N-methyl Mesoporphyrin IX |
| Catalog No.GC44416 |
A ferrochelatase inhibitor and turn-on biosensor for DNA
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 142234-85-3
Sample solution is provided at 25 µL, 10mM.
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N-methyl Mesoporphyrin IX is a transition state analog of porphyrin and an inhibitor of ferrochelatase.[1] It inhibits ferrochelatase in mouse liver mitochondria in vitro and in vivo, leading to an accumulation of porphyrin in isolated liver mitochondria following administration of an intravenous dose of 80 nmol. N-methyl Mesoporphyrin IX has been used as a turn-on biosensor for target DNA sequences when used in complex with a G-quadruplex-forming sequence fused to a DNA sequence complementary to the target sequence.[2] It has also been used to detect amyloid-β (1-40) (Aβ40) fibrils in vitro and in live PC12 cells overexpressing Aβ.[3] N-methyl Mesoporphyrin displays excitation/emission maxima of 399/610 nm, respectively.[4]
Reference:
[1]. de Matteis, F., Gibbs, A.H., and Smith, A.G. Inhibition of protohaem ferro-lyase by N-substituted porphyrins. Structural requirements for the inhibitory effect. Biochem. J. 189(3), 645-648 (1980).
[2]. Ren, J., Qin, H., Wang, J., et al. Label-free detection of nucleic acids by turn-on and turn-off G-quadruplex-mediated fluorescence. Anal. Bioanal. Chem. 399(8), 2763-2770 (2011).
[3]. Li, M., Zhao, A., Ren, J., et al. N‑Methyl mesoporphyrin IX as an effective probe for monitoring Alzheimer’s disease β‑amyloid aggregation in living cells. ACS Chem. Neurosci. 8(6), 1299-1304 (2017).
[4]. Paramasivan, S., and Bolton, P.H. Mix and measure fluorescence screening for selective quadruplex binders. Nucleic Acids Res. 36(17), e106 (2008).
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Review for N-methyl Mesoporphyrin IX
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