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LDN 193189 dihydrochloride (Synonyms: DM-3189)

Catalog No.GC50327

Potent and selective ALK2 and ALK3 inhibitor; promotes neural induction of hPSCs

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LDN 193189 dihydrochloride Chemical Structure

Cas No.: 1435934-00-1

Size Price Stock Qty
10 mg
$333.00
In stock
50 mg
$1,338.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product has been cited by 1 publications

Product Documents

Quality Control & SDS

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Background

Potent and selective ALK2 and ALK3 inhibitor (IC50 values are 5 and 30 nM, respectively); inhibits BMP4-mediated Smad1/5/8 activation. Exhibits >200-fold selectivity for BMP signaling over TGF-β signaling. Also exhibits selectivity over AMPK, PDGFR and MAPK signaling. Promotes neural induction of hPSCs in combination with SB 431542 (Cat.No. 1614). Also induces differentiation of hPSCs into nociceptive sensory neurons in combination with SB 431542 (Cat.No. 1614), SU 5402 (Cat.No.3300), CHIR 99021 (Cat.No. 4423) and DAPT (Cat.No. 2634).

Yu et al (2008) BMP type I receptor inhibition reduces heterotopic ossification. Nat.Med. 14 1363 PMID:19029982 |Chambers et al (2012) Combined small-molecule inhibition accelerates developmental timing and converts human pluripotent stem cells into nociceptors. Nat.Biotechnol. 30 715 PMID:22750882 |Cuny et al (2008) Structure-activity relationship study of bone morphogenetic protein (BMP) signaling inhibitors. Bioorg.Med.Chem.Lett. 18 4388 PMID:18621530 |Li et al (2013) Chemical approaches to stem cell biology and therapeutics. Cell Stem Cell 13 270 PMID:24012368 |Lancaster et al (2015) Generation of Cerebral Organoids from Human Pluripotent Stem Cells Nat. Protoc. 9 2329 PMID:25188634 |Kadoshima et al (2013) Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex. Proc.Natl.Acad.Sci.USA. 110 20284 PMID:24277810 |Dye et al (2015) In vitro generation of human pluripotent stem cell derived lung organoids. Elife 4 PMID:25803487 |Wimmer et al (2019) Human blood vessel organoids as a model of diabetic vasculopathy. Nature 565 505 PMID:30651639

Chemical Properties

Cas No. 1435934-00-1 SDF
Synonyms DM-3189
Canonical SMILES N1(C2=CC=C(C3=CN4N=CC(C5=CC=NC6=C5C=CC=C6)=C4N=C3)C=C2)CCNCC1.Cl.Cl
Formula C25H22N6.2HCl M.Wt 479.4
Solubility DMF: 1 mg/ml,DMSO: 2 mg/ml Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 2.0859 mL 10.4297 mL 20.8594 mL
5 mM 0.4172 mL 2.0859 mL 4.1719 mL
10 mM 0.2086 mL 1.043 mL 2.0859 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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Review for LDN 193189 dihydrochloride

Average Rating: 5 ★★★★★ (Based on Reviews and 32 reference(s) in Google Scholar.)

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