MSDC-0160 (Synonyms: Mitoglitazone; CAY10415) |
| Catalog No.GC15936 |
mTOT-modulating insulin sensitizer
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 146062-49-9
Sample solution is provided at 25 µL, 10mM.
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MSDC-0160 is a prototype mTOT-modulating insulin sensitizer [1].
MSDC-0160 is a thiazolidinedione insulin sensitizer without the peroxisome proliferator–activated receptor-γ (PPAR-γ)-dependent side effects. Co-incubation of islets with MSDC-0160 and IGF-1 reduces the resistance of insulin-signaling pathway and preserves insulin content. MSDC-0160 at 50μM alone also prevents the loss of insulin content significantly. Besides that, treatment of MSDC-0160 increases the phosphorylation of AMPK and reduces mTOR phosphorylation. Moreover, MSDC-0160 is also found to display pro-survival effects in human islets via reducing the level of cleaved caspase-3 and increasing the expression of apoptosis-related genes such as bcl2. In addition, MSDC-0160 exerts prevention of nuclear translocation with β-catenin localized on the cell membrane or cytoplasm in human β-cells [1].
References:
[1] Rohatgi N, Aly H, Marshall C A, et al. Novel insulin sensitizer modulates nutrient sensing pathways and maintains β-cell phenotype in human islets. PloS one, 2013, 8(5): e62012.
| Cell experiment [1]: | |
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Cell lines |
LUHMES cells |
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Preparation method |
Limited solubility. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
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Reacting condition |
10 or 100 μM, 1 hour |
|
Applications |
MSDC-0160 (10 μM) pretreatment (1 hour) prevented the MPP+ (10 μM)-induced loss of both tyrosine hydroxylase (TH)-immunoreactive differentiated Lund human mesencephalic (LUHMES) cells. MSDC-0160 protected TH-immunoreactive neurons. MSDC-0160 counteracted both MPP+-induced shortening of neurite length and reduced branching in both LUHMES cells. MSDC-0160 (10 or 100 μM) prevented the loss of GFP-fluorescent dopaminergic neurons induced by MPP+ (0.75 mM) in nematodes. MSDC-0160 (10 μM) blocked LPS-induced increases in iNOS expression in BV2 cell lysates. |
| Animal experiment [1]: | |
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Animal models |
MPTP mouse model of Parkinson’s disease (PD), En1+/- genetic mouse model of PD |
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Dosage form |
Oral administration, 30 mg/kg per day |
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Application |
MSDC-0160 (30 mg/kg per day, p.o.) treatment for 3 days after MPTP injection improved motor behavior, protects nigrostriatal neurons, and suppressed disease progression in the MPTP mouse model of Parkinson’s disease (PD). MSDC-0160 improved motor behavior in the open-field and rotarod tests and prevented dopaminergic neurodegeneration in the En1+/- genetic mouse model of PD. MSDC-0160 modulated mTOR signaling in C. elegans and the MPTP mouse model of PD. MSDC-0160 down-regulated mTOR signaling and restored autophagy in the En1+/- genetic mouse model of PD. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1]. Rohatgi N, Aly H, Marshall C A, et al. Novel insulin sensitizer modulates nutrient sensing pathways and maintains β-cell phenotype in human islets. PloS one, 2013, 8(5): e62012. | |
| Cas No. | 146062-49-9 | SDF | |
| Synonyms | Mitoglitazone; CAY10415 | ||
| Canonical SMILES | CCC1=CN=C(C=C1)C(=O)COC2=CC=C(C=C2)CC3C(=O)NC(=O)S3 | ||
| Formula | C19 H18 N2 O4 S | M.Wt | 370.42 |
| Solubility | ≥ 37mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.6996 mL | 13.4982 mL | 26.9964 mL |
| 5 mM | 0.5399 mL | 2.6996 mL | 5.3993 mL |
| 10 mM | 0.27 mL | 1.3498 mL | 2.6996 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 7 reference(s) in Google Scholar.)
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