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MeOSuc-Ala-Ala-Pro-Val-AMC (Synonyms: I 1270, MeOSucAAPVAMC, Methoxy SuccinylAlaAlaProValAMC)

Catalog No.GC44157

MeOSuc-Ala-Ala-Pro-Val-AMC is a fluorogenic substrate for human leukocyte and porcine pancreatic elastase (Km: 362 μM, Ex=380 nm, Em=460 nm).

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MeOSuc-Ala-Ala-Pro-Val-AMC Chemical Structure

Cas No.: 72252-90-5

Size Price Stock Qty
1mg
$26.00
In stock
5mg
$78.00
In stock
10mg
$111.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

MeOSuc-Ala-Ala-Pro-Val-AMC (MeOSuc-AAPV-AMC) is a fluorogenic substrate for human leukocyte and porcine pancreatic elastase. The methoxy succinyl (MeO-Suc) peptide AAPV is avidly hydrolyzed by these elastases but not by cathepsin G. The addition of 7-amino-4-methylcoumarin (AMC) generates a peptide of low fluorescence (excitation: 355-380 nm; emission 440-460 nm) that demonstrates a linear increase in fluorescence in the presence of elastase. This peptidyl-AMC substrate detects as little as 11 pM human leukocyte elastase and 18 pM porcine pancreatic elastase.

Chemical Properties

Cas No. 72252-90-5 SDF
Synonyms I 1270, MeOSucAAPVAMC, Methoxy SuccinylAlaAlaProValAMC
Canonical SMILES COC(CCC(N[C@H](C(N[C@H](C(N1CCC[C@H]1C(N[C@@H](C(C)C)C(NC2=CC=C(C(C)=CC(O3)=O)C3=C2)=O)=O)=O)C)=O)C)=O)=O
Formula C31H41N5O9 M.Wt 627.7
Solubility DMF: 20 mg/ml,DMF:PBS(pH 7.2)(1:1): 0.5 mg/ml,DMSO: 16 mg/ml Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 1.5931 mL 7.9656 mL 15.9312 mL
5 mM 0.3186 mL 1.5931 mL 3.1862 mL
10 mM 0.1593 mL 0.7966 mL 1.5931 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 15 reference(s) in Google Scholar.)

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