Oil Red O (Synonyms: ORO) |
| Catalog No.GC25676 |
Oil Red O is a red neutral lipid droplet stain.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1320-06-5
Sample solution is provided at 25 µL, 10mM.
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Oil Red O is a type of lysochrome diazo dye, also known as Solvent Red 27, Sudan Red 5B. It is commonly used for staining neutral triglycerides and lipids on frozen sections, as well as certain lipoproteins on paraffin sections. Oil Red O is in the form of a red powder and exhibits a peak absorbance at 518 nm [1].
Reference:
[1]. Kraus NA, Ehebauer F, Zapp B, Rudolphi B, Kraus BJ, Kraus D. Quantitative assessment of adipocyte differentiation in cell culture. Adipocyte. 2016 Oct 1;5(4):351-8.
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1. Oil Red O Staining Preparation 1.1 Oil Red O solution stock: Dissolve 0.5 g Oil Red O in 200 ml isopropyl alcohol at 56 ℃ for 1 h. Cool down before further use. Store at room temperature. 1.2 Oil Red O working solution: Make fresh the day of use. Mix four parts distilled water with six parts of stock solution. Let solution rest for at least 15 min. Precipitate will form. Filter solution through a filter paper, for example, 3MM Chr Whatman paper. Keep at room temperature until use. 1.3 1×PBS, pH 7.4. 1.4 60% (v/v) isopropyl alcohol in distilled water. 2. Fixing cells 2.1 Wash cells very carefully with PBS as they are easy to detach from the plate. 2.2 Add 1 ml of 4% PFA per well and incubate for 20 min at room temperature on a level surface with no shaking or tilting. 2.3 Discard the PFA in a hazardous waste chamber and wash cells twice for 5 min in PBS. 3. Mounting of cover slips 3.1 Add a 40 ml drop of mounting solution in the middle of a glass slide. 3.2 Remove the cover slip from the six-well plate using tweezers and dry the edges by blotting with tissue paper. Then place the cover slip on the mounting solution (side with cells facing down) taking care not to trap bubbles. 3.3 Incubate 2 h at room temperature in the dark to let the mounting solution solidify. 3.4 Wipe off any precipitate on the cover slip with a moist tissue paper. 3.5 Keep the slides in the dark at 4 ℃. 4. Oil Red O staining 4.1 Fix cells and remove PBS (see Section 2). 4.2 Wash cells with 60% isopropyl alcohol. 4.3 Add 1.5 ml of Oil Red O working solution per well and incubate for 15–30 min. Observe under microscope until cells are properly stained. Make sure to stop the staining when/if precipitate starts to form. Wash cells with 60% isopropyl alcohol. 4.4 Wash cells with 1 ml PBS and leave in PBS until further use. 4.5 Mount cover slips on glass slides or use the stained cells for quantification by Oil Red O extraction. The Oil Red O-stained cells can be photographed directly, or higher magnification images of cells on the mounted cover slips can be obtained using any microscope equipped with an RGB camera.
This protocol only provides a guideline, and should be modified according to your specific needs.
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| Cas No. | 1320-06-5 | SDF | |
| Synonyms | ORO | ||
| Formula | C26H24N4O | M.Wt | 408.49 |
| Solubility | DMSO : 4.17 mg/mL (10.21 mM; ultrasonic and warming and heat to 80°C) | Storage | Store at -20°C, protect from light, stored under nitrogen |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.448 mL | 12.2402 mL | 24.4804 mL |
| 5 mM | 0.4896 mL | 2.448 mL | 4.8961 mL |
| 10 mM | 0.2448 mL | 1.224 mL | 2.448 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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