GMax™ LiposomeRNA Transfection Reagent |
| Catalog No.GK20007 |
GMax™ LiposomeRNA Transfection Reagent can obtain the highest transfection efficiency in a variety of cell types, and is suitable for siRNA-mediated gene suppression experiments.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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GMax™ LiposomeRNA Transfection Reagent can obtain the highest transfection efficiency in a variety of cell types, and is suitable for siRNA-mediated gene suppression experiments. GMax™ LiposomeRNA is a unique RNAi-specific cationic liposome transfection reagent, suitable for delivering siRNA and miRNA to all cell types.
• Ultra-high transfection efficiency, and low RNAi concentration required, better gene suppression effect, few non-specific effects.
• Low cytotoxicity, easy to optimize, showing low cytotoxicity across the 10-fold transfection reagent concentration range.
• Excellent transfection efficiency for miRNA antagonists and mimics.
• It can be used in a variety of cell types and is the most versatile method suitable for various gene suppression experiments.
• Simple and fast experimental protocol to obtain stable and reproducible results.
Preparation Before Experiment
• siRNA or miRNA (10 μM stock)
• Opti-MEM Reduced Serum Medium
• Microcentrifuge tube
Experimental Steps
A. GMax™ LiposomeRNA Transfection Reagent requirements
| Component | 96-well | 24-well | 6-well |
| Final siRNA per well | 1 pmol | 5 pmol | 25 pmol |
| Final GMax™ LiposomeRNA Reagent per well | 0.3 μL | 1.5 μL | 7.5 μL |
B. Transfection of siRNA
Reverse transfection is faster to perform than forward transfection and is themethod of choice for high-throughput transfection. Perform reverse transfection by preparing complexes inside the wells,and then adding cells and medium. Because the cells and siRNA-lipid complexes are prepared on the same day, we recommended using 2.5× more cells than for a regular transfection method.
C. GMax™ LiposomeRNA Transfection Reagent Protocol
Transfect cells according to the following table.The transfection is designed for one RNA amount combined with one amount of GMax™ LiposomeRNA. The prepared mix is enough to have triplicates (96-well), duplicates (24-well), and single well (6-well) transfections, and account for pipetting variations. For additional information on scaling your transfection reaction.
| Components | 0.25 mL | 0.5 mL |
| GMax™ LiposomeRNA Transfection Reagent | 0.25 mL | 0.5 mL |
| Store at 4°C. Do not freeze. | ||
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Average Rating: 5 (Based on Reviews and 5 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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