Lucifer Yellow CH (Synonyms: Lucifer Yellow carbohydrazide) |
| Catalog No.GC44089 |
Lucifer Yellow CH is a water-soluble, high-intensity fluorescent dye with maximum excitation/emission at 430nm/540nm, respectively.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 67769-47-5
Sample solution is provided at 25 µL, 10mM.
Lucifer Yellow CH is a water-soluble, high-intensity fluorescent dye with maximum excitation/emission at 430nm/540nm, respectively. Lucifer Yellow CH contains a carbodihydrazide (CH) group that can covalently bind to surrounding biomolecules during aldehyde fixation. Lucifer Yellow CH is impermeable to cell membranes at physiological pH and can be loaded into living cells by microinjection, pinocytosis, scraping, or ATP-induced permeabilization. Lucifer Yellow CH can be used as a biological tracer to label neural cells, monitor neuronal branching, regeneration, gap junction detection and characterization, and selective ablation of cells [1-5].
References:
[1] Stewart WW. Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer. Cell. 1978 Jul;14(3):741-59.
[2] Klein M, et al. Transport of lucifer yellow CH into plant vacuoles--evidence for direct energization of a sulphonatedsubstance and implications for the design of new molecular probes. FEBS Lett. 1997 Dec 22;420(1):86-92.
[3] Takeuchi K, et al. Effect of superoxide derived from lucifer yellow CH on voltage-gated currents of mouse taste budcells. Chem Senses. 2008 Jun;33(5):425-32.
[4] Sun Y, Leong N T, Jiang T, et al. Switch-like Arp2/3 activation upon WASP and WIP recruitment to an apparent threshold level by multivalent linker proteins in vivo[J]. Elife, 2017, 6: e29140.
[5] Zhang Y, Hou M C, Li J J, et al. Cardiac β‐adrenergic receptor activation mediates distinct and cell type‐dependent changes in the expression and distribution of connexin 43[J]. Journal of Cellular and Molecular Medicine, 2020, 24(15): 8505-8517.
This protocol provides a guide only and should be modified to suit your specific needs.
1. Preparation of staining solution
(1) Preparation of storage solution: Dissolve 1mg of Lucifer Yellow CH in 1mL of ddH2O to prepare a 1mg/mL storage solution.
Note: Store unused storage solution at -20°C or -80°C away from light and avoid repeated freezing and thawing.
(2) Prepare the working solution: dilute the stock solution with a suitable buffer (such as preheated serum-free cell culture medium) to prepare a 0.05-0.1% Lucifer Yellow CH working solution.
Note: Please adjust the working solution concentration according to the actual situation and prepare it before use.
2. Cell Staining
Suspension cells: Collect cells by centrifugation, discard the supernatant, and wash twice with PBS, 5 minutes each time.
Adherent cells: Wash twice with PBS, add trypsin to digest the cells, and centrifuge at 1000g for 3-5 minutes after digestion.
(1) Add 1mL of Lucifer Yellow CH working solution to the collected cells and incubate at room temperature for 5-30 minutes.
(2) Centrifuge at 400g, 4°C for 3-4 minutes and discard the supernatant.
(3) Wash twice with PBS, 5 minutes each time.
(4) Resuspend the cells in 1mL of serum-free medium or PBS and examine under a fluorescence microscope or flow cytometer.
Notes：
1. Fluorescent dyes all have quenching problems. Please try to avoid light to slow down fluorescence quenching
2. For your safety and health, please wear a lab coat and disposable gloves when operating.
| Cas No. | 67769-47-5 | SDF | |
| Synonyms | Lucifer Yellow carbohydrazide | ||
| Chemical Name | 6-amino-2-[(hydrazinylcarbonyl)amino]-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinoline-5,8-disulfonic acid, dilithium salt | ||
| Canonical SMILES | O=C(C1=CC(S([O-])(=O)=O)=C(N)C2=C1C3=CC(S([O-])(=O)=O)=C2)N(NC(NN)=O)C3=O.[Li+].[Li+] | ||
| Formula | C13H9N5O9S2•2Li | M.Wt | 457.2 |
| Solubility | 1mg/mL in water | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.1872 mL | 10.9361 mL | 21.8723 mL |
| 5 mM | 437.4 μL | 2.1872 mL | 4.3745 mL |
| 10 mM | 218.7 μL | 1.0936 mL | 2.1872 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >80.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 15 reference(s) in Google Scholar.)
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