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PA Janelia Fluor 549, SE (Synonyms: PA-JF549-NHS)

Catalog No.GC50361

Yellow photoactivatable dye (Reactive group: NHS ester). Applications: super resolution microscopy (PALM), single molecule tracking and live cell imaging

Products are for research use only. Not for human use. We do not sell to patients.

PA Janelia Fluor 549, SE Chemical Structure

Cas No.: 1811539-42-0

Size Price Stock Qty
300 ug
$271.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

Photoactivatable fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. Non-fluorescent until activated at 365 nm. Photochemical quantum yield of uncaging = 2.2%, with improved yield upon protein conjugation. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for single molecule tracking and super resolution microscopy in live cells, specifically live cell sptPALM and fixed cell PALM. Can be multiplexed with PA Janelia Fluor® 646, SE to perform two-color sptPALM in live cells with activation at 405 nm. Cell permeable. Excitation maximum = 551 - 553 nm; emission maximum = 570 - 573 nm. HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Grimm et al (2016) Bright photoactivatable fluorophores for single-molecule imaging. Nat.Methods 13 985 PMID:27776112 |Li et al (2016) Real-time imaging of Huntingtin aggregates diverting target search and gene transcription. eLife 5 e17056 PMID:27484239 |Hong et al (2009) Phosphorylation of the RNA polymerase II C-terminal domain by TFIIH kinase is not essential for transcription of Saccharomyces cerevisiae genome. Proc.Natl.Acad.Sci.USA. 106 14276 PMID:19666497

Chemical Properties

Cas No. 1811539-42-0 SDF
Synonyms PA-JF549-NHS
Canonical SMILES O=C(ON1C(CCC1=O)=O)C2=CC=C(C(C3=[N+]=[N-])=O)C(C43C5=CC=C(N6CCC6)C=C5OC7=CC(N8CCC8)=CC=C47)=C2
Formula C32H25N5O6 M.Wt 575.58
Solubility 5.76 mg/mL in DMSO Storage Store at -20°C; protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 1.7374 mL 8.6869 mL 17.3738 mL
5 mM 0.3475 mL 1.7374 mL 3.4748 mL
10 mM 0.1737 mL 0.8687 mL 1.7374 mL
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Reviews

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Average Rating: 5 ★★★★★ (Based on Reviews and 9 reference(s) in Google Scholar.)

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