L-690330 |
| Catalog No.GC16451 |
competitive inhibitor of inositol monophosphatase (IMPase)
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 142523-38-4
Sample solution is provided at 25 µL, 10mM.
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L-690,330 is a competitive inhibitor of inositol monophosphatase (IMPase) with a Ki value of between 0.2 and 2 μM depending on the source of IMPase [1].
In the phosphatidylinositol (PI) cycle cell signalling pathway, IMPase is involved as a key enzyme. Inositol monophosphates, inositol(1)phosphate [Ins(l)P], inositol(3)phosphate [Ins(3)P], and inositol(4)phosphate [Ins(4)P] are hydrolyzed by IMPase and produce free inositol. Free inositol can then be used to synthesize PI [1].
In carbachol-stimulated m1 CHO cells, the treatment with L-690,330 alone did not increase the amount of [3H]InsP1. In the absence of L-690,330, the treatment with carbachol alone increased levels of [3H]InsP1, resulted in levels ranging from 20 to 100% above basal [3H]InsP1 levels. In the presence of carbachol, lithium at 10 mM increased the amount of [3H]InsP1 by approximate five folds (relative to [3H]InsP1 levels in carbachol-stimulated cells) and the EC50 value of this effect was about 1 mM. In carbachol-stimulated cells, L-690,330 at 10 mM resulted in only ~40% of the maximum response seen in the case with lithium treatment [1].
In mouse brains, in the absence of L-690,330, pilocarpine produced approximately doubled levels of Ins(1)P. In the absence of pilocarpine, L-690,330 did not affect Ins(1)P levels. Under pilocarpine-stimulated conditions, treatment with L-690,330 resulted in a pronounced accumulation of Ins(1)P, with the maximum level observed 1 h after injection. The maximum was of an approximate four-fold elevation [1].
Reference:
[1]. Atack JR, Cook SM, Watt AP, et al. In Vitro and In Vivo Inhibition of Inositol Monophosphatase by the Bisphosphonate L-690,330. Journal of neurochemistry, 1993, 60(2): 652-658.
| Cell experiment [1]: | |
|
Cell lines |
M1 CHO cells |
|
Preparation method |
The solubility of this compound in sterile water is 100 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months. |
|
Reacting condition |
0.1 ~ 10 mM |
|
Applications |
In Carbachol-stimulated m1 CHO cells, L-690,330 alone did not increase the amount of [3H]InsP1; lithium alone slightly elevated the amount of [3H]InsP1; Carbachol alone increased the amount of [3H]InsP1 ranging from 20 to 100%. |
| Animal experiment [1]: | |
|
Animal models |
Pilocarpine-stimulated mice |
|
Dosage form |
0 ~ 1 mmol/kg; s.c. |
|
Applications |
In Pilocarpine-stimulated mice, L-690,330 dose-dependently increased brain Ins(1)P levels, with the ED50 values of 0.3 mmol/kg. L-690,330 induced a maximal increase in the Ins(1)P level (about 4-fold) 1 hr after injection. |
|
Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
|
References: [1]. Atack JR, Cook SM, Watt AP, et al. In Vitro and In Vivo Inhibition of Inositol Monophosphatase by the Bisphosphonate L-690,330. Journal of neurochemistry, 1993, 60(2): 652-658. |
|
| Cas No. | 142523-38-4 | SDF | |
| Chemical Name | (1-(4-hydroxyphenoxy)ethane-1,1-diyl)diphosphonic acid | ||
| Canonical SMILES | OP(O)(C(OC(C=C1)=CC=C1O)(C)P(O)(O)=O)=O | ||
| Formula | C8H12O8P2 | M.Wt | 298.13 |
| Solubility | <29.81mg/ml in Water | Storage | 4°C, sealed storage, away from moisture |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.3542 mL | 16.7712 mL | 33.5424 mL |
| 5 mM | 0.6708 mL | 3.3542 mL | 6.7085 mL |
| 10 mM | 0.3354 mL | 1.6771 mL | 3.3542 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 39 reference(s) in Google Scholar.)
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