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Pyocyanin (Synonyms: Sanasin,Sanazin,Pyocyanine)

Catalog No.GC13137

피오시아닌은 생물학적으로 활성화된 페나진 색소입니다.

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Pyocyanin Chemical Structure

Cas No.: 85-66-5

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50mg
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Sample solution is provided at 25 µL, 10mM.

Product has been cited by 1 publications

Product Documents

Quality Control & SDS

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Protocol

Cell experiment [1]:

Cell lines

Human embryonic lung epithelial cell line, L-132

Preparation Method

Cell cultures in 96 well plates were developed by adding 0.2 ml cell suspension in growth medium containing approximately 5 × 105 cells ml−1 and incubating for 12 h at appropriate temperature.

Reaction Conditions

Pyocyanin was prepared with different concentrations in growth medium. Then added to the wells to attain final strength ranging from 6.25 to 200 µg ml−1 for XTT (mitochondrial activity), neutral red up take (plasma membrane damage) and SRB (protein synthesis), 0–200 µg ml−1 for LDH and H2O2, and 25–200 µg ml−1 for glucose consumption in triplicate for each concentration. Incubate for 24 h.

Applications

The cytotoxicity of pyocyanin could be assessed by this experiment, facilitating pyocyanin’s safe usage toxicity. Higher concentrations of pyocyanin (175 and 200 mg l−1) only caused significant morphological changes such as clumping, and necrosis as visualized microscopically in L-132 cell line. Human cell membrane was found to be susceptible to oxidative damage induced by pyocyanin.

Animal experiment [2]:

Animal models

Male C57BL/6J mice (8-10 weeks, 20-30 g)

Preparation Method

Mice were housed under controlled laboratory conditions, maintained on a 12 h day and night cycle. Then mice were acclimatized for 5 days to the lab conditions and handling prior to the actual beginning of the experiments. Mice were administered 0.9% saline as control, and pyocyanin by intranasal route. Another group was treated with LPS (P.aeruginosa) by intraperitoneal route to mimic the effects of ongoing infection on tissue permeability, and pyocyanin was instilled by intranasal route 3h after LPS injection.

Dosage form

Pyocyanin (50 µg/50 µL in 0.9% saline); 0.9% saline (50 µL); LPS (3 mg/kg)

Applications

Pyocyanin could be diffused into systemic circulation, which was not influenced by the pre-exposure to pseudomonal infestation. This experiment detected the plasma concentration of intranasally administered Pyocyanin. Furthermore, localized administration of Pyocyanin was able to elicit changes to behavior and a systemic pro-inflammatory and pro-oxidant effect.

References:

[1]. Priyaja P, et al. Pyocyanin induced in vitro oxidative damage and its toxicity level in human, fish and insect cell lines for its selective biological applications. Cytotechnology. 2016 Jan;68(1):143-155.

[2]. Arora D, et al. Pyocyanin induces systemic oxidative stress, inflammation and behavioral changes in vivo. Toxicol Mech Methods. 2018 Jul;28(6):410-414.

Background

피오시아닌은 Pseudomonas aeruginosa 균에 의해 생산되는 생물학적으로 활성화된 페나진 색소로, 다양한 약리학적 조성에서 질산염(NO) 상대제로 작용하며 바이오센서의 매개체 역할을 합니다. 또한, Pyocyanin은 미생물 연료 전지에서 박테리아 전자 이동을 가능케하는 전자 셔틀로 사용될 수 있습니다. 게다가, Pyocyanin은 넓은 스펙트럼의 항생활성을 가지고 있으며 어쿠어컬처 시스템에서 병원성 바이브리오 성장 억제를 위해 Pseudomonas가 생성하는 주요 분자로 확인되었습니다.[1]

체외 연구에서 Pyocyanin의 세포독성을 측정하였다. 결과는 L-132 세포가 Pyocyanin 유도 독성에 민감함을 나타내었다. 미토콘드리아 탈수소효소 활동 억제에 대한 Pyocyanin의 IC50 값은 112.01 ± 23.73 mg l−1이었다. 플라즈마 막 손상 유발에 대한 Pyocyanin의 IC50 값은 21.79 ± 14.23 mg l−1이었다. 게다가, 단백질 합성 억제에 대한 Pyocyanin의 IC50 값은 32.57 ± 16.52 mg l−1이었다. Pyocyanin 농도가 25 mg l-1일 때, L-132 세포에서는 미토콘드리아 활동 저해율이 3.9 %, 플라즈마 막 손상률이 47.3 % 및 단백질 합성 억제율이 26 .6%로 관찰되었다. 그러나 낮은 농도 (6 .25mg /l)에서는 독성이 없으며, 고농도 (200mg/l)에서는 각각 64.8 %, 72.8 % 및 91.7 %의 값을 보였다.[1]

체내 연구에서 Pyocyanin이 인간 호흡기 점막의 섬유모 박동을 늦추는 능력이 있음을 입증하였다. 방사성 동맥혈구로 표지된 기관지 점액 속도에 대한 Pyocyanin의 영향은 마취된 기니피그에서 시험되었다. Pyocyanin의 효과는 시작이 더디며, 3시간 후 600ng으로 주입하여 기관지 점액 속도를 60% 감소시켰으며 회복하지 않았다. 반면, Pyocyanin과 1-하이드록시페나진 조합은 단독으로 투여한 동일 용량의 1-하이드록시페나진과 같은 초기 신속한 감속을 유발하지만, 이어서 나타난 Pyocyanin에 의해 인한 감속은 단독으로 투여할 때보다 크게 나타났다. 이 연구는 P.aeruginosa의 제품인 Pyocyanin 등이 호흡기 점막 내 침습을 촉진하는 한 가지 메커니즘을 보여준다.

References:
[1]. Priyaja P, et al. Pyocyanin induced in vitro oxidative damage and its toxicity level in human, fish and insect cell lines for its selective biological applications. Cytotechnology. 2016 Jan;68(1):143-155.
[2]. Arora D, et al. Pyocyanin induces systemic oxidative stress, inflammation and behavioral changes in vivo. Toxicol Mech Methods. 2018 Jul;28(6):410-414.

Chemical Properties

Cas No. 85-66-5 SDF
Synonyms Sanasin,Sanazin,Pyocyanine
Chemical Name 5-methyl-1(5H)-phenazinone
Canonical SMILES Cn1c2ccccc2nc2c(=O)cccc12
Formula C13H10N2O M.Wt 210.2
Solubility 5mg/ml in ethanol, or in DMSO; 2.5mg/ml in DMF Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 4.7574 mL 23.7869 mL 47.5737 mL
5 mM 0.9515 mL 4.7574 mL 9.5147 mL
10 mM 0.4757 mL 2.3787 mL 4.7574 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 18 reference(s) in Google Scholar.)

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