serine and cysteine proteases | 103476-89-7 | Proteases|Serine Protease |
| Pepstatin A | Aspartic proteinases inhibitor | 26305-03-3 | Proteases|Other Proteases |
| AEBSF.HCl | Serine protease inhibitor | 30827-99-7 | Proteases|Serine Protease |
Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts), before assaying.
Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
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Average Rating: 5 (Based on Reviews and 24 reference(s) in Google Scholar.)
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