SenTraGor™ Cell Senescence Reagent (Synonyms: GL13, SBB-A-B, SBB-Analogue (GL13) Biotin) |
| Catalog No.GC49723 |
A cellular senescence detection reagent
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2171036-89-6
Sample solution is provided at 25 µL, 10mM.
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SenTraGor™ cell senescence reagent is a detection reagent for lipofuscin, a nondegradable lysosomal marker of cellular senescence.1 It is composed of a Sudan Black B (SBB) derivative conjugated to biotin and can be detected using an anti-biotin antibody. SenTraGor™ cell senescence reagent has been used to detect lipofuscin accumulation in Vero E6 cells infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and primary non-small cell lung cancer (NSCLC) samples as well as soluble lipofuscin in serum from patients with various diseases, including dementia and rheumatoid arthritis.2,3,4
1.Evangelou, K., Lougiakis, N., Rizou, S.V., et al.Robust, universal biomarker assay to detect senescent cells in biological specimensAging Cell16(1)192-197(2017)
2.Evangelou, K., Veroutis, D., Paschalaki, K., et al.Pulmonary infection by SARS-CoV-2 induces senescence accompanied by an inflammatory phenotype in severe COVID-19: Possible implications for viral mutagenesisEur. Respir. J.2102951(2022)
3.Giatromanolaki, A., Kouroupi, M., Balaska, K., et al.A novel lipofuscin-detecting marker of senescence relates with hypoxia, dysregulated autophagy and with poor prognosis in non-small-cell-lung cancerIn Vivo34(6)3187-3193(2020)
4.Rizou, S.V., Evangelou, K., Myrianthopoulos, V., et al.A Novel Quantitative Method for the Detection of Lipofuscin, the Main By-Product of Cellular Senescence, in FluidsCellular senescence: Methods and protocols. Methods in molecular biology 1896119-138(2019)
SenTraGor reagent for cell senescent detection is supplied as a solid powder. A working solution can bemade by dissolving the SenTraGor reagent in 100% (absolute) ethanol, following the guidelines below.
Dilution:
Add 350-375 μL (2 mg SenTraGor),875-935 μL (5 mg SenTraGor), 1.75-1.875 mL(10 mg SenTraGor)or 3.5-3.75 mL (20 mg SenTraGor) 100% ethanol to the vial with the reagent and cover it with its cap and Parafilm.
Incubate at 56℃ in a water bath for 120 minutes until the reagent is completely dissolved. Store at room temperature.
Caution:
During the entire process the dye container must be sealed air tight to prevent evaporation of ethanol.
Ethanol evaporation would lead to precipitation of the saturated dye solution in tissues.
If a non-specific (high background staining, which results in a poor signal-to-noise ratio) reaction of thereagent is observed, adjust the final volume to 375 μL (2 mg SenTraGor), 935 μL (5 mg SenTraGor), 1.875 mL(10 mg SenTraGor), or 3.75 mL (20 mg SenTraGor).
| Cas No. | 2171036-89-6 | SDF | |
| Synonyms | GL13, SBB-A-B, SBB-Analogue (GL13) Biotin | ||
| Canonical SMILES | O=C(N1)N[C@](CS2)([H])[C@@]1([H])[C@@H]2CCCCC(OCC(C)(N3)NC4=CC=C(C5=C4C3=CC=C5)/N=N/C6=CC=C(/N=N/C7=CC=CC=C7)C8=C6C=CC=C8)=O | ||
| Formula | C39H38N8O3S | M.Wt | 698.8 |
| Solubility | DMF: 2 mg/mL,DMSO: 2 mg/mL,Ethanol: Slightly soluble,PBS (pH 7.2): 0.3 mg/mL | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.431 mL | 7.1551 mL | 14.3102 mL |
| 5 mM | 286.2 μL | 1.431 mL | 2.862 mL |
| 10 mM | 143.1 μL | 715.5 μL | 1.431 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 2 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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