|p-Nitrobenzyl mesylate Catalog No.GC44663|
Sample solution is provided at 25 µL, 10mM.
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|Cas No.||39628-94-9||SDF||Download SDF|
|Chemical Name||4-nitro-benzenemethanol, 1-methanesulfonate|
|Solubility||100mM in DMSO||Storage||Store at -20°C|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Shipping Condition||Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
p-Nitrobenzyl mesylate (PNBM) is a reagent that is used to alkylate thiophosphates, forming thiophosphate ester epitopes that can be recognized by specific antibodies. Substrates phosphorylated with adenosine 5'-(γ-thio)-triphosphate or N6-benzyl-ATPγS can be alkylated by PNBM., The tagged substrates can be isolated by immunoprecipitation or immunoaffinity purification, or they can be detected by immunoblotting.
. Allen, J.J., Li, M., Brinkworth, C.S., et al. A semisynthetic epitope for kinase substrates. Nat. Methods 4(6), 511-516 (2007).
. Kumar, V., Weng, Y.-C., Geldenhuys, W.J., et al. Generation and characterization of ATP analog-specific protein kinase Cδ. J. Biol. Chem. 290(4), 1936-1951 (2015).
. Leissing, F., Nomoto, M., Bocola, M., et al. Substrate thiophosphorylation by Arabidopsis mitogen-activated protein kinases. BMC Plant Biol. 16(48), (2016).