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Malachite Green (chloride) (Synonyms: Basic Green 4, C.I. 4200, C.I. 42000)

Catalog No.GC52358

A triphenylmethane dye

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Malachite Green (chloride) Chemical Structure

Cas No.: 569-64-2

Size Price Stock Qty
10 mg
$43.00
In stock
25 mg
$195.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

Malachite green is a triphenylmethane dye.1 It inhibits the proliferation of HEp-2 human larynx cells (IC50 = 2.06 µM). Malachite green is cytotoxic to HEp-2 cells and Caco-2 human colorectal adenocarcinoma cells (IC50s = 2.03 and 13.8 µM, respectively). It reduces fungal infection in, and increases hatching rates of, rainbow trout eggs when used at a concentration of 2 µg/ml for 60 minutes twice a week.2 Malachite green has been used as a biochemical indicator dye for titrations, pH indicators, and counterstains in histology applications.3,4,5 Formulations containing malachite green have been used in the textile and pigment industries as dyes.

1.Stammati, A., Nebbia, C., De Angelis, I., et al.Effects of malachite green (MG) and its major metabolite, leucomalachite green (LMG), in two human cell linesToxicol. In Vitro19(7)853-858(2005) 2.Kitancharoen, N., Yamamoto, A., and Hatai, K.Effects of sodium chloride, hydrogen peroxide and malachite green on fungal infection in rainbow trout eggsBiocontrol Sci.3(2)113-115(1998) 3.Tuthill, S.M., Kolling, O.W., and Roberts, K.H.Photometric and visual titration of certain alkaloids in glacial acetic acid using malachite green as indicatorAnal. Chem.32(12)1678-1681(1960) 4.Decnop-Weever, L.G., and Kraak, J.C.Determination of sulphite in wines by gas-diffusion flow injection analysis utilizing spectrophotometric pH-detectionAnalytica Chimica Acta337125-131(1997) 5.Dutton, R.J., Bitton, G., and Koopman, B.Malachite green-INT (MINT) method for determining active bacteria in sewageAppl. Environ. Microbiol.46(6)1263-1267(1983)

Chemical Properties

Cas No. 569-64-2 SDF Download SDF
Synonyms Basic Green 4, C.I. 4200, C.I. 42000
Canonical SMILES CN(C)C(C=C1)=CC=C1/C(C2=CC=CC=C2)=C3C=C/C(C=C/3)=[N+](C)/C.[Cl-]
Formula C23H25N2Cl M.Wt 364.9
Solubility DMF: 1 mg/ml,DMSO: 1 mg/ml,Ethanol: 0.5 mg/ml,PBS (pH 7.2): 0.25 mg/ml Storage -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 2.7405 mL 13.7024 mL 27.4048 mL
5 mM 0.5481 mL 2.7405 mL 5.481 mL
10 mM 0.274 mL 1.3702 mL 2.7405 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 30 reference(s) in Google Scholar.)

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