Home>>p-Nitrobenzyl mesylate
p-Nitrobenzyl mesylate Catalog No.GC44663

Size Price Stock Qty
500μg
$44.00
In stock
1mg
$79.00
In stock
5mg
$309.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Chemical Properties

Cas No. 39628-94-9 SDF Download SDF
Synonyms 4-Nitrobenzyl mesylate
Chemical Name 4-nitro-benzenemethanol, 1-methanesulfonate
Canonical SMILES CS(OCC1=CC=C([N+]([O-])=O)C=C1)(=O)=O
Formula C8H9NO5S M.Wt 231.2
Solubility 100mM in DMSO Storage Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
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Background

p-Nitrobenzyl mesylate (PNBM) is a reagent that is used to alkylate thiophosphates, forming thiophosphate ester epitopes that can be recognized by specific antibodies.[1] Substrates phosphorylated with adenosine 5'-(γ-thio)-triphosphate or N6-benzyl-ATPγS can be alkylated by PNBM.[2],[3] The tagged substrates can be isolated by immunoprecipitation or immunoaffinity purification, or they can be detected by immunoblotting.

Reference:
[1]. Allen, J.J., Li, M., Brinkworth, C.S., et al. A semisynthetic epitope for kinase substrates. Nat. Methods 4(6), 511-516 (2007).
[2]. Kumar, V., Weng, Y.-C., Geldenhuys, W.J., et al. Generation and characterization of ATP analog-specific protein kinase Cδ. J. Biol. Chem. 290(4), 1936-1951 (2015).
[3]. Leissing, F., Nomoto, M., Bocola, M., et al. Substrate thiophosphorylation by Arabidopsis mitogen-activated protein kinases. BMC Plant Biol. 16(48), (2016).