>>Signaling Pathways>> GPCR/G protein>> S1P receptor inhibitor>>W123

W123

Catalog No.GC17720

S1P1 antagonist

Products are for research use only. Not for human use. We do not sell to patients.

W123 Chemical Structure

Cas No.: 1345982-24-2

Size 가격 재고 수량
1mg
US$121.00
재고 있음
5mg
US$503.00
재고 있음
10mg
US$888.00
재고 있음

Tel:(909) 407-4943 Email: sales@glpbio.com

고객 리뷰

Based on customer reviews.

  • GlpBio Citations

    GlpBio Citations
  • Bioactive Compounds Premium Provider

    Bioactive Compounds Premium Provider

Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

View current batch:

Background

W123, an analog of FTY720, is a competitive antagonist of sphingosine-1-phosphate (S1P) type 1 receptor (S1P1), which is measured by GTPγS activation, cell migration, ligand-induced receptor internalization, and mitogen-activated protein kinase recruitment.

S1P plays a vital role in the entire human body, involving in mediating skin, vascular endothelial, and immune cells. S1P1, also known as EDG-1, is one of the five high affinity G protein-coupled S1P receptors and mediates lymphoid, endothelial, and neuronal cell responses to S1P.

In vitro: W123 reversed the effects of S1P1 agonists, FTY720, p-FTY720, and SEW2871. Superoxide dismutase and catalase activity level seen in W123-treated groups were reversely improved via antagonizing the S1P receptor induced by W123. W123 reduced phosphorylated levels of PI3K, Akt, and FoxO3a in different ways, indicating that the activation of S1P1 receptor in the context of oxidative stress elicited the phosphorylation/activation of PI3K/Akt and thus the phosphorylation/inactivation of FoxO3a which in turn promoted PC12 cell survival [1].

In vivo: Up to now, in vivo study of W123 is still in the development stage.

Reference:
[1].  Safarian, F., Khallaghi, B., Ahmadiani, A., & Dargahi, L. Activation of S1P1 Receptor Regulates PI3K/Akt/FoxO3a Pathway in Response to Oxidative Stress in PC12 Cells. Journal of Molecular Neuroscience. 2014; 56(1): 177-187.

Chemical Properties

Cas No. 1345982-24-2 SDF
Chemical Name 3-(2-(3-hexylphenylamino)-2-oxoethylamino)propanoic acid
Canonical SMILES OC(=O)CCNCC(=O)Nc1cccc(CC2CCCCC2)c1
Formula C17H26N2O3 M.Wt 306.4
Solubility 5mg/mL in DMSO or in DMF Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 3.2637 mL 16.3185 mL 32.6371 mL
5 mM 0.6527 mL 3.2637 mL 6.5274 mL
10 mM 0.3264 mL 1.6319 mL 3.2637 mL
  • Molarity Calculator

  • Dilution Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).

Calculate

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

리뷰

Review for W123

Average Rating: 5 ★★★★★ (Based on Reviews and 40 reference(s) in Google Scholar.)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%
Review for W123

GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.

Required fields are marked with *

You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.