T7 Tag Peptide |
| 카탈로그 번호: GP10156 |
T7 태그 펩티드는 주요 T7 캡시드 단백질인 gp 10의 N-말단 11개 잔기에서 파생된 단백질 태그입니다.
Sample solution is provided at 25 µL, 10mM.
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The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The T7 tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems [1].
References:
1. Studier, F.W. and Moffatt, B.A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189, 113-30.
| Cas No. | 245445-88-9 | SDF | |
| 동의어 | H-Met-Ala-Ser-Met-Thr-Gly-Gly-Gln-Gln-Met-Gly-OH | ||
| Chemical Name | T7 Tag | ||
| Canonical SMILES | CC(C(C(=O)NCC(=O)NCC(=O)NC(CCC(=O)N)C(=O)NC(CCC(=O)N)C(=O)NC(CCSC)C(=O)NCC(=O)O)NC(=O)C(CCSC)NC(=O)C(CO)NC(=O)C(C)NC(=O)C(CCSC)N)O | ||
| Formula | C41H71N13O16S3 | M.Wt | 1098.27 |
| 용해도 | >54.9mg/mL in DMSO | Storage | Desiccate at -20°C |
| 일반적인 팁 | 높은 용해도를 얻기 위해 튜브를 37℃에서 데운 후 초음파 욕조에서 잠시 흔들어 주십시오. 원액은 -20℃ 이하에서 수개월간 보관할 수 있습니다. | ||
| 배송 조건 | 평가 샘플 솔루션: 블루아이스와 함께 배송합니다. 다른 모든 가능한 크기: 요청 시 RT 또는 블루아이스와 함께 배송합니다. | ||
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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