p-Nitrobenzyl mesylate (Synonyms: 4-Nitrobenzyl mesylate) |
| Catalog No.GC44663 |
p-Nitrobenzyl mesylate (PNBM) is a reagent that is used to alkylate thiophosphates, forming thiophosphate ester epitopes that can be recognized by specific antibodies.
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Cas No.: 39628-94-9
Sample solution is provided at 25 µL, 10mM.
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p-Nitrobenzyl mesylate (PNBM) is a reagent that is used to alkylate thiophosphates, forming thiophosphate ester epitopes that can be recognized by specific antibodies.[1] Substrates phosphorylated with adenosine 5'-(γ-thio)-triphosphate or N6-benzyl-ATPγS can be alkylated by PNBM.[2],[3] The tagged substrates can be isolated by immunoprecipitation or immunoaffinity purification, or they can be detected by immunoblotting.
Reference:
[1]. Allen, J.J., Li, M., Brinkworth, C.S., et al. A semisynthetic epitope for kinase substrates. Nat. Methods 4(6), 511-516 (2007).
[2]. Kumar, V., Weng, Y.-C., Geldenhuys, W.J., et al. Generation and characterization of ATP analog-specific protein kinase Cδ. J. Biol. Chem. 290(4), 1936-1951 (2015).
[3]. Leissing, F., Nomoto, M., Bocola, M., et al. Substrate thiophosphorylation by Arabidopsis mitogen-activated protein kinases. BMC Plant Biol. 16(48), (2016).
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