Pam2CSK4 (trifluoroacetate salt) (Synonyms: Pam2Cys-Ser-(Lys)4) |
| Catalog No.GC49061 |
A bacterial lipopeptide and TLR2 agonist
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: N/A
Sample solution is provided at 25 µL, 10mM.
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Pam2CSK4 is a synthetic bacterial lipopeptide and an agonist of the toll-like receptor 2 (TLR2).1 It is selective for the TLR2/TLR6 heterodimer over TLR2/TLR1 (EC50s = 0.015 and 4.12 ng/ml, respectively, in reporter assays using human receptors). Pam2CSK4 increases the levels of inducible nitric oxide synthase (iNOS) and the production of nitric oxide (NO) in RAW 264.7 macrophages when used at a concentration of 100 ng/ml.2 It stimulates osteoclast formation, induces gingival inflammation, and decreases alveolar bone mass in a mouse model of periodontitis when administered at a dose of 5 µg/animal.3 Pam2CSK4 (10 µg/vaccine), when used as an adjuvant, reduces parasite burden and enhances the response to antigen, as measured by IgG levels, in a mouse model of lymphatic filariasis induced by B. malayi.4
1.Irvine, K.L., Hopkins, L.J., Gangloff, M., et al.The molecular basis for recognition of bacterial ligands at equine TLR2, TLR1 and TLR6Vet. Res.44(1)50(2013) 2.Kulsantiwong, P., Pudla, M., Srisaowakarn, C., et al.Pam2CSK4 and Pam3CSK4 induce iNOS expression via TBK1 and MyD88 molecules in mouse macrophage cell line RAW264.7Inflamm. Res.66(10)843-853(2017) 3.de Souza, J.A.C., MagalhÁes, F.A.C., de Oliveira, G.J.P.L., et al.Pam2CSK4 (TLR2 agonist) induces periodontal destruction in miceBraz. Oral. Res.34e012(2020) 4.Halliday, A., Turner, J.D., GuimarÃes, A., et al.The TLR2/6 ligand PAM2CSK4 is a Th2 polarizing adjuvant in Leishmania major and Brugia malayi murine vaccine modelsParasit. Vectors996(2016)
| Cas No. | N/A | SDF | |
| Synonyms | Pam2Cys-Ser-(Lys)4 | ||
| Canonical SMILES | CCCCCCCCCCCCCCCC(OC[C@@H](OC(CCCCCCCCCCCCCCC)=O)CSC[C@H](N)C(N[C@@H](CO)C(N[C@@H](CCCCN)C(N[C@@H](CCCCN)C(N[C@@H](CCCCN)C(N[C@H](C(O)=O)CCCCN)=O)=O)=O)=O)=O)=O.OC(C(F)(F)F)=O | ||
| Formula | C65H126N10O12S·XCF3COOH | M.Wt | 1271.8 |
| Solubility | Acetic Acid (0.25%): 1 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 0.7863 mL | 3.9314 mL | 7.8629 mL |
| 5 mM | 0.1573 mL | 0.7863 mL | 1.5726 mL |
| 10 mM | 0.0786 mL | 0.3931 mL | 0.7863 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 40 reference(s) in Google Scholar.)
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