L6H21 |
Catalog No.GC49560 |
L6H21, a Chalcone derivative, is an orally active, potent and specific myeloid differentiation 2 (MD-2) inhibitor.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 24533-47-9
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
L6H21 is an inhibitor of myeloid differentiation 2 (MD-2), also known as lymphocyte antigen 96 (LY96).1 It binds to MD-2 (Kd = 33.3 µM in a cell-free assay) and inhibits the protein-protein interaction between MD-2 and toll-like receptor 4 (TLR4) in LPS-stimulated ECV304 cells. L6H21 (10 µM) decreases the migration of LPS-stimulated HCT116 colon cancer cells.2 It inhibits LPS-induced increases in the production of TNF-α and IL-6 in mouse primary macrophages (IC50s = 6.17 and 7.72 µM, respectively), as well as increases survival in a mouse model of LPS-induced sepsis.1 L6H21 (40 mg/kg) increases cardiac levels of glutathione peroxidase 4 (GPX4) and decreases cardiac mitochondrial reactive oxygen species (ROS) in rats fed a high-fat diet.3
1.Wang, Y., Shan, X., Chen, G., et al.MD-2 as the target of a novel small molecule, L6H21, in the attenuation of LPS-induced inflammatory response and sepsisBr. J. Pharmacol.172(17)4391-4405(2015) 2.Rajamanickam, V., Yan, T., Xu, S., et al.Selective targeting of the TLR4 co-receptor, MD2, prevents colon cancer growth and lung metastasisInt. J. Biol. Sci.16(8)1288-1302(2020) 3.Sumneang, N., Oo, T.T., Singhanat, K., et al.Inhibition of myeloid differentiation factor 2 attenuates cardiometabolic impairments via reducing cardiac mitochondrial dysfunction, inflammation, apoptosis and ferroptosis in prediabetic ratsBiochem. Biophys. Acta Mol. Basis Dis1868(2)166301(2021)
Cas No. | 24533-47-9 | SDF | Download SDF |
Canonical SMILES | COC1=C(C=CC=C1OC)/C=C/C(C2=CC=C(C=C2)OC)=O | ||
Formula | C18H18O4 | M.Wt | 298.3 |
Solubility | DMF: 20 mg/ml,DMF:PBS (pH 7.2) (1:4): 0.20 mg/ml,DMSO: 5 mg/ml | Storage | -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 3.3523 mL | 16.7616 mL | 33.5233 mL |
5 mM | 0.6705 mL | 3.3523 mL | 6.7047 mL |
10 mM | 0.3352 mL | 1.6762 mL | 3.3523 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
(Based on Reviews and 6 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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