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L-NIO dihydrochloride

Catalog No.GC12867

inhibitor of nitric oxide (NO) synthase

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L-NIO dihydrochloride Chemical Structure

Cas No.: 159190-44-0

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10mg
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50mg
$529.00
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

IC50: 65 M

L-NIO dihydrochloride is an inhibitor of nitric oxide (NO) synthase (iNOS, eNOS and nNOS). An NO synthase is inducible by immunological stimuli such as endotoxin (LPS) and various cytokines, generating nitric oxide (NO) in phagocytic cells including neutrophils and macrophages. Generation of NO by macrophages has been revealed to kill tumour cells due to the inactivation of ironsulphur centres of mitochondrial enzymes.

In vitro: To protect against inflammatory injury stimulated by activated neutrophils, the ability of L-arginine (N-iminoethyl-L-ornithine (L-NIO) was studied in rats, following intradermal or intrapulmonary deposition of immune complexes [1]. The protective effect of L-NIO in the skin was reversed in a dose-dependent manner by the presence of L-arginine, rather than by D-arginine. The protective effects of L-NIO L-Arginine were reversed also in immune complex-induced lung injury, and not associated with reductions in neutrophil accumulation as measured by extraction from tissues of myeloperoxidase. The results demonstrate immune complex induced vascular injury induced by activated macrophage was inhibited effectively by L-NIO as a potent inhibitor [1].

In vivo: A dose-dependent increase in mean systemic arterial blood pressure accompanied by bradycardia was induced at the administration of L-NIO (0.03-300 mgkg-1, i.v.). L-NIO (100 mgkg-, i.v.) has an effect inhibitor of the hypotensive responses to ACh and bradykinin. L-arginine (30-100 mg kg- 1, i.v.) in a dose-dependent manner reversed the increase in blood pressure and bradycardia produced by these compounds. It was enantiomer specific for all of these effects. These facts indicate that L-NIO can inhibit NO synthase as inhibitors in the vascular endothelium and verify that L-NIO plays an important role for NO synthesis in the maintenance of vascular tone and blood pressure [2].

Clinical trial: So far, no clinical study has been conducted.

References:
[1].  Mulligan MS, Moncada S, Ward PA. Protective effects of inhibitors of nitric oxide synthase in immune complex-induced vasculitis. Br J Pharmacol. 1992 Dec;107(4):1159-62.
[2].  Rees DD, Palmer RM, Schulz R, Hodson HF, Moncada S.Br J Pharmacol. Characterization of three inhibitors of endothelial nitric oxide synthase in vitro and in vivo.1990 Nov;101 (3):746-52.

Chemical Properties

Cas No. 159190-44-0 SDF
Chemical Name (S,E)-2-amino-5-((1-aminoethylidene)amino)pentanoic acid dihydrochloride
Canonical SMILES OC([C@H](CCC/N=C(C)/N)N)=O.Cl.Cl
Formula C7H15N3O2.2HCl M.Wt 246.13
Solubility <24.61mg/ml in Water Storage Desiccate at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 4.0629 mL 20.3145 mL 40.6289 mL
5 mM 0.8126 mL 4.0629 mL 8.1258 mL
10 mM 0.4063 mL 2.0314 mL 4.0629 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 5 reference(s) in Google Scholar.)

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