MDL 72527 dihydrochloride |
| Catalog No.GC10545 |
polyamine oxidase (POA) inhibitor
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 93565-01-6
Sample solution is provided at 25 µL, 10mM.
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MDL 72527 is a polyamine oxidase (PAO) inhibitor that inhibits spermine oxidase and N1-acetylpolyamine oxidase (IC50s = 6.1 and 0.02 µM, respectively).[1] It does not inhibit monoamine oxidase or D-amino acid oxidase. MDL 72527 inhibits PAO-dependent H2O2 production in vitro and in vivo and is used to study the role of PAO activity in diverse models, including infection, cancer, and hyperoxia.[2][3][4]
Reference:
[1]. Moriya, S.S., Miura, T., Takao, K., et al. Development of irreversible inactivators of spermine oxidase and N1-acetylpolyamine oxidase. Biol. Pharm. Bull. 37(3), 475-480 (2014).
[2]. Chaturvedi, R., Cheng, Y., Asim, M., et al. Induction of polyamine oxidase 1 by Helicobacter pylori causes macrophage apoptosis by hydrogen peroxide release and mitochondrial membrane depolarization. J. Biol. Chem. 279(38), 40161-40173 (2004).
[3]. Goodwin, A.C., Destefano, C.E., Wu, S., et al. Polyamine catabolism contributes to enterotoxigenic Bacteroides fragilis-induced colon tumorigenesis. Proc. Natl. Acad. Sci. USA 108(37), 15354-15359 (2011).
[4]. Patel, C.A., Xu, Z., Xing, J., et al. Treatment with polyamine oxidase inhibitor reduces microglial activation and limits vascular injury in ischemic retinopathy. Biochim Biophys. Acta. 1862(9), 1628-1639 (2016).
| Cas No. | 93565-01-6 | SDF | |
| Chemical Name | N1,N4-di(buta-2,3-dien-1-yl)butane-1,4-diamine dihydrochloride | ||
| Canonical SMILES | C=C=CCNCCCCNCC=C=C.Cl.Cl | ||
| Formula | C12H20N2.2HCl | M.Wt | 265.23 |
| Solubility | DMF: slightly,DMSO: 0.33 mg/ml,PBS (pH 7.2): 10 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.7703 mL | 18.8516 mL | 37.7031 mL |
| 5 mM | 0.7541 mL | 3.7703 mL | 7.5406 mL |
| 10 mM | 0.377 mL | 1.8852 mL | 3.7703 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >97.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 27 reference(s) in Google Scholar.)
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