L 012 sodium salt |
| Catalog No.GC13908 |
L 012 sodium salt is a luminol-based chemiluminescent probe.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 143556-24-5
Sample solution is provided at 25 µL, 10mM.
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L 012 sodium salt is a luminol-based chemiluminescent probe. Detects NAPDH oxidase-derived reactive oxygen and nitrogen species (ROS and RNS). Active in vitro and in vivo[1].
Reference:
[1].Daiber A, August M, Baldus S, et al. Measurement of NAD (P) H oxidase-derived superoxide with the luminol analogue L-012[J]. Free Radical Biology and Medicine, 2004, 36(1): 101-111.
This solution is for guidance only, please modify according to your actual needs.
1.Solution Preparation
Take appropriate amount of powder dissolved in pure water to make 20mM storage solution, the storage liquor is kept frozen to avoid repeated freezing and thawing. In the subsequent experiments, PBS (pH 7.5) can be used to dilute the working solution.
2. Cellular ROS imaging[1]
(1) Cell treatment: cells were grown to 90% confluence on a white transparent bottom 96-well culture plate and stimulated using different concentrations of H2O2.
(2) L 012 sodium salt incubation: the cell culture medium was replaced with a solution of PBS (pH 7.4) supplemented with L 012 sodium salt (400µM) for 5-10min.
(3) Fluorescence detection: fluorescence values were detected at 450nm using an enzyme marker.
2. In vivo ROS imaging[2]
(1) Preparation of working solution: take appropriate amount of mother liquor and dissolve it in PBS to make 30-60mg/kg.
(2) Animal treatment: anaesthetize the mice with isoflurane in advance, and dehair the mice at the imaging place.
(3) Dissolved L 012 sodium saltwas injected intraperitoneally into the body of mice at 250μL each.
(4) After the injection of L 012 sodium salt, the luminescence signal was detected using a bioluminescence imager, and images were acquired every two minutes for a total of 50min.
Reference:
[1] Al Ghouleh I, Meijles D N, Mutchler S, et al. Binding of EBP50 to Nox organizing subunit p47 phox is pivotal to cellular reactive species generation and altered vascular phenotype[J]. Proceedings of the National Academy of Sciences, 2016, 113(36): E5308-E5317.
[2] Rice, Christopher M., et al.Tumour-elicited neutrophils engage mitochondrial metabolism to circumvent nutrient limitations and maintain immune suppression. Nature communications 9.1 (2018): 5099.
| Cas No. | 143556-24-5 | SDF | |
| Chemical Name | sodium 8-amino-5-chloro-4-hydroxy-7-phenylpyrido[3,4-d]pyridazin-1-olate | ||
| Canonical SMILES | ClC1=NC(C2=CC=CC=C2)=C(N)C3=C1C(O)=NN=C3[O-].[Na+] | ||
| Formula | C13H8CIN4NaO2 | M.Wt | 310.67 |
| Solubility | <15.53mg/ml in Water; <31.07mg/ml in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.2188 mL | 16.0942 mL | 32.1885 mL |
| 5 mM | 0.6438 mL | 3.2188 mL | 6.4377 mL |
| 10 mM | 0.3219 mL | 1.6094 mL | 3.2188 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 11 reference(s) in Google Scholar.)
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