YC 1 |
| Catalog No.GC16406 |
YC 1은 아르기노산산화아세틸산 사이클라이제（sGC）를 활성화시키는 노산산화아르기닌 종속적이지 않은 활성화제이고 낮은 산소 유도 인자-1α（HIF-1α）의 억제제이다.
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Cas No.: 170632-47-0
Sample solution is provided at 25 µL, 10mM.
YC 1은 아르기노산산화아세틸산 사이클라이제（sGC）를 활성화시키는 노산산화아르기닌 종속적이지 않은 활성화제이고 낮은 산소 유도 인자-1α（HIF-1α）의 억제제이다.
YC-1(0.01-100 µM; 24/48 시간)은 윔트 신호를 억제하고 간세포암(HCC) 세포의 증식을 억제한다[4]. 소라페닉과 YC-1(10 µmol/L; 48 시간)의 조합은 HepG2, BEL-7402, HCCLM3 세포의 증식과 군체 형성을 동시에 억제한다[5]. YC-1 세포 배양액에서의 VEGF 단백질 수준은 저산소 조건에서 성장한 미처리 세포의 매체에서 VEGF 단백질 수준에 비해 용액-의존적이다[6].
YC-1 처리(30 µg/g i.p; 2주)를 받은 쥐의 종양은 HIF-1α 수준이 낮고, 혈관화 정도가 적으며 HIF-1 유도 유전자의 수준도 통제조보다 낮은다[6]. 10 mg/kg YC-1(i.p; 이식 후 7일부터 매 3일)은 4T1와 MDA-MB-231 종양의 성장을 현저하게 억제한다[7]. 세보플루란 후처리가 저산소 뇌혈공급부전 손상 후 신경 보호를 제공할 수 있다. 저산소 유도 인자-1α 억제제 YC-1을 1.52 µg으로 왼쪽 측두실에 주입하여 저산소 뇌혈공급부전 손상 30분 전에 주사하면 쥐에서 세보플루란으로 인한 신경 보호를 되돌릴 수 있다[8].
References:
[1]. Martin E, Lee YC, et,al. YC-1 activation of human soluble guanylyl cyclase has both heme-dependent and heme-independent components. Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):12938-42. doi: 10.1073/pnas.231486198. Epub 2001 Oct 30. PMID: 11687640; PMCID: PMC60803.
[2]. Purohit R, Fritz BG, et,al. YC-1 binding to the β subunit of soluble guanylyl cyclase overcomes allosteric inhibition by the α subunit. Biochemistry. 2014 Jan 14;53(1):101-14. doi: 10.1021/bi4015133. Epub 2013 Dec 30. PMID: 24328155; PMCID: PMC3914721.
[3]. Nayak BK, Shanmugasundaram K, et,al. HIF-1 Mediates Renal Fibrosis in OVE26 Type 1 Diabetic Mice. Diabetes. 2016 May;65(5):1387-97. doi: 10.2337/db15-0519. Epub 2016 Feb 23. PMID: 26908870; PMCID: PMC4839204.
[4]. Wu JY, Shih YL, et,al. YC-1 Antagonizes Wnt/β-Catenin Signaling Through the EBP1 p42 Isoform in Hepatocellular Carcinoma. Cancers (Basel). 2019 May 13;11(5):661. doi: 10.3390/cancers11050661. PMID: 31086087; PMCID: PMC6562864.
[5]. Kong J, Kong F, et,al. YC-1 enhances the anti-tumor activity of sorafenib through inhibition of signal transducer and activator of transcription 3 (STAT3) in hepatocellular carcinoma. Mol Cancer. 2014 Jan 13;13:7. doi: 10.1186/1476-4598-13-7. PMID: 24418169; PMCID: PMC3895679.
[6]. Yeo EJ, Chun YS, et,al. YC-1: a potential anticancer drug targeting hypoxia-inducible factor 1. J Natl Cancer Inst. 2003 Apr 2;95(7):516-25. doi: 10.1093/jnci/95.7.516. PMID: 12671019.
[7]. Li Y, Zhang MZ, et,al. HIF-1α inhibitor YC-1 suppresses triple-negative breast cancer growth and angiogenesis by targeting PlGF/VEGFR1-induced macrophage polarization. Biomed Pharmacother. 2023 May;161:114423. doi: 10.1016/j.biopha.2023.114423. Epub 2023 Feb 21. PMID: 36822023.
[8]. Gao QS, Zhang YH, et,al. Brief inhalation of sevoflurane can reduce glial scar formation after hypoxic-ischemic brain injury in neonatal rats. Neural Regen Res. 2021 Jun;16(6):1052-1061. doi: 10.4103/1673-5374.300456. PMID: 33269750; PMCID: PMC8224129.
| 세포 실험 [1]: | |
세포 라인 | Hep3B 세포 |
제조 방법 | 세포는 플레이트에 올려져 YC-1이나 DMSO로 5분 동안 처리되어 정상 산소 및 저산소 조건에서 VEGF 수준을 측정했습니다. |
반응 조건 | 0.01-10µM YC-1；24시간 동안 |
응용 분야 | 저산소 조건에서 성장한 미처리 세포에 비해 YC-1 세포 배양액의 VEGF 단백질 수준이 용액-의존적으로 감소했습니다. |
| 동물 실험 [2]: | |
동물 모형 | 수컷 누드 (BALB/cAnNCrj-nu/nu) 쥐 |
제조 방법 | 암을 가진 쥐는 매일 내복 주사로 벤더블이나 YC-1(30 µg/g)을 2주 동안 투여받았습니다. |
제형 | 30 µg/g YC-1; i.p;2주 동안 |
응용 분야 | YC-1 처리를 받은 쥐의 종양은 HIF-1α 수준이 낮고 혈관화 정도가 적으며 HIF-1 유도 유전자의 수준이 통제조보다 낮습니다. |
참고문헌: [1]. Yeo EJ, Chun YS, et,al. YC-1: a potential anticancer drug targeting hypoxia-inducible factor 1. J Natl Cancer Inst. 2003 Apr 2;95(7):516-25. doi: 10.1093/jnci/95.7.516. PMID: 12671019. | |
| Cas No. | 170632-47-0 | SDF | |
| Chemical Name | (5-(1-benzyl-1H-indazol-3-yl)furan-2-yl)methanol | ||
| Canonical SMILES | OCC1=CC=C(C2=NN(CC3=CC=CC=C3)C4=CC=CC=C42)O1 | ||
| Formula | C19H16N2O2 | M.Wt | 304.34 |
| Solubility | ≥ 30.4mg/mL in DMSO | Storage | Store at RT |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.2858 mL | 16.429 mL | 32.858 mL |
| 5 mM | 0.6572 mL | 3.2858 mL | 6.5716 mL |
| 10 mM | 0.3286 mL | 1.6429 mL | 3.2858 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
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Quality Control & SDS
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- Purity: >99.00%
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Average Rating: 5 (Based on Reviews and 13 reference(s) in Google Scholar.)
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