Sephadex LH 20 |
| Catalog No.GC61779 |
Sephadex LH 20은 지질, 스테로이드, 지방산, 호르몬, 비타민 등과 유기 용매 이동상을 함께 사용해서 겔 투과, 정상상 분리 및 흡착 크로마토그래피에 사용되는 교차 결합된 덱스트란 기반 수지이다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 9041-37-6
Sample solution is provided at 25 µL, 10mM.
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Sephadex LH 20은 지질, 스테로이드, 지방산, 호르몬, 비타민 등과 유기 용매 이동상을 함께 사용해서 겔 투과, 정상상 분리 및 흡착 크로마토그래피에 사용되는 교차 결합된 덱스트란 기반 수지이다. Sephadex LH 20은 Sephadex G-25의 하이드록시프로필 유도체이다. 그의겔 매트릭스는 극성 유기 용매나 수성-유기 혼합 시스템에서의 호환성을 현저하게 향상시키기 위해 수정되었다[3]. Sephadex LH 20 충전재는 스테로이드, 테르펜류, 지질, 저분자량 펩타이드와 같은 천연물질의 제조와 정제에 일반적으로 사용된다[4, 5].
References:
[1] Cansu A G. Investigating the adsorption behavior of simple benzene derivatives on cross-linked dextran polymer[J]. 2023.
[2] Kale A, Zhu F, Cheryan M. Separation of high-value products from ethanol extracts of corn by chromatography[J]. Industrial Crops and Products, 2007, 26(1): 44-53.
[3] Tian Y, Liimatainen J, Puganen A, et al. Sephadex LH-20 fractionation and bioactivities of phenolic compounds from extracts of Finnish berry plants[J]. Food research international, 2018, 113: 115-130.
[4] Mottaghipisheh J, Iriti M. Sephadex® LH-20, isolation, and purification of flavonoids from plant species: A comprehensive review[J]. Molecules, 2020, 25(18): 4146.
[5] Dinan L, Harmatha J, Lafont R. Chromatographic procedures for the isolation of plant steroids[J]. Journal of chromatography A, 2001, 935(1-2): 105-123.
| Cas No. | 9041-37-6 | SDF | |
| Canonical SMILES | [Sephadex LH 20] | ||
| Formula | C11H9N3NaO2 | M.Wt | 238.20 |
| Solubility | H2O : < 0.1 mg/mL (insoluble) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 4.1982 mL | 20.9908 mL | 41.9815 mL |
| 5 mM | 839.6 μL | 4.1982 mL | 8.3963 mL |
| 10 mM | 419.8 μL | 2.0991 mL | 4.1982 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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Average Rating: 5 (Based on Reviews and 27 reference(s) in Google Scholar.)
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