(-)-p-Bromotetramisole Oxalate (Synonyms: (-)-p-Bromotetramisole, L-para-Bromotetramisole) |
| Catalog No.GC14520 |
(-)-p-Bromotetramisole Oxalate(L-p-bromotetramisole) is a potent and non-specific inhibitor of alkaline phosphatase.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 62284-79-1
Sample solution is provided at 25 µL, 10mM.
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(-)-p-Bromotetramisole Oxalate(L-p-bromotetramisole) is a potent and non-specific inhibitor of alkaline phosphatase [1-2].
(-)-p-Bromotetramisole Oxalate (1 mM) augmented cAMP-stimulated iodide efflux and, by itself, stimulated a larger efflux than that evoked by cAMP agonists, suggesting that it may promote the opening of humancystic fibrosis transmembrane conductance regulator (CFTR) in CHO cells[3]. In PC12 cells, (-)-p-Bromotetramisole Oxalate (0.3 mM) enhances ionomycin-stimulated norepinephrine (NA) release[4].
(-)-p-Bromotetramisole Oxalate(30 mg/kg; ivgtt) significantly inhibited norepinephrine-induced MABP elevation and renal vascular resistance. It reduces renal vascular and blood pressure response to norepinephrine[5]. Systemic infusion of (-)-p-Bromotetramisole Oxalate (0.8 ml/min of 10 mM I-p-Bromotetramisole oxalate; ivgtt) increases Fractional excretion of phosphate (FEPi) in Sprague-Dawley rats[6].
References:
[1]. Borgers M. The cytochemical application of new potent inhibitors of alkaline phosphatases. J Histochem Cytochem. 1973 Sep;21(9):812-24. doi: 10.1177/21.9.812. PMID: 4741290.
[2]. Borgers M, Thoné F. The inhibition of alkaline phosphatase by L-p-bromotetramisole. Histochemistry, 1975, 44(3): 277-280.
[3]. Lansdell KA, Kidd JF, et,al. Regulation of murine cystic fibrosis transmembrane conductance regulator Cl- channels expressed in Chinese hamster ovary cells. J Physiol. 1998 Nov 1;512 ( Pt 3)(Pt 3):751-64. doi: 10.1111/j.1469-7793.1998.751bd.x. PMID: 9769419; PMCID: PMC2231228.
[4]. Kitamura T, Murayama T, et,al. Enhancement of Ca2+-induced noradrenaline release by vanadate in PC12 cells: possible involvement of tyrosine phosphorylation. Brain Res. 2000 Jan 31;854(1-2):165-71. doi: 10.1016/s0006-8993(99)02299-4. PMID: 10784118.
[5]. Jackson EK, Zhang Y,et,al. Alkaline Phosphatase Inhibitors Attenuate Renovascular Responses to Norepinephrine. Hypertension. 2017 Mar;69(3):484-493. doi: 10.1161/HYPERTENSIONAHA.116.08623. Epub 2017 Jan 30. PMID: 28137984; PMCID: PMC5310812.
[6]. Onsgard-Meyer M, McCoy AL, et,al. Effect of bromotetramisole on renal phosphate excretion. Proc Soc Exp Biol Med. 1996 Nov;213(2):193-5. doi: 10.3181/00379727-213-44050. PMID: 8931664.
| Cell experiment [1]: | |
|
Cell lines |
PC12 cells |
|
Preparation Method |
The prelabeled PC12 cells were incubated with vehicle or 5 µM ionomycin in the presence of 2 mM CaCl2. (-)-p-Bromotetramisole Oxalate (BTO) was further added to the assay mixture. |
|
Reaction Conditions |
0.3 mM |
|
Applications |
(-)-p-Bromotetramisole Oxalate (0.3 mM) enhances ionomycin-stimulated norepinephrine (NA) release. |
| Animal experiment [2]: | |
|
Animal models |
Sprague-Dawley rats |
|
Preparation Method |
Fractional excretion of phosphate (FEPi) was measured in thyroparathyroidectomized rats before and during a systemic infusion at 0.8 ml/min of 10 mM (-)-p-Bromotetramisole Oxalate. |
|
Dosage form |
0.8 ml/min of 10 mM (-)-p-Bromotetramisole Oxalate; ivgtt |
|
Applications |
Systemic infusion of (-)-p-Bromotetramisole Oxalate increases Fractional excretion of phosphate (FEPi) in rats. |
|
References: [1]. Kitamura T, Murayama T, et,al. Enhancement of Ca2+-induced noradrenaline release by vanadate in PC12 cells: possible involvement of tyrosine phosphorylation. Brain Res. 2000 Jan 31;854(1-2):165-71. doi: 10.1016/s0006-8993(99)02299-4. PMID: 10784118. |
|
| Cas No. | 62284-79-1 | SDF | |
| Synonyms | (-)-p-Bromotetramisole, L-para-Bromotetramisole | ||
| Canonical SMILES | BrC(C=C1)=CC=C1[C@H]2N=C3SCCN3C2.OC(C(O)=O)=O | ||
| Formula | C13H13BrN2O4S | M.Wt | 373.22 |
| Solubility | ≥ 18.65mg/mL in DMSO | Storage | Desiccate at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.6794 mL | 13.3969 mL | 26.7938 mL |
| 5 mM | 0.5359 mL | 2.6794 mL | 5.3588 mL |
| 10 mM | 0.2679 mL | 1.3397 mL | 2.6794 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
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- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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